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Role of Cigarette Smoke on Angiotensin-Converting Enzyme-2 Protein Membrane Expression in Bronchial Epithelial Cells Using an Air-Liquid Interface Model

Prevalence studies of current smoking, among hospitalized COVID-19 patients, demonstrated an unexpectedly low prevalence among patients with COVID-19. The aim of the present study was to evaluate the effect of smoke from cigarettes on ACE-2 in bronchial epithelial cells. Normal bronchial epithelial...

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Autores principales: Caruso, Massimo, Distefano, Alfio, Emma, Rosalia, Di Rosa, Michelino, Carota, Giuseppe, Rust, Sonja, Polosa, Riccardo, Zuccarello, Pietro, Ferrante, Margherita, Raciti, Giuseppina, Li Volti, Giovanni
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8042260/
https://www.ncbi.nlm.nih.gov/pubmed/33859566
http://dx.doi.org/10.3389/fphar.2021.652102
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author Caruso, Massimo
Distefano, Alfio
Emma, Rosalia
Di Rosa, Michelino
Carota, Giuseppe
Rust, Sonja
Polosa, Riccardo
Zuccarello, Pietro
Ferrante, Margherita
Raciti, Giuseppina
Li Volti, Giovanni
author_facet Caruso, Massimo
Distefano, Alfio
Emma, Rosalia
Di Rosa, Michelino
Carota, Giuseppe
Rust, Sonja
Polosa, Riccardo
Zuccarello, Pietro
Ferrante, Margherita
Raciti, Giuseppina
Li Volti, Giovanni
author_sort Caruso, Massimo
collection PubMed
description Prevalence studies of current smoking, among hospitalized COVID-19 patients, demonstrated an unexpectedly low prevalence among patients with COVID-19. The aim of the present study was to evaluate the effect of smoke from cigarettes on ACE-2 in bronchial epithelial cells. Normal bronchial epithelial cells (H292) were exposed to smoke by an air-liquid-interface (ALI) system and ACE-2 membrane protein expression was evaluated after 24 h from exposure. Our transcriptomics data analysis showed a significant selective reduction of membrane ACE-2 expression (about 25%) following smoking exposure. Interestingly, we observed a positive direct correlation between ACE-2 reduction and nicotine delivery. Furthermore, by stratifying GSE52237 as a function of ACE-2 gene expression levels, we highlighted 1,012 genes related to ACE-2 in smokers and 855 in non-smokers. Furthermore, we showed that 161 genes involved in the endocytosis process were highlighted using the online pathway tool KEGG. Finally, 11 genes were in common between the ACE-2 pathway in smokers and the genes regulated during endocytosis, while 12 genes with non-smokers. Interestingly, six in non-smokers and four genes in smokers were closely involved during the viral internalization process. Our data may offer a pharmaceutical role of nicotine as potential treatment option in COVID-19.
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spelling pubmed-80422602021-04-14 Role of Cigarette Smoke on Angiotensin-Converting Enzyme-2 Protein Membrane Expression in Bronchial Epithelial Cells Using an Air-Liquid Interface Model Caruso, Massimo Distefano, Alfio Emma, Rosalia Di Rosa, Michelino Carota, Giuseppe Rust, Sonja Polosa, Riccardo Zuccarello, Pietro Ferrante, Margherita Raciti, Giuseppina Li Volti, Giovanni Front Pharmacol Pharmacology Prevalence studies of current smoking, among hospitalized COVID-19 patients, demonstrated an unexpectedly low prevalence among patients with COVID-19. The aim of the present study was to evaluate the effect of smoke from cigarettes on ACE-2 in bronchial epithelial cells. Normal bronchial epithelial cells (H292) were exposed to smoke by an air-liquid-interface (ALI) system and ACE-2 membrane protein expression was evaluated after 24 h from exposure. Our transcriptomics data analysis showed a significant selective reduction of membrane ACE-2 expression (about 25%) following smoking exposure. Interestingly, we observed a positive direct correlation between ACE-2 reduction and nicotine delivery. Furthermore, by stratifying GSE52237 as a function of ACE-2 gene expression levels, we highlighted 1,012 genes related to ACE-2 in smokers and 855 in non-smokers. Furthermore, we showed that 161 genes involved in the endocytosis process were highlighted using the online pathway tool KEGG. Finally, 11 genes were in common between the ACE-2 pathway in smokers and the genes regulated during endocytosis, while 12 genes with non-smokers. Interestingly, six in non-smokers and four genes in smokers were closely involved during the viral internalization process. Our data may offer a pharmaceutical role of nicotine as potential treatment option in COVID-19. Frontiers Media S.A. 2021-03-30 /pmc/articles/PMC8042260/ /pubmed/33859566 http://dx.doi.org/10.3389/fphar.2021.652102 Text en Copyright © 2021 Caruso, Distefano, Emma, Di Rosa, Carota, Rust, Polosa, Zuccarello, Ferrante, Raciti and Li Volti. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Caruso, Massimo
Distefano, Alfio
Emma, Rosalia
Di Rosa, Michelino
Carota, Giuseppe
Rust, Sonja
Polosa, Riccardo
Zuccarello, Pietro
Ferrante, Margherita
Raciti, Giuseppina
Li Volti, Giovanni
Role of Cigarette Smoke on Angiotensin-Converting Enzyme-2 Protein Membrane Expression in Bronchial Epithelial Cells Using an Air-Liquid Interface Model
title Role of Cigarette Smoke on Angiotensin-Converting Enzyme-2 Protein Membrane Expression in Bronchial Epithelial Cells Using an Air-Liquid Interface Model
title_full Role of Cigarette Smoke on Angiotensin-Converting Enzyme-2 Protein Membrane Expression in Bronchial Epithelial Cells Using an Air-Liquid Interface Model
title_fullStr Role of Cigarette Smoke on Angiotensin-Converting Enzyme-2 Protein Membrane Expression in Bronchial Epithelial Cells Using an Air-Liquid Interface Model
title_full_unstemmed Role of Cigarette Smoke on Angiotensin-Converting Enzyme-2 Protein Membrane Expression in Bronchial Epithelial Cells Using an Air-Liquid Interface Model
title_short Role of Cigarette Smoke on Angiotensin-Converting Enzyme-2 Protein Membrane Expression in Bronchial Epithelial Cells Using an Air-Liquid Interface Model
title_sort role of cigarette smoke on angiotensin-converting enzyme-2 protein membrane expression in bronchial epithelial cells using an air-liquid interface model
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8042260/
https://www.ncbi.nlm.nih.gov/pubmed/33859566
http://dx.doi.org/10.3389/fphar.2021.652102
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