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Synthesizing Electrodes Into Electrochemical Sensor Systems
Electrochemical sensors that can determine single/multiple analytes remain a key challenge in miniaturized analytical systems and devices. In this study, we present in situ synthesis and modification of gold nanodendrite electrodes to create an electrochemical system for the analysis of hydrogen per...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8044375/ https://www.ncbi.nlm.nih.gov/pubmed/33869143 http://dx.doi.org/10.3389/fchem.2021.641674 |
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author | Mourzina, Yulia G. Ermolenko, Yuri E. Offenhäusser, Andreas |
author_facet | Mourzina, Yulia G. Ermolenko, Yuri E. Offenhäusser, Andreas |
author_sort | Mourzina, Yulia G. |
collection | PubMed |
description | Electrochemical sensors that can determine single/multiple analytes remain a key challenge in miniaturized analytical systems and devices. In this study, we present in situ synthesis and modification of gold nanodendrite electrodes to create an electrochemical system for the analysis of hydrogen peroxide. The sensor system consisted of the reference and counter electrodes as well as the working electrode. Electrochemical reduction of graphene oxide, ErGO, on the thin-film gold and gold nanodendrite working electrodes was used to achieve an efficient sensor interface for the adsorption of a biomimetic electrocatalytic sensor material, Mn(III) meso-tetra(N-methyl-4-pyridyl) porphyrin complex, with as high as 10(–10) mol cm(−2) surface coverage. The sensor system demonstrated a detection limit of 0.3 µM H(2)O(2) in the presence of oxygen. Electrochemical determination of hydrogen peroxide in plant material in the concentration range from 0.09 to 0.4 µmol (gFW)(−1) using the electrochemical sensor system was shown as well as in vivo real-time monitoring of the hydrogen peroxide dynamics as a sign of abiotic stress (intense sunlight). Results of the electrochemical determination were in good agreement with the results of biochemical analysis with the spectrophotometric detection. We anticipate that this method can be extended for the synthesis and integration of multisensor arrays in analytical microsystems and devices for the quantification and real-time in vivo monitoring of other analytes and biomarkers. |
format | Online Article Text |
id | pubmed-8044375 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-80443752021-04-15 Synthesizing Electrodes Into Electrochemical Sensor Systems Mourzina, Yulia G. Ermolenko, Yuri E. Offenhäusser, Andreas Front Chem Chemistry Electrochemical sensors that can determine single/multiple analytes remain a key challenge in miniaturized analytical systems and devices. In this study, we present in situ synthesis and modification of gold nanodendrite electrodes to create an electrochemical system for the analysis of hydrogen peroxide. The sensor system consisted of the reference and counter electrodes as well as the working electrode. Electrochemical reduction of graphene oxide, ErGO, on the thin-film gold and gold nanodendrite working electrodes was used to achieve an efficient sensor interface for the adsorption of a biomimetic electrocatalytic sensor material, Mn(III) meso-tetra(N-methyl-4-pyridyl) porphyrin complex, with as high as 10(–10) mol cm(−2) surface coverage. The sensor system demonstrated a detection limit of 0.3 µM H(2)O(2) in the presence of oxygen. Electrochemical determination of hydrogen peroxide in plant material in the concentration range from 0.09 to 0.4 µmol (gFW)(−1) using the electrochemical sensor system was shown as well as in vivo real-time monitoring of the hydrogen peroxide dynamics as a sign of abiotic stress (intense sunlight). Results of the electrochemical determination were in good agreement with the results of biochemical analysis with the spectrophotometric detection. We anticipate that this method can be extended for the synthesis and integration of multisensor arrays in analytical microsystems and devices for the quantification and real-time in vivo monitoring of other analytes and biomarkers. Frontiers Media S.A. 2021-03-31 /pmc/articles/PMC8044375/ /pubmed/33869143 http://dx.doi.org/10.3389/fchem.2021.641674 Text en Copyright © 2021 Mourzina, Ermolenko and Offenhäusser. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Chemistry Mourzina, Yulia G. Ