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Mechanical Reperfusion Following Prolonged Global Cerebral Ischemia Attenuates Brain Injury
Previous experiments demonstrated improved outcome following prolonged cerebral ischemia given controlled brain reperfusion using extracorporeal circulation. The current study further investigates this. Young adult pigs were exposed to 30 min of global normothermic cerebral ischemia, achieved throug...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer US
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8043930/ https://www.ncbi.nlm.nih.gov/pubmed/32681452 http://dx.doi.org/10.1007/s12265-020-10058-9 |
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author | Lindblom, Rickard P. F. Tovedal, Thomas Norlin, Bo Hillered, Lars Englund, Elisabet Thelin, Stefan |
author_facet | Lindblom, Rickard P. F. Tovedal, Thomas Norlin, Bo Hillered, Lars Englund, Elisabet Thelin, Stefan |
author_sort | Lindblom, Rickard P. F. |
collection | PubMed |
description | Previous experiments demonstrated improved outcome following prolonged cerebral ischemia given controlled brain reperfusion using extracorporeal circulation. The current study further investigates this. Young adult pigs were exposed to 30 min of global normothermic cerebral ischemia, achieved through intrathoracic clamping of cerebral arteries, followed by 20 min of isolated mechanical brain reperfusion. Leukocyte-filtered blood was delivered by a roller-pump at fixed pressure and flow. One experimental group additionally had a custom-made buffer solution delivered at 1:8 ratio with the blood. Hemodynamics including intracranial pressure were monitored. Blood gases were from peripheral arteries and the sagittal sinus, and intraparenchymal brain microdialysis was performed. The brains were examined by a neuropathologist. The group with the added buffer showed lower intracranial pressure as well as decreased intraparenchymal glycerol and less signs of excitotoxicity and ischemia, although histology revealed similar degrees of injury. A customized mechanical reperfusion improves multiple parameters after prolonged normothermic global cerebral ischemia. [Figure: see text] ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s12265-020-10058-9) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-8043930 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Springer US |
record_format | MEDLINE/PubMed |
spelling | pubmed-80439302021-04-27 Mechanical Reperfusion Following Prolonged Global Cerebral Ischemia Attenuates Brain Injury Lindblom, Rickard P. F. Tovedal, Thomas Norlin, Bo Hillered, Lars Englund, Elisabet Thelin, Stefan J Cardiovasc Transl Res Original Article Previous experiments demonstrated improved outcome following prolonged cerebral ischemia given controlled brain reperfusion using extracorporeal circulation. The current study further investigates this. Young adult pigs were exposed to 30 min of global normothermic cerebral ischemia, achieved through intrathoracic clamping of cerebral arteries, followed by 20 min of isolated mechanical brain reperfusion. Leukocyte-filtered blood was delivered by a roller-pump at fixed pressure and flow. One experimental group additionally had a custom-made buffer solution delivered at 1:8 ratio with the blood. Hemodynamics including intracranial pressure were monitored. Blood gases were from peripheral arteries and the sagittal sinus, and intraparenchymal brain microdialysis was performed. The brains were examined by a neuropathologist. The group with the added buffer showed lower intracranial pressure as well as decreased intraparenchymal glycerol and less signs of excitotoxicity and ischemia, although histology revealed similar degrees of injury. A customized mechanical reperfusion improves multiple parameters after prolonged normothermic global cerebral ischemia. [Figure: see text] ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s12265-020-10058-9) contains supplementary material, which is available to authorized users. Springer US 2020-07-17 2021 /pmc/articles/PMC8043930/ /pubmed/32681452 http://dx.doi.org/10.1007/s12265-020-10058-9 Text en © The Author(s) 2020 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Original Article Lindblom, Rickard P. F. Tovedal, Thomas Norlin, Bo Hillered, Lars Englund, Elisabet Thelin, Stefan Mechanical Reperfusion Following Prolonged Global Cerebral Ischemia Attenuates Brain Injury |
title | Mechanical Reperfusion Following Prolonged Global Cerebral Ischemia Attenuates Brain Injury |
title_full | Mechanical Reperfusion Following Prolonged Global Cerebral Ischemia Attenuates Brain Injury |
title_fullStr | Mechanical Reperfusion Following Prolonged Global Cerebral Ischemia Attenuates Brain Injury |
title_full_unstemmed | Mechanical Reperfusion Following Prolonged Global Cerebral Ischemia Attenuates Brain Injury |
title_short | Mechanical Reperfusion Following Prolonged Global Cerebral Ischemia Attenuates Brain Injury |
title_sort | mechanical reperfusion following prolonged global cerebral ischemia attenuates brain injury |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8043930/ https://www.ncbi.nlm.nih.gov/pubmed/32681452 http://dx.doi.org/10.1007/s12265-020-10058-9 |
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