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Supplementing Blood Diet With Plant Nectar Enhances Egg Fertility in Stomoxys calcitrans
Stomoxys calcitrans (stable fly) is a cosmopolitan biting fly of both medical and veterinary importance. Unlike blood-feeding-related behavior of stable fly, its plant feeding, the fitness value, and the S. calcitrans–plant interaction are less understood. Here we show based on two chloroplast DNA g...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8042263/ https://www.ncbi.nlm.nih.gov/pubmed/33859570 http://dx.doi.org/10.3389/fphys.2021.646367 |
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author | Tawich, Simon K. Bargul, Joel L. Masiga, Daniel Getahun, Merid N. |
author_facet | Tawich, Simon K. Bargul, Joel L. Masiga, Daniel Getahun, Merid N. |
author_sort | Tawich, Simon K. |
collection | PubMed |
description | Stomoxys calcitrans (stable fly) is a cosmopolitan biting fly of both medical and veterinary importance. Unlike blood-feeding-related behavior of stable fly, its plant feeding, the fitness value, and the S. calcitrans–plant interaction are less understood. Here we show based on two chloroplast DNA genes, ribulose bisphosphate carboxylase large chain (rbcL) and the intergenic spacer gene trnH-psbA, that field-collected male and female stable flies fed on various plant species. We investigated the fitness cost of plant feeding using Parthenium hysterophorus, one of the plant species identified to have been fed on by the field-collected flies. Supplementation of blood feeding with a flowering P. hysterophorus plant as nectar source enhanced egg hatchability significantly as compared to blood alone, showing the fitness value of nectar supplementation. However, nectar supplementation did not affect the number of eggs laid or longevity of S. calcitrans as compared to flies that fed on blood alone. S. calcitrans maintained on sugar alone failed to lay eggs. The various plants stable flies fed on demonstrated chemodiversity with their own signature scent. The behavioral response of S. calcitrans to these signature compounds varied from strong attraction (γ-terpinene) to neutral (linalool oxide and myrcene) to repellency (butanoic acid). Our study demonstrated that stable flies feed on nectar, and plant nectar supplementation of blood feeding enhanced larval emergence. Thus, our result has implication in stable fly reproduction, survival, disease transmission, boosting laboratory colony, and the possibility of using plant-derived odors for mass trapping of stable fly, for instance, using γ-terpinene. |
format | Online Article Text |
id | pubmed-8042263 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-80422632021-04-14 Supplementing Blood Diet With Plant Nectar Enhances Egg Fertility in Stomoxys calcitrans Tawich, Simon K. Bargul, Joel L. Masiga, Daniel Getahun, Merid N. Front Physiol Physiology Stomoxys calcitrans (stable fly) is a cosmopolitan biting fly of both medical and veterinary importance. Unlike blood-feeding-related behavior of stable fly, its plant feeding, the fitness value, and the S. calcitrans–plant interaction are less understood. Here we show based on two chloroplast DNA genes, ribulose bisphosphate carboxylase large chain (rbcL) and the intergenic spacer gene trnH-psbA, that field-collected male and female stable flies fed on various plant species. We investigated the fitness cost of plant feeding using Parthenium hysterophorus, one of the plant species identified to have been fed on by the field-collected flies. Supplementation of blood feeding with a flowering P. hysterophorus plant as nectar source enhanced egg hatchability significantly as compared to blood alone, showing the fitness value of nectar supplementation. However, nectar supplementation did not affect the number of eggs laid or longevity of S. calcitrans as compared to flies that fed on blood alone. S. calcitrans maintained on sugar alone failed to lay eggs. The various plants stable flies fed on demonstrated chemodiversity with their own signature scent. The behavioral response of S. calcitrans to these signature compounds varied from strong attraction (γ-terpinene) to neutral (linalool oxide and myrcene) to repellency (butanoic acid). Our study demonstrated that stable flies feed on nectar, and plant nectar supplementation of blood feeding enhanced larval emergence. Thus, our result has implication in stable fly reproduction, survival, disease transmission, boosting laboratory colony, and the possibility of using plant-derived odors for mass trapping of stable fly, for instance, using γ-terpinene. Frontiers Media S.A. 2021-03-30 /pmc/articles/PMC8042263/ /pubmed/33859570 http://dx.doi.org/10.3389/fphys.2021.646367 Text en Copyright © 2021 Tawich, Bargul, Masiga and Getahun. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Physiology Tawich, Simon K. Bargul, Joel L. Masiga, Daniel Getahun, Merid N. Supplementing Blood Diet With Plant Nectar Enhances Egg Fertility in Stomoxys calcitrans |
title | Supplementing Blood Diet With Plant Nectar Enhances Egg Fertility in Stomoxys calcitrans |
title_full | Supplementing Blood Diet With Plant Nectar Enhances Egg Fertility in Stomoxys calcitrans |
title_fullStr | Supplementing Blood Diet With Plant Nectar Enhances Egg Fertility in Stomoxys calcitrans |
title_full_unstemmed | Supplementing Blood Diet With Plant Nectar Enhances Egg Fertility in Stomoxys calcitrans |
title_short | Supplementing Blood Diet With Plant Nectar Enhances Egg Fertility in Stomoxys calcitrans |
title_sort | supplementing blood diet with plant nectar enhances egg fertility in stomoxys calcitrans |
topic | Physiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8042263/ https://www.ncbi.nlm.nih.gov/pubmed/33859570 http://dx.doi.org/10.3389/fphys.2021.646367 |
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