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Determination of Clinical Characteristics of Mycobacterium kansasii-Derived Species by Reanalysis of Isolates Formerly Reported as M. kansasii

BACKGROUND: Seven genotypic subtypes of Mycobacterium kansasii were recently demonstrated to represent distinct species based on phylogenomic analysis. Mycobacterium kansasii sensu stricto (formerly known as subtype 1) is most frequently associated with human diseases; only a few studies have compar...

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Autores principales: Kim, Young-gon, Lee, Hong Yeul, Kwak, Nakwon, Park, Jae Hyeon, Kim, Taek Soo, Kim, Man Jin, Lee, Jee-Soo, Park, Sung-Sup, Yim, Jae-Joon, Seong, Moon-Woo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society for Laboratory Medicine 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8041593/
https://www.ncbi.nlm.nih.gov/pubmed/33824234
http://dx.doi.org/10.3343/alm.2021.41.5.463
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author Kim, Young-gon
Lee, Hong Yeul
Kwak, Nakwon
Park, Jae Hyeon
Kim, Taek Soo
Kim, Man Jin
Lee, Jee-Soo
Park, Sung-Sup
Yim, Jae-Joon
Seong, Moon-Woo
author_facet Kim, Young-gon
Lee, Hong Yeul
Kwak, Nakwon
Park, Jae Hyeon
Kim, Taek Soo
Kim, Man Jin
Lee, Jee-Soo
Park, Sung-Sup
Yim, Jae-Joon
Seong, Moon-Woo
author_sort Kim, Young-gon
collection PubMed
description BACKGROUND: Seven genotypic subtypes of Mycobacterium kansasii were recently demonstrated to represent distinct species based on phylogenomic analysis. Mycobacterium kansasii sensu stricto (formerly known as subtype 1) is most frequently associated with human diseases; only a few studies have compared the diverse clinical characteristics of M. kansasii subtypes, including their drug susceptibilities. We determined the actual incidence of infections caused by each subtype of M. kansasii and identified their clinical characteristics. METHODS: We subtyped isolates identified as M. kansasii over the last 10 years at a tertiary care hospital. Percent identity score of stored sequencing data was calculated using curated reference sequences of all M. kansasii subtypes. Clinical characteristics were compared between those classified as subtype 1 and other subtypes. Student’s t-test, Wilcoxon rank-sum test, and Fisher’s exact test were used for comparisons. RESULTS: Overall, 21.7% of the isolates were identified as species distinct from M. kansasii. The proportion of patients with subtype 1 M. kansasii infection who received treatment was significantly higher than that of patients with other subtype infections (55.3% vs. 7.7%, P=0.003). Only patients with subtype 1 infection received surgical treatment. Non-subtype 1 M. kansasii isolates showed a higher frequency of resistance to ciprofloxacin and trimethoprim/sulfamethoxazole. CONCLUSIONS: Non-subtype 1 M. kansasii isolates should be separately identified in routine clinical laboratory tests for appropriate treatment selection.
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spelling pubmed-80415932021-09-01 Determination of Clinical Characteristics of Mycobacterium kansasii-Derived Species by Reanalysis of Isolates Formerly Reported as M. kansasii Kim, Young-gon Lee, Hong Yeul Kwak, Nakwon Park, Jae Hyeon Kim, Taek Soo Kim, Man Jin Lee, Jee-Soo Park, Sung-Sup Yim, Jae-Joon Seong, Moon-Woo Ann Lab Med Original Article BACKGROUND: Seven genotypic subtypes of Mycobacterium kansasii were recently demonstrated to represent distinct species based on phylogenomic analysis. Mycobacterium kansasii sensu stricto (formerly known as subtype 1) is most frequently associated with human diseases; only a few studies have compared the diverse clinical characteristics of M. kansasii subtypes, including their drug susceptibilities. We determined the actual incidence of infections caused by each subtype of M. kansasii and identified their clinical characteristics. METHODS: We subtyped isolates identified as M. kansasii over the last 10 years at a tertiary care hospital. Percent identity score of stored sequencing data was calculated using curated reference sequences of all M. kansasii subtypes. Clinical characteristics were compared between those classified as subtype 1 and other subtypes. Student’s t-test, Wilcoxon rank-sum test, and Fisher’s exact test were used for comparisons. RESULTS: Overall, 21.7% of the isolates were identified as species distinct from M. kansasii. The proportion of patients with subtype 1 M. kansasii infection who received treatment was significantly higher than that of patients with other subtype infections (55.3% vs. 7.7%, P=0.003). Only patients with subtype 1 infection received surgical treatment. Non-subtype 1 M. kansasii isolates showed a higher frequency of resistance to ciprofloxacin and trimethoprim/sulfamethoxazole. CONCLUSIONS: Non-subtype 1 M. kansasii isolates should be separately identified in routine clinical laboratory tests for appropriate treatment selection. Korean Society for Laboratory Medicine 2021-09-01 2021-09-01 /pmc/articles/PMC8041593/ /pubmed/33824234 http://dx.doi.org/10.3343/alm.2021.41.5.463 Text en © Korean Society for Laboratory Medicine https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0 (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Kim, Young-gon
Lee, Hong Yeul
Kwak, Nakwon
Park, Jae Hyeon
Kim, Taek Soo
Kim, Man Jin
Lee, Jee-Soo
Park, Sung-Sup
Yim, Jae-Joon
Seong, Moon-Woo
Determination of Clinical Characteristics of Mycobacterium kansasii-Derived Species by Reanalysis of Isolates Formerly Reported as M. kansasii
title Determination of Clinical Characteristics of Mycobacterium kansasii-Derived Species by Reanalysis of Isolates Formerly Reported as M. kansasii
title_full Determination of Clinical Characteristics of Mycobacterium kansasii-Derived Species by Reanalysis of Isolates Formerly Reported as M. kansasii
title_fullStr Determination of Clinical Characteristics of Mycobacterium kansasii-Derived Species by Reanalysis of Isolates Formerly Reported as M. kansasii
title_full_unstemmed Determination of Clinical Characteristics of Mycobacterium kansasii-Derived Species by Reanalysis of Isolates Formerly Reported as M. kansasii
title_short Determination of Clinical Characteristics of Mycobacterium kansasii-Derived Species by Reanalysis of Isolates Formerly Reported as M. kansasii
title_sort determination of clinical characteristics of mycobacterium kansasii-derived species by reanalysis of isolates formerly reported as m. kansasii
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8041593/
https://www.ncbi.nlm.nih.gov/pubmed/33824234
http://dx.doi.org/10.3343/alm.2021.41.5.463
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