Cargando…

Integrated Genomic and Greenhouse Assessment of a Novel Plant Growth-Promoting Rhizobacterium for Tomato Plant

Plant growth promoting rhizobacteria (PGPR) can display several plant-beneficial properties, including support to plant nutrition, regulation of plant growth, and biocontrol of pests. Mechanisms behind these effects are directly related to the presence and expression of specific genes, and different...

Descripción completa

Detalles Bibliográficos
Autores principales: Guerrieri, Maria Chiara, Fiorini, Andrea, Fanfoni, Elisabetta, Tabaglio, Vincenzo, Cocconcelli, Pier Sandro, Trevisan, Marco, Puglisi, Edoardo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8042378/
https://www.ncbi.nlm.nih.gov/pubmed/33859664
http://dx.doi.org/10.3389/fpls.2021.660620
_version_ 1783678114838085632
author Guerrieri, Maria Chiara
Fiorini, Andrea
Fanfoni, Elisabetta
Tabaglio, Vincenzo
Cocconcelli, Pier Sandro
Trevisan, Marco
Puglisi, Edoardo
author_facet Guerrieri, Maria Chiara
Fiorini, Andrea
Fanfoni, Elisabetta
Tabaglio, Vincenzo
Cocconcelli, Pier Sandro
Trevisan, Marco
Puglisi, Edoardo
author_sort Guerrieri, Maria Chiara
collection PubMed
description Plant growth promoting rhizobacteria (PGPR) can display several plant-beneficial properties, including support to plant nutrition, regulation of plant growth, and biocontrol of pests. Mechanisms behind these effects are directly related to the presence and expression of specific genes, and different PGPR strains can be differentiated by the presence of different genes. In this study we reported a comprehensive evaluation of a novel PGPR Klebsiella variicola UC4115 from the field to the lab, and from the lab to the plant. The isolate from tomato field was screened in-vitro for different activities related to plant nutrition and growth regulation as well as for antifungal traits. We performed a functional annotation of genes contributing to plant-beneficial functions previously tested in-vitro. Furthermore, the in-vitro characterization, the whole genome sequencing and annotation of K. variicola UC4115, were compared with the well-known PGPR Azospirillum brasilense strain Sp7. This novel comparative analysis revealed different accumulation of plant-beneficial functions contributing genes, and the presence of different genes that accomplished the same functions. Greenhouse assays on tomato seedlings from BBCH 11–12 to BBCH > 14 were performed under either organic or conventional management. In each of them, three PGPR inoculations (control, K. variicola UC4115, A. brasilense Sp7) were applied at either seed-, root-, and seed plus root level. Results confirmed the PGP potential of K. variicola UC4115; in particular, its high value potential as indole-3-acetic acid producer was observed in increasing of root length density and diameter class length parameters. While, in general, A. brasilense Sp7 had a greater effect on biomass, probably due to its high ability as nitrogen-fixing bacteria. For K. variicola UC4115, the most consistent data were noticed under organic management, with application at seed level. While, A. brasilense Sp7 showed the greatest performance under conventional management. Our data highlight the necessity to tailor the selected PGPR, with the mode of inoculation and the crop-soil combination.
format Online
Article
Text
id pubmed-8042378
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-80423782021-04-14 Integrated Genomic and Greenhouse Assessment of a Novel Plant Growth-Promoting Rhizobacterium for Tomato Plant Guerrieri, Maria Chiara Fiorini, Andrea Fanfoni, Elisabetta Tabaglio, Vincenzo Cocconcelli, Pier Sandro Trevisan, Marco Puglisi, Edoardo Front Plant Sci Plant Science Plant growth promoting rhizobacteria (PGPR) can display several plant-beneficial properties, including support to plant nutrition, regulation of plant growth, and biocontrol of pests. Mechanisms behind these effects are directly related to the presence and expression of specific genes, and different PGPR strains can be differentiated