A tandem giant magnetoresistance assay for one-shot quantification of clinically relevant concentrations of N-terminal pro-B-type natriuretic peptide in human blood

We report a microfluidic sandwich immunoassay constructed around a dual-giant magnetoresistance (GMR) sensor array to quantify the heart failure biomarker NT-proBNP in human plasma at the clinically relevant concentration levels between 15 pg/mL and 40 ng/mL. The broad dynamic range was achieved by...

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Autores principales: Meng, Fanda, Huo, Weisong, Lian, Jie, Zhang, Lei, Shi, Xizeng, Jesorka, Aldo, Gao, Yunhua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2021
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8043887/
https://www.ncbi.nlm.nih.gov/pubmed/33624128
http://dx.doi.org/10.1007/s00216-021-03227-5
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author Meng, Fanda
Huo, Weisong
Lian, Jie
Zhang, Lei
Shi, Xizeng
Jesorka, Aldo
Gao, Yunhua
author_facet Meng, Fanda
Huo, Weisong
Lian, Jie
Zhang, Lei
Shi, Xizeng
Jesorka, Aldo
Gao, Yunhua
author_sort Meng, Fanda
collection PubMed
description We report a microfluidic sandwich immunoassay constructed around a dual-giant magnetoresistance (GMR) sensor array to quantify the heart failure biomarker NT-proBNP in human plasma at the clinically relevant concentration levels between 15 pg/mL and 40 ng/mL. The broad dynamic range was achieved by differential coating of two identical GMR sensors operated in tandem, and combining two standard curves. The detection limit was determined as 5 pg/mL. The assay, involving 53 plasma samples from patients with different cardiovascular diseases, was validated against the Roche Cobas e411 analyzer. The salient features of this system are its wide concentration range, low detection limit, small sample volume requirement (50 μL), and the need for a short measurement time of 15 min, making it a prospective candidate for practical use in point of care analysis.
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spelling pubmed-80438872021-04-27 A tandem giant magnetoresistance assay for one-shot quantification of clinically relevant concentrations of N-terminal pro-B-type natriuretic peptide in human blood Meng, Fanda Huo, Weisong Lian, Jie Zhang, Lei Shi, Xizeng Jesorka, Aldo Gao, Yunhua Anal Bioanal Chem Research Paper We report a microfluidic sandwich immunoassay constructed around a dual-giant magnetoresistance (GMR) sensor array to quantify the heart failure biomarker NT-proBNP in human plasma at the clinically relevant concentration levels between 15 pg/mL and 40 ng/mL. The broad dynamic range was achieved by differential coating of two identical GMR sensors operated in tandem, and combining two standard curves. The detection limit was determined as 5 pg/mL. The assay, involving 53 plasma samples from patients with different cardiovascular diseases, was validated against the Roche Cobas e411 analyzer. The salient features of this system are its wide concentration range, low detection limit, small sample volume requirement (50 μL), and the need for a short measurement time of 15 min, making it a prospective candidate for practical use in point of care analysis. Springer Berlin Heidelberg 2021-02-23 2021 /pmc/articles/PMC8043887/ /pubmed/33624128 http://dx.doi.org/10.1007/s00216-021-03227-5 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Paper
Meng, Fanda
Huo, Weisong
Lian, Jie
Zhang, Lei
Shi, Xizeng
Jesorka, Aldo
Gao, Yunhua
A tandem giant magnetoresistance assay for one-shot quantification of clinically relevant concentrations of N-terminal pro-B-type natriuretic peptide in human blood
title A tandem giant magnetoresistance assay for one-shot quantification of clinically relevant concentrations of N-terminal pro-B-type natriuretic peptide in human blood
title_full A tandem giant magnetoresistance assay for one-shot quantification of clinically relevant concentrations of N-terminal pro-B-type natriuretic peptide in human blood
title_fullStr A tandem giant magnetoresistance assay for one-shot quantification of clinically relevant concentrations of N-terminal pro-B-type natriuretic peptide in human blood
title_full_unstemmed A tandem giant magnetoresistance assay for one-shot quantification of clinically relevant concentrations of N-terminal pro-B-type natriuretic peptide in human blood
title_short A tandem giant magnetoresistance assay for one-shot quantification of clinically relevant concentrations of N-terminal pro-B-type natriuretic peptide in human blood
title_sort tandem giant magnetoresistance assay for one-shot quantification of clinically relevant concentrations of n-terminal pro-b-type natriuretic peptide in human blood
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8043887/
https://www.ncbi.nlm.nih.gov/pubmed/33624128
http://dx.doi.org/10.1007/s00216-021-03227-5
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