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MiR-33a plays an crucial role in the proliferation of bovine preadipocytes
Preadipocyte proliferation is a critical and precisely orchestrated procedure in adipogenesis, which is highly regulated by microRNAs (miRNAs). A previous study identified that the expression of miR-33a is different in intramuscular fat (IMF) tissues from steers and bulls. In the present study, miR-...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8043176/ https://www.ncbi.nlm.nih.gov/pubmed/33840361 http://dx.doi.org/10.1080/21623945.2021.1908655 |
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author | Zhang, Wenzhen Wang, Li Raza, Sayed Haidar Abbas Wang, Xiaoyu Wang, Guohu Liang, Chengcheng Cheng, Gong Li, Bingzhi Zan, Linsen |
author_facet | Zhang, Wenzhen Wang, Li Raza, Sayed Haidar Abbas Wang, Xiaoyu Wang, Guohu Liang, Chengcheng Cheng, Gong Li, Bingzhi Zan, Linsen |
author_sort | Zhang, Wenzhen |
collection | PubMed |
description | Preadipocyte proliferation is a critical and precisely orchestrated procedure in adipogenesis, which is highly regulated by microRNAs (miRNAs). A previous study identified that the expression of miR-33a is different in intramuscular fat (IMF) tissues from steers and bulls. In the present study, miR-33a was overexpressed in bovine preadipocytes, and a total of 781 differentialy expressed genes were found, including 348 upregulated and 433 downregulated genes. Gene Ontology and Kyoto Encyclopaedia of Genes and Genomes (KEGG) pathway analyses of the differentially expressed genes enriched cell division and cell cycle respectively. MiR-33a overexpression decreased the rate of preadipocyte proliferation. Synchronously, the mRNA and protein expression levels of proliferation-related marker genes, including cyclin B1 (CCNB1) and proliferating cell nuclear antigen (PCNA), were decreased. In contrast, inhibiting miR-33a increased the rate of preadipocyte proliferation, and expression levels of CCNB1 and PCNA. Furthermore, based on luciferase reporter assays, miR-33a targeted directly cyclin-dependent kinase 6 (CDK6)-3ʹUTR and inhibited CDK6 protein expression. Interestingly, the silencing of CDK6 inhibited bovine preadipocyte proliferation and proliferation-related genes. Therefore, miR-33a inhibits the proliferation of bovine preadipocytes. CDK6 is the target gene of miR-33a and may be involved in the effects of miR-33a on bovine preadipocyte proliferation. |
format | Online Article Text |
id | pubmed-8043176 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-80431762021-04-21 MiR-33a plays an crucial role in the proliferation of bovine preadipocytes Zhang, Wenzhen Wang, Li Raza, Sayed Haidar Abbas Wang, Xiaoyu Wang, Guohu Liang, Chengcheng Cheng, Gong Li, Bingzhi Zan, Linsen Adipocyte Research Paper Preadipocyte proliferation is a critical and precisely orchestrated procedure in adipogenesis, which is highly regulated by microRNAs (miRNAs). A previous study identified that the expression of miR-33a is different in intramuscular fat (IMF) tissues from steers and bulls. In the present study, miR-33a was overexpressed in bovine preadipocytes, and a total of 781 differentialy expressed genes were found, including 348 upregulated and 433 downregulated genes. Gene Ontology and Kyoto Encyclopaedia of Genes and Genomes (KEGG) pathway analyses of the differentially expressed genes enriched cell division and cell cycle respectively. MiR-33a overexpression decreased the rate of preadipocyte proliferation. Synchronously, the mRNA and protein expression levels of proliferation-related marker genes, including cyclin B1 (CCNB1) and proliferating cell nuclear antigen (PCNA), were decreased. In contrast, inhibiting miR-33a increased the rate of preadipocyte proliferation, and expression levels of CCNB1 and PCNA. Furthermore, based on luciferase reporter assays, miR-33a targeted directly cyclin-dependent kinase 6 (CDK6)-3ʹUTR and inhibited CDK6 protein expression. Interestingly, the silencing of CDK6 inhibited bovine preadipocyte proliferation and proliferation-related genes. Therefore, miR-33a inhibits the proliferation of bovine preadipocytes. CDK6 is the target gene of miR-33a and may be involved in the effects of miR-33a on bovine preadipocyte proliferation. Taylor & Francis 2021-04-12 /pmc/articles/PMC8043176/ /pubmed/33840361 http://dx.doi.org/10.1080/21623945.2021.1908655 Text en © 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Paper Zhang, Wenzhen Wang, Li Raza, Sayed Haidar Abbas Wang, Xiaoyu Wang, Guohu Liang, Chengcheng Cheng, Gong Li, Bingzhi Zan, Linsen MiR-33a plays an crucial role in the proliferation of bovine preadipocytes |
title | MiR-33a plays an crucial role in the proliferation of bovine preadipocytes |
title_full | MiR-33a plays an crucial role in the proliferation of bovine preadipocytes |
title_fullStr | MiR-33a plays an crucial role in the proliferation of bovine preadipocytes |
title_full_unstemmed | MiR-33a plays an crucial role in the proliferation of bovine preadipocytes |
title_short | MiR-33a plays an crucial role in the proliferation of bovine preadipocytes |
title_sort | mir-33a plays an crucial role in the proliferation of bovine preadipocytes |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8043176/ https://www.ncbi.nlm.nih.gov/pubmed/33840361 http://dx.doi.org/10.1080/21623945.2021.1908655 |
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