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Maternal HLA Ib Polymorphisms in Pregnancy Allo-Immunization

During pregnancy the formation of alloreactive anti-human leukocyte antigen (HLA) antibodies are a major cause of acute rejection in organ transplantation and of adverse effects in blood transfusion. The purpose of the study was to identify maternal HLA class Ib genetic factors associated with anti-...

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Autores principales: Persson, Gry, Picard, Christophe, Marin, Gregory, Isgaard, Cecilie, Stæhr, Christina Seefeldt, Molinari, Nicolas, Chiaroni, Jacques, Lebech, Morten, Hviid, Thomas Vauvert F., Di Cristofaro, Julie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8042285/
https://www.ncbi.nlm.nih.gov/pubmed/33859649
http://dx.doi.org/10.3389/fimmu.2021.657217
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author Persson, Gry
Picard, Christophe
Marin, Gregory
Isgaard, Cecilie
Stæhr, Christina Seefeldt
Molinari, Nicolas
Chiaroni, Jacques
Lebech, Morten
Hviid, Thomas Vauvert F.
Di Cristofaro, Julie
author_facet Persson, Gry
Picard, Christophe
Marin, Gregory
Isgaard, Cecilie
Stæhr, Christina Seefeldt
Molinari, Nicolas
Chiaroni, Jacques
Lebech, Morten
Hviid, Thomas Vauvert F.
Di Cristofaro, Julie
author_sort Persson, Gry
collection PubMed
description During pregnancy the formation of alloreactive anti-human leukocyte antigen (HLA) antibodies are a major cause of acute rejection in organ transplantation and of adverse effects in blood transfusion. The purpose of the study was to identify maternal HLA class Ib genetic factors associated with anti-HLA allo-immunization in pregnancy and the degree of tolerance estimated by IgG4 expression. In total, 86 primiparous women with singleton pregnancies were included in the study. Maternal blood samples and umbilical cord samples were collected at delivery. Clinical data were obtained. Maternal blood serum was screened for HLA class I and II antibodies, identification of Donor Specific Antibody (DSA), activation of complement measured by C1q and IgG4 concentrations. Mothers were genotyped for HLA class Ib (HLA-E, -F and -G). Anti-HLA class I and II antibodies were identified in 24% of the women. The maternal HLA-E*01:06 allele was significantly associated with a higher fraction of anti-HLA I immunization (20.0% vs. 4.8%, p = 0.048). The maternal HLA-G 3’-untranslated region UTR4-HLA-G*01:01:01:05 haplotype and the HLA-F*01:03:01 allele were significantly associated with a low anti-HLA I C1q activation (16.7% vs. 57.1%, p = 0.028; 16.7% vs. 50.0%, p = 0.046; respectively). Both HLA‑G and HLA-F*01:03:01 showed significantly higher levels of IgG4 compared with the other haplotypes. The results support an association of certain HLA class Ib alleles with allo-immunization during pregnancy. Further studies are needed to elucidate the roles of HLA-E*01:06, HLA-F*01:03 and HLA‑G UTR4 in reducing the risk for allo-immunization.
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spelling pubmed-80422852021-04-14 Maternal HLA Ib Polymorphisms in Pregnancy Allo-Immunization Persson, Gry Picard, Christophe Marin, Gregory Isgaard, Cecilie Stæhr, Christina Seefeldt Molinari, Nicolas Chiaroni, Jacques Lebech, Morten Hviid, Thomas Vauvert F. Di Cristofaro, Julie Front Immunol Immunology During pregnancy the formation of alloreactive anti-human leukocyte antigen (HLA) antibodies are a major cause of acute rejection in organ transplantation and of adverse effects in blood transfusion. The purpose of the study was to identify maternal HLA class Ib genetic factors associated with anti-HLA allo-immunization in pregnancy and the degree of tolerance estimated by IgG4 expression. In total, 86 primiparous women with singleton pregnancies were included in the study. Maternal blood samples and umbilical cord samples were collected at delivery. Clinical data were obtained. Maternal blood serum was screened for HLA class I and II antibodies, identification of Donor Specific Antibody (DSA), activation of complement measured by C1q and IgG4 concentrations. Mothers were genotyped for HLA class Ib (HLA-E, -F and -G). Anti-HLA class I and II antibodies were identified in 24% of the women. The maternal HLA-E*01:06 allele was significantly associated with a higher fraction of anti-HLA I immunization (20.0% vs. 4.8%, p = 0.048). The maternal HLA-G 3’-untranslated region UTR4-HLA-G*01:01:01:05 haplotype and the HLA-F*01:03:01 allele were significantly associated with a low anti-HLA I C1q activation (16.7% vs. 57.1%, p = 0.028; 16.7% vs. 50.0%, p = 0.046; respectively). Both HLA‑G and HLA-F*01:03:01 showed significantly higher levels of IgG4 compared with the other haplotypes. The results support an association of certain HLA class Ib alleles with allo-immunization during pregnancy. Further studies are needed to elucidate the roles of HLA-E*01:06, HLA-F*01:03 and HLA‑G UTR4 in reducing the risk for allo-immunization. Frontiers Media S.A. 2021-03-30 /pmc/articles/PMC8042285/ /pubmed/33859649 http://dx.doi.org/10.3389/fimmu.2021.657217 Text en Copyright © 2021 Persson, Picard, Marin, Isgaard, Stæhr, Molinari, Chiaroni, Lebech, Hviid and Di Cristofaro https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Persson, Gry
Picard, Christophe
Marin, Gregory
Isgaard, Cecilie
Stæhr, Christina Seefeldt
Molinari, Nicolas
Chiaroni, Jacques
Lebech, Morten
Hviid, Thomas Vauvert F.
Di Cristofaro, Julie
Maternal HLA Ib Polymorphisms in Pregnancy Allo-Immunization
title Maternal HLA Ib Polymorphisms in Pregnancy Allo-Immunization
title_full Maternal HLA Ib Polymorphisms in Pregnancy Allo-Immunization
title_fullStr Maternal HLA Ib Polymorphisms in Pregnancy Allo-Immunization
title_full_unstemmed Maternal HLA Ib Polymorphisms in Pregnancy Allo-Immunization
title_short Maternal HLA Ib Polymorphisms in Pregnancy Allo-Immunization
title_sort maternal hla ib polymorphisms in pregnancy allo-immunization
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8042285/
https://www.ncbi.nlm.nih.gov/pubmed/33859649
http://dx.doi.org/10.3389/fimmu.2021.657217
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