Astigmatic traction force microscopy (aTFM)

Quantifying small, rapidly progressing three-dimensional forces generated by cells remains a major challenge towards a more complete understanding of mechanobiology. Traction force microscopy is one of the most broadly applied force probing technologies but ascertaining three-dimensional information...

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Autores principales: Li, Di, Colin-York, Huw, Barbieri, Liliana, Javanmardi, Yousef, Guo, Yuting, Korobchevskaya, Kseniya, Moeendarbary, Emad, Li, Dong, Fritzsche, Marco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8042066/
https://www.ncbi.nlm.nih.gov/pubmed/33846322
http://dx.doi.org/10.1038/s41467-021-22376-w
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author Li, Di
Colin-York, Huw
Barbieri, Liliana
Javanmardi, Yousef
Guo, Yuting
Korobchevskaya, Kseniya
Moeendarbary, Emad
Li, Dong
Fritzsche, Marco
author_facet Li, Di
Colin-York, Huw
Barbieri, Liliana
Javanmardi, Yousef
Guo, Yuting
Korobchevskaya, Kseniya
Moeendarbary, Emad
Li, Dong
Fritzsche, Marco
author_sort Li, Di
collection PubMed
description Quantifying small, rapidly progressing three-dimensional forces generated by cells remains a major challenge towards a more complete understanding of mechanobiology. Traction force microscopy is one of the most broadly applied force probing technologies but ascertaining three-dimensional information typically necessitates slow, multi-frame z-stack acquisition with limited sensitivity. Here, by performing traction force microscopy using fast single-frame astigmatic imaging coupled with total internal reflection fluorescence microscopy we improve the temporal resolution of three-dimensional mechanical force quantification up to 10-fold compared to its related super-resolution modalities. 2.5D astigmatic traction force microscopy (aTFM) thus enables live-cell force measurements approaching physiological sensitivity.
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spelling pubmed-80420662021-04-30 Astigmatic traction force microscopy (aTFM) Li, Di Colin-York, Huw Barbieri, Liliana Javanmardi, Yousef Guo, Yuting Korobchevskaya, Kseniya Moeendarbary, Emad Li, Dong Fritzsche, Marco Nat Commun Article Quantifying small, rapidly progressing three-dimensional forces generated by cells remains a major challenge towards a more complete understanding of mechanobiology. Traction force microscopy is one of the most broadly applied force probing technologies but ascertaining three-dimensional information typically necessitates slow, multi-frame z-stack acquisition with limited sensitivity. Here, by performing traction force microscopy using fast single-frame astigmatic imaging coupled with total internal reflection fluorescence microscopy we improve the temporal resolution of three-dimensional mechanical force quantification up to 10-fold compared to its related super-resolution modalities. 2.5D astigmatic traction force microscopy (aTFM) thus enables live-cell force measurements approaching physiological sensitivity. Nature Publishing Group UK 2021-04-12 /pmc/articles/PMC8042066/ /pubmed/33846322 http://dx.doi.org/10.1038/s41467-021-22376-w Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Li, Di
Colin-York, Huw
Barbieri, Liliana
Javanmardi, Yousef
Guo, Yuting
Korobchevskaya, Kseniya
Moeendarbary, Emad
Li, Dong
Fritzsche, Marco
Astigmatic traction force microscopy (aTFM)
title Astigmatic traction force microscopy (aTFM)
title_full Astigmatic traction force microscopy (aTFM)
title_fullStr Astigmatic traction force microscopy (aTFM)
title_full_unstemmed Astigmatic traction force microscopy (aTFM)
title_short Astigmatic traction force microscopy (aTFM)
title_sort astigmatic traction force microscopy (atfm)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8042066/
https://www.ncbi.nlm.nih.gov/pubmed/33846322
http://dx.doi.org/10.1038/s41467-021-22376-w
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