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Clinical characteristics and molecular detection of Bordetella pertussis in hospitalized children with a clinical diagnosis of whooping cough in Peru

BACKGROUND AND OBJECTIVES: Pertussis is an infectious disease caused by the Gram-negative bacterium Bordetella pertussis. In Peru, actual public health programs indicate that vaccination against B. pertussis must be mandatory and generalized, besides all detected cases must be reported. The objectiv...

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Autores principales: del Valle-Mendoza, Juana, del Valle-Vargas, Cristina, Aquino-Ortega, Ronald, del Valle, Luis J., Cieza-Mora, Erico, Silva-Caso, Wilmer, Bazán-Mayra, Jorge, Zavaleta-Gavidia, Victor, Aguilar-Luis, Miguel Angel, Cornejo-Pacherres, Hernán, Martins-Luna, Johanna, Cornejo-Tapia, Angela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Tehran University of Medical Sciences 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8043828/
https://www.ncbi.nlm.nih.gov/pubmed/33889359
http://dx.doi.org/10.18502/ijm.v13i1.5488
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author del Valle-Mendoza, Juana
del Valle-Vargas, Cristina
Aquino-Ortega, Ronald
del Valle, Luis J.
Cieza-Mora, Erico
Silva-Caso, Wilmer
Bazán-Mayra, Jorge
Zavaleta-Gavidia, Victor
Aguilar-Luis, Miguel Angel
Cornejo-Pacherres, Hernán
Martins-Luna, Johanna
Cornejo-Tapia, Angela
author_facet del Valle-Mendoza, Juana
del Valle-Vargas, Cristina
Aquino-Ortega, Ronald
del Valle, Luis J.
Cieza-Mora, Erico
Silva-Caso, Wilmer
Bazán-Mayra, Jorge
Zavaleta-Gavidia, Victor
Aguilar-Luis, Miguel Angel
Cornejo-Pacherres, Hernán
Martins-Luna, Johanna
Cornejo-Tapia, Angela
author_sort del Valle-Mendoza, Juana
collection PubMed
description BACKGROUND AND OBJECTIVES: Pertussis is an infectious disease caused by the Gram-negative bacterium Bordetella pertussis. In Peru, actual public health programs indicate that vaccination against B. pertussis must be mandatory and generalized, besides all detected cases must be reported. The objective of this study was to determine the prevalence of B. pertussis among children under five years of age with a presumptive diagnosis of whopping cough in Cajamarca, a region located in northern Peru. MATERIALS AND METHODS: The population of this cross-sectional study were children under 5 years old hospitalized as presumptive cases of pertussis during December 2017 to December 2018. The nasopharyngeal samples were analyzed by real-time PCR for the detection of B. pertussis. RESULTS: B. pertussis was identified as PCR + in 42.3% of our sample (33/78). The clinical presentation that was observed most frequently includes paroxysmal coughing (97%), difficulty breathing (69.7%), cyanosis (72.7%) and post-tussive emesis (60.6%). Additionally, pneumonia was the most observed complication (33.3%). Four of the patients with PCR+ for B. pertussis presented only lymphocytosis, five only leukocytosis, two patients with decreased leukocytosis and lymphocytes and only one patient with leukopenia and relative lymphocytosis. There was a percentage of 84.8% of unvaccinated children in the PCR+ group. Finally, the mother was the most frequent symptom carrier (18.2%). CONCLUSION: In conclusion, in the studied population there is a high rate of PCR+ cases for B. pertussis. Laboratory values may show leukopenia or lymphopenia in patients with pertussis. It is necessary to use appropriate laboratory diagnostic tests in all infants with respiratory symptoms for B. pertussis. Since, the clinical diagnosis overestimates the diagnosis of pertussis.
