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Odorant Receptors for Detecting Flowering Plant Cues Are Functionally Conserved across Moths and Butterflies
Odorant receptors (ORs) are essential for plant–insect interactions. However, despite the global impacts of Lepidoptera (moths and butterflies) as major herbivores and pollinators, little functional data are available about Lepidoptera ORs involved in plant-volatile detection. Here, we initially cha...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8042770/ https://www.ncbi.nlm.nih.gov/pubmed/33231630 http://dx.doi.org/10.1093/molbev/msaa300 |
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author | Guo, Mengbo Du, Lixiao Chen, Qiuyan Feng, Yilu Zhang, Jin Zhang, Xiaxuan Tian, Ke Cao, Song Huang, Tianyu Jacquin-Joly, Emmanuelle Wang, Guirong Liu, Yang |
author_facet | Guo, Mengbo Du, Lixiao Chen, Qiuyan Feng, Yilu Zhang, Jin Zhang, Xiaxuan Tian, Ke Cao, Song Huang, Tianyu Jacquin-Joly, Emmanuelle Wang, Guirong Liu, Yang |
author_sort | Guo, Mengbo |
collection | PubMed |
description | Odorant receptors (ORs) are essential for plant–insect interactions. However, despite the global impacts of Lepidoptera (moths and butterflies) as major herbivores and pollinators, little functional data are available about Lepidoptera ORs involved in plant-volatile detection. Here, we initially characterized the plant-volatile-sensing function(s) of 44 ORs from the cotton bollworm Helicoverpa armigera, and subsequently conducted a large-scale comparative analysis that establishes how most orthologous ORs have functionally diverged among closely related species whereas some rare ORs are functionally conserved. Specifically, our systematic analysis of H. armigera ORs cataloged the wide functional scope of the H. armigera OR repertoire, and also showed that HarmOR42 and its Spodoptera littoralis ortholog are functionally conserved. Pursuing this, we characterized the HarmOR42-orthologous ORs from 11 species across the Glossata suborder and confirmed the HarmOR42 orthologs form a unique OR lineage that has undergone strong purifying selection in Glossata species and whose members are tuned with strong specificity to phenylacetaldehyde, a floral scent component common to most angiosperms. In vivo studies via HarmOR42 knockout support that HarmOR42-related ORs are essential for host-detection by sensing phenylacetaldehyde. Our work also supports that these ORs coevolved with the tube-like proboscis, and has maintained functional stability throughout the long-term coexistence of Lepidoptera with angiosperms. Thus, beyond providing a rich empirical resource for delineating the precise functions of H. armigera ORs, our results enable a comparative analysis of insect ORs that have apparently facilitated and currently sustain the intimate adaptations and ecological interactions among nectar feeding insects and flowering plants. |
format | Online Article Text |
id | pubmed-8042770 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-80427702021-04-16 Odorant Receptors for Detecting Flowering Plant Cues Are Functionally Conserved across Moths and Butterflies Guo, Mengbo Du, Lixiao Chen, Qiuyan Feng, Yilu Zhang, Jin Zhang, Xiaxuan Tian, Ke Cao, Song Huang, Tianyu Jacquin-Joly, Emmanuelle Wang, Guirong Liu, Yang Mol Biol Evol Discoveries Odorant receptors (ORs) are essential for plant–insect interactions. However, despite the global impacts of Lepidoptera (moths and butterflies) as major herbivores and pollinators, little functional data are available about Lepidoptera ORs involved in plant-volatile detection. Here, we initially characterized the plant-volatile-sensing function(s) of 44 ORs from the cotton bollworm Helicoverpa armigera, and subsequently conducted a large-scale comparative analysis that establishes how most orthologous ORs have functionally diverged among closely related species whereas some rare ORs are functionally conserved. Specifically, our systematic analysis of H. armigera ORs cataloged the wide functional scope of the H. armigera OR repertoire, and also showed that HarmOR42 and its Spodoptera littoralis ortholog are functionally conserved. Pursuing this, we characterized the HarmOR42-orthologous ORs from 11 species across the Glossata suborder and confirmed the HarmOR42 orthologs form a unique OR lineage that has undergone strong purifying selection in Glossata species and whose members are tuned with strong specificity to phenylacetaldehyde, a floral scent component common to most angiosperms. In vivo studies via HarmOR42 knockout support that HarmOR42-related ORs are essential for host-detection by sensing phenylacetaldehyde. Our work also supports that these ORs coevolved with the tube-like proboscis, and has maintained functional stability throughout the long-term coexistence of Lepidoptera with angiosperms. Thus, beyond providing a rich empirical resource for delineating the precise functions of H. armigera ORs, our results enable a comparative analysis of insect ORs that have apparently facilitated and currently sustain the intimate adaptations and ecological interactions among nectar feeding insects and flowering plants. Oxford University Press 2020-11-24 /pmc/articles/PMC8042770/ /pubmed/33231630 http://dx.doi.org/10.1093/molbev/msaa300 Text en © The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Discoveries Guo, Mengbo Du, Lixiao Chen, Qiuyan Feng, Yilu Zhang, Jin Zhang, Xiaxuan Tian, Ke Cao, Song Huang, Tianyu Jacquin-Joly, Emmanuelle Wang, Guirong Liu, Yang Odorant Receptors for Detecting Flowering Plant Cues Are Functionally Conserved across Moths and Butterflies |
title | Odorant Receptors for Detecting Flowering Plant Cues Are Functionally Conserved across Moths and Butterflies |
title_full | Odorant Receptors for Detecting Flowering Plant Cues Are Functionally Conserved across Moths and Butterflies |
title_fullStr | Odorant Receptors for Detecting Flowering Plant Cues Are Functionally Conserved across Moths and Butterflies |
title_full_unstemmed | Odorant Receptors for Detecting Flowering Plant Cues Are Functionally Conserved across Moths and Butterflies |
title_short | Odorant Receptors for Detecting Flowering Plant Cues Are Functionally Conserved across Moths and Butterflies |
title_sort | odorant receptors for detecting flowering plant cues are functionally conserved across moths and butterflies |
topic | Discoveries |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8042770/ https://www.ncbi.nlm.nih.gov/pubmed/33231630 http://dx.doi.org/10.1093/molbev/msaa300 |
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