by the presence of different genes. In this study we reported a comprehensive evaluation of a novel PGPR Klebsiella variicola UC4115 from the field to the lab, and from the lab to the plant. The isolate from tomato field was screened in-vitro for different activities related to plant nutrition and growth regulation as well as for antifungal traits. We performed a functional annotation of genes contributing to plant-beneficial functions previously tested in-vitro. Furthermore, the in-vitro characterization, the whole genome sequencing and annotation of K. variicola UC4115, were compared with the well-known PGPR Azospirillum brasilense strain Sp7. This novel comparative analysis revealed different accumulation of plant-beneficial functions contributing genes, and the presence of different genes that accomplished the same functions. Greenhouse assays on tomato seedlings from BBCH 11–12 to BBCH > 14 were performed under either organic or conventional management. In each of them, three PGPR inoculations (control, K. variicola UC4115, A. brasilense Sp7) were applied at either seed-, root-, and seed plus root level. Results confirmed the PGP potential of K. variicola UC4115; in particular, its high value potential as indole-3-acetic acid producer was observed in increasing of root length density and diameter class length parameters. While, in general, A. brasilense Sp7 had a greater effect on biomass, probably due to its high ability as nitrogen-fixing bacteria. For K. variicola UC4115, the most consistent data were noticed under organic management, with application at seed level. While, A. brasilense Sp7 showed the greatest performance under conventional management. Our data highlight the necessity to tailor the selected PGPR, with the mode of inoculation and the crop-soil combination. Frontiers Media S.A. 2021-03-30 /pmc/articles/PMC8042378/ /pubmed/33859664 http://dx.doi.org/10.3389/fpls.2021.660620 Text en Copyright © 2021 Guerrieri, Fiorini, Fanfoni, Tabaglio, Cocconcelli, Trevisan and Puglisi. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Guerrieri, Maria Chiara
Fiorini, Andrea
Fanfoni, Elisabetta
Tabaglio, Vincenzo
Cocconcelli, Pier Sandro
Trevisan, Marco
Puglisi, Edoardo
Integrated Genomic and Greenhouse Assessment of a Novel Plant Growth-Promoting Rhizobacterium for Tomato Plant
title Integrated Genomic and Greenhouse Assessment of a Novel Plant Growth-Promoting Rhizobacterium for Tomato Plant
title_full Integrated Genomic and Greenhouse Assessment of a Novel Plant Growth-Promoting Rhizobacterium for Tomato Plant
title_fullStr Integrated Genomic and Greenhouse Assessment of a Novel Plant Growth-Promoting Rhizobacterium for Tomato Plant
title_full_unstemmed Integrated Genomic and Greenhouse Assessment of a Novel Plant Growth-Promoting Rhizobacterium for Tomato Plant
title_short Integrated Genomic and Greenhouse Assessment of a Novel Plant Growth-Promoting Rhizobacterium for Tomato Plant
title_sort integrated genomic and greenhouse assessment of a novel plant growth-promoting rhizobacterium for tomato plant
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8042378/
https://www.ncbi.nlm.nih.gov/pubmed/33859664
http://dx.doi.org/10.3389/fpls.2021.660620
work_keys_str_mv AT guerrierimariachiara integratedgenomicandgreenhouseassessmentofanovelplantgrowthpromotingrhizobacteriumfortomatoplant
AT fioriniandrea integratedgenomicandgreenhouseassessmentofanovelplantgrowthpromotingrhizobacteriumfortomatoplant
AT fanfonielisabetta integratedgenomicandgreenhouseassessmentofanovelplantgrowthpromotingrhizobacteriumfortomatoplant
AT tabagliovincenzo integratedgenomicandgreenhouseassessmentofanovelplantgrowthpromotingrhizobacteriumfortomatoplant
AT cocconcellipiersandro integratedgenomicandgreenhouseassessmentofanovelplantgrowthpromotingrhizobacteriumfortomatoplant
AT trevisanmarco integratedgenomicandgreenhouseassessmentofanovelplantgrowthpromotingrhizobacteriumfortomatoplant
AT puglisiedoardo integratedgenomicandgreenhouseassessmentofanovelplantgrowthpromotingrhizobacteriumfortomatoplant