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spelling pubmed-80438282021-04-21 Clinical characteristics and molecular detection of Bordetella pertussis in hospitalized children with a clinical diagnosis of whooping cough in Peru del Valle-Mendoza, Juana del Valle-Vargas, Cristina Aquino-Ortega, Ronald del Valle, Luis J. Cieza-Mora, Erico Silva-Caso, Wilmer Bazán-Mayra, Jorge Zavaleta-Gavidia, Victor Aguilar-Luis, Miguel Angel Cornejo-Pacherres, Hernán Martins-Luna, Johanna Cornejo-Tapia, Angela Iran J Microbiol Original Article BACKGROUND AND OBJECTIVES: Pertussis is an infectious disease caused by the Gram-negative bacterium Bordetella pertussis. In Peru, actual public health programs indicate that vaccination against B. pertussis must be mandatory and generalized, besides all detected cases must be reported. The objective of this study was to determine the prevalence of B. pertussis among children under five years of age with a presumptive diagnosis of whopping cough in Cajamarca, a region located in northern Peru. MATERIALS AND METHODS: The population of this cross-sectional study were children under 5 years old hospitalized as presumptive cases of pertussis during December 2017 to December 2018. The nasopharyngeal samples were analyzed by real-time PCR for the detection of B. pertussis. RESULTS: B. pertussis was identified as PCR + in 42.3% of our sample (33/78). The clinical presentation that was observed most frequently includes paroxysmal coughing (97%), difficulty breathing (69.7%), cyanosis (72.7%) and post-tussive emesis (60.6%). Additionally, pneumonia was the most observed complication (33.3%). Four of the patients with PCR+ for B. pertussis presented only lymphocytosis, five only leukocytosis, two patients with decreased leukocytosis and lymphocytes and only one patient with leukopenia and relative lymphocytosis. There was a percentage of 84.8% of unvaccinated children in the PCR+ group. Finally, the mother was the most frequent symptom carrier (18.2%). CONCLUSION: In conclusion, in the studied population there is a high rate of PCR+ cases for B. pertussis. Laboratory values may show leukopenia or lymphopenia in patients with pertussis. It is necessary to use appropriate laboratory diagnostic tests in all infants with respiratory symptoms for B. pertussis. Since, the clinical diagnosis overestimates the diagnosis of pertussis. Tehran University of Medical Sciences 2021-02 /pmc/articles/PMC8043828/ /pubmed/33889359 http://dx.doi.org/10.18502/ijm.v13i1.5488 Text en Copyright © 2021 The Authors. Published by Tehran University of Medical Sciences https://creativecommons.org/licenses/by-nc/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International license (https://creativecommons.org/licenses/by-nc/4.0/). Non-commercial uses of the work are permitted, provided the original work is properly cited.
spellingShingle Original Article
del Valle-Mendoza, Juana
del Valle-Vargas, Cristina
Aquino-Ortega, Ronald
del Valle, Luis J.
Cieza-Mora, Erico
Silva-Caso, Wilmer
Bazán-Mayra, Jorge
Zavaleta-Gavidia, Victor
Aguilar-Luis, Miguel Angel
Cornejo-Pacherres, Hernán
Martins-Luna, Johanna
Cornejo-Tapia, Angela
Clinical characteristics and molecular detection of Bordetella pertussis in hospitalized children with a clinical diagnosis of whooping cough in Peru
title Clinical characteristics and molecular detection of Bordetella pertussis in hospitalized children with a clinical diagnosis of whooping cough in Peru
title_full Clinical characteristics and molecular detection of Bordetella pertussis in hospitalized children with a clinical diagnosis of whooping cough in Peru
title_fullStr Clinical characteristics and molecular detection of Bordetella pertussis in hospitalized children with a clinical diagnosis of whooping cough in Peru
title_full_unstemmed Clinical characteristics and molecular detection of Bordetella pertussis in hospitalized children with a clinical diagnosis of whooping cough in Peru
title_short Clinical characteristics and molecular detection of Bordetella pertussis in hospitalized children with a clinical diagnosis of whooping cough in Peru
title_sort clinical characteristics and molecular detection of bordetella pertussis in hospitalized children with a clinical diagnosis of whooping cough in peru
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8043828/
https://www.ncbi.nlm.nih.gov/pubmed/33889359
http://dx.doi.org/10.18502/ijm.v13i1.5488
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