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MiR-33a plays an crucial role in the proliferation of bovine preadipocytes

Preadipocyte proliferation is a critical and precisely orchestrated procedure in adipogenesis, which is highly regulated by microRNAs (miRNAs). A previous study identified that the expression of miR-33a is different in intramuscular fat (IMF) tissues from steers and bulls. In the present study, miR-...

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Autores principales: Zhang, Wenzhen, Wang, Li, Raza, Sayed Haidar Abbas, Wang, Xiaoyu, Wang, Guohu, Liang, Chengcheng, Cheng, Gong, Li, Bingzhi, Zan, Linsen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8043176/
https://www.ncbi.nlm.nih.gov/pubmed/33840361
http://dx.doi.org/10.1080/21623945.2021.1908655
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author Zhang, Wenzhen
Wang, Li
Raza, Sayed Haidar Abbas
Wang, Xiaoyu
Wang, Guohu
Liang, Chengcheng
Cheng, Gong
Li, Bingzhi
Zan, Linsen
author_facet Zhang, Wenzhen
Wang, Li
Raza, Sayed Haidar Abbas
Wang, Xiaoyu
Wang, Guohu
Liang, Chengcheng
Cheng, Gong
Li, Bingzhi
Zan, Linsen
author_sort Zhang, Wenzhen
collection PubMed
description Preadipocyte proliferation is a critical and precisely orchestrated procedure in adipogenesis, which is highly regulated by microRNAs (miRNAs). A previous study identified that the expression of miR-33a is different in intramuscular fat (IMF) tissues from steers and bulls. In the present study, miR-33a was overexpressed in bovine preadipocytes, and a total of 781 differentialy expressed genes were found, including 348 upregulated and 433 downregulated genes. Gene Ontology and Kyoto Encyclopaedia of Genes and Genomes (KEGG) pathway analyses of the differentially expressed genes enriched cell division and cell cycle respectively. MiR-33a overexpression decreased the rate of preadipocyte proliferation. Synchronously, the mRNA and protein expression levels of proliferation-related marker genes, including cyclin B1 (CCNB1) and proliferating cell nuclear antigen (PCNA), were decreased. In contrast, inhibiting miR-33a increased the rate of preadipocyte proliferation, and expression levels of CCNB1 and PCNA. Furthermore, based on luciferase reporter assays, miR-33a targeted directly cyclin-dependent kinase 6 (CDK6)-3ʹUTR and inhibited CDK6 protein expression. Interestingly, the silencing of CDK6 inhibited bovine preadipocyte proliferation and proliferation-related genes. Therefore, miR-33a inhibits the proliferation of bovine preadipocytes. CDK6 is the target gene of miR-33a and may be involved in the effects of miR-33a on bovine preadipocyte proliferation.
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spelling pubmed-80431762021-04-21 MiR-33a plays an crucial role in the proliferation of bovine preadipocytes Zhang, Wenzhen Wang, Li Raza, Sayed Haidar Abbas Wang, Xiaoyu Wang, Guohu Liang, Chengcheng Cheng, Gong Li, Bingzhi Zan, Linsen Adipocyte Research Paper Preadipocyte proliferation is a critical and precisely orchestrated procedure in adipogenesis, which is highly regulated by microRNAs (miRNAs). A previous study identified that the expression of miR-33a is different in intramuscular fat (IMF) tissues from steers and bulls. In the present study, miR-33a was overexpressed in bovine preadipocytes, and a total of 781 differentialy expressed genes were found, including 348 upregulated and 433 downregulated genes. Gene Ontology and Kyoto Encyclopaedia of Genes and Genomes (KEGG) pathway analyses of the differentially expressed genes enriched cell division and cell cycle respectively. MiR-33a overexpression decreased the rate of preadipocyte proliferation. Synchronously, the mRNA and protein expression levels of proliferation-related marker genes, including cyclin B1 (CCNB1) and proliferating cell nuclear antigen (PCNA), were decreased. In contrast, inhibiting miR-33a increased the rate of preadipocyte proliferation, and expression levels of CCNB1 and PCNA. Furthermore, based on luciferase reporter assays, miR-33a targeted directly cyclin-dependent kinase 6 (CDK6)-3ʹUTR and inhibited CDK6 protein expression. Interestingly, the silencing of CDK6 inhibited bovine preadipocyte proliferation and proliferation-related genes. Therefore, miR-33a inhibits the proliferation of bovine preadipocytes. CDK6 is the target gene of miR-33a and may be involved in the effects of miR-33a on bovine preadipocyte proliferation. Taylor & Francis 2021-04-12 /pmc/articles/PMC8043176/ /pubmed/33840361 http://dx.doi.org/10.1080/21623945.2021.1908655 Text en © 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Zhang, Wenzhen
Wang, Li
Raza, Sayed Haidar Abbas
Wang, Xiaoyu
Wang, Guohu
Liang, Chengcheng
Cheng, Gong
Li, Bingzhi
Zan, Linsen
MiR-33a plays an crucial role in the proliferation of bovine preadipocytes
title MiR-33a plays an crucial role in the proliferation of bovine preadipocytes
title_full MiR-33a plays an crucial role in the proliferation of bovine preadipocytes
title_fullStr MiR-33a plays an crucial role in the proliferation of bovine preadipocytes
title_full_unstemmed MiR-33a plays an crucial role in the proliferation of bovine preadipocytes
title_short MiR-33a plays an crucial role in the proliferation of bovine preadipocytes
title_sort mir-33a plays an crucial role in the proliferation of bovine preadipocytes
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8043176/
https://www.ncbi.nlm.nih.gov/pubmed/33840361
http://dx.doi.org/10.1080/21623945.2021.1908655
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