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Expression of cornulin in tongue squamous cell carcinoma

The aim of the study is to identify cornulin (CRNN) protein expression associated with advancement of tongue squamous cell carcinoma (TSCC). A comparison of addictive (containing potential carcinogens) versus non-addiction causative agents was expected to allow detection of differences in CRNN expre...

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Autores principales: Saleem, Saira, Aleem, Iffat, Atiq, Aribah, Tariq, Sahrish, Babar, Amna, Bakar, Muhammad Abu, Syed, Madiha, Maruf, Maheen, Mahmood, Mohammad Tariq, Zeshan, Muhammad, Tahseen, Muhammad, Hussain, Raza, Loya, Asif, Sutton, Chris
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cancer Intelligence 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8043688/
https://www.ncbi.nlm.nih.gov/pubmed/33889206
http://dx.doi.org/10.3332/ecancer.2021.1197
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author Saleem, Saira
Aleem, Iffat
Atiq, Aribah
Tariq, Sahrish
Babar, Amna
Bakar, Muhammad Abu
Syed, Madiha
Maruf, Maheen
Mahmood, Mohammad Tariq
Zeshan, Muhammad
Tahseen, Muhammad
Hussain, Raza
Loya, Asif
Sutton, Chris
author_facet Saleem, Saira
Aleem, Iffat
Atiq, Aribah
Tariq, Sahrish
Babar, Amna
Bakar, Muhammad Abu
Syed, Madiha
Maruf, Maheen
Mahmood, Mohammad Tariq
Zeshan, Muhammad
Tahseen, Muhammad
Hussain, Raza
Loya, Asif
Sutton, Chris
author_sort Saleem, Saira
collection PubMed
description The aim of the study is to identify cornulin (CRNN) protein expression associated with advancement of tongue squamous cell carcinoma (TSCC). A comparison of addictive (containing potential carcinogens) versus non-addiction causative agents was expected to allow detection of differences in CRNN expression associated with TSCC. Bespoke tissue microarrays (TMAs) were prepared and immunohistochemistry (IHC) performed to determine the changes in CRNN expression in epithelial cells of node-negative (pN-), node-positive (pN+) TSCC and non-cancer patients’ oral tissues. TMAs were validated by performing IHC on whole diagnostic tissues. Chi-square test or Fisher’s-exact tests were used to establish significant expression differences. Analogous analyses were performed for biomarkers previously associated with TSCC, namely collagen I alpha 2 (COL1A2) and decorin (DCN) to compare the significance of CRNN. Keratinisation and its level (low, extensive) were studied in relation to CRNN so that the extent of squamous differentiation could better be assessed. IHC immunoreactive score (IRS) clustered the patients based on weak/moderate (Low (IRS ≤ +3)) or strong (High (IRS ≥ +4)) expression groups. A low expression was observed in a larger number of patients in control proteins COL1A2 (77.3%), DCN (87.5%) and target protein CRNN (52.3%), respectively. Low CRNN expression was observed in TSCC where nodes were involved (pN+: mean 1.4 ± 2.1) (p = 0.248). Keratinisation (%) was low (0% ≤ 50%) in 42.2% and extensive (1% ≥ 50.0%) in 57.8% patients. In conclusion, our study suggested that Low CRNN expression was associated with grade and lymph node metastasis in TSCC. CRNN expression is independent of addiction, however potentially carcinogenic addictive substances might be aiding in the disease progression.
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spelling pubmed-80436882021-04-21 Expression of cornulin in tongue squamous cell carcinoma Saleem, Saira Aleem, Iffat Atiq, Aribah Tariq, Sahrish Babar, Amna Bakar, Muhammad Abu Syed, Madiha Maruf, Maheen Mahmood, Mohammad Tariq Zeshan, Muhammad Tahseen, Muhammad Hussain, Raza Loya, Asif Sutton, Chris Ecancermedicalscience Research The aim of the study is to identify cornulin (CRNN) protein expression associated with advancement of tongue squamous cell carcinoma (TSCC). A comparison of addictive (containing potential carcinogens) versus non-addiction causative agents was expected to allow detection of differences in CRNN expression associated with TSCC. Bespoke tissue microarrays (TMAs) were prepared and immunohistochemistry (IHC) performed to determine the changes in CRNN expression in epithelial cells of node-negative (pN-), node-positive (pN+) TSCC and non-cancer patients’ oral tissues. TMAs were validated by performing IHC on whole diagnostic tissues. Chi-square test or Fisher’s-exact tests were used to establish significant expression differences. Analogous analyses were performed for biomarkers previously associated with TSCC, namely collagen I alpha 2 (COL1A2) and decorin (DCN) to compare the significance of CRNN. Keratinisation and its level (low, extensive) were studied in relation to CRNN so that the extent of squamous differentiation could better be assessed. IHC immunoreactive score (IRS) clustered the patients based on weak/moderate (Low (IRS ≤ +3)) or strong (High (IRS ≥ +4)) expression groups. A low expression was observed in a larger number of patients in control proteins COL1A2 (77.3%), DCN (87.5%) and target protein CRNN (52.3%), respectively. Low CRNN expression was observed in TSCC where nodes were involved (pN+: mean 1.4 ± 2.1) (p = 0.248). Keratinisation (%) was low (0% ≤ 50%) in 42.2% and extensive (1% ≥ 50.0%) in 57.8% patients. In conclusion, our study suggested that Low CRNN expression was associated with grade and lymph node metastasis in TSCC. CRNN expression is independent of addiction, however potentially carcinogenic addictive substances might be aiding in the disease progression. Cancer Intelligence 2021-03-04 /pmc/articles/PMC8043688/ /pubmed/33889206 http://dx.doi.org/10.3332/ecancer.2021.1197 Text en © the authors; licensee ecancermedicalscience. https://creativecommons.org/licenses/by/3.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0 (https://creativecommons.org/licenses/by/3.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Saleem, Saira
Aleem, Iffat
Atiq, Aribah
Tariq, Sahrish
Babar, Amna
Bakar, Muhammad Abu
Syed, Madiha
Maruf, Maheen
Mahmood, Mohammad Tariq
Zeshan, Muhammad
Tahseen, Muhammad
Hussain, Raza
Loya, Asif
Sutton, Chris
Expression of cornulin in tongue squamous cell carcinoma
title Expression of cornulin in tongue squamous cell carcinoma
title_full Expression of cornulin in tongue squamous cell carcinoma
title_fullStr Expression of cornulin in tongue squamous cell carcinoma
title_full_unstemmed Expression of cornulin in tongue squamous cell carcinoma
title_short Expression of cornulin in tongue squamous cell carcinoma
title_sort expression of cornulin in tongue squamous cell carcinoma
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8043688/
https://www.ncbi.nlm.nih.gov/pubmed/33889206
http://dx.doi.org/10.3332/ecancer.2021.1197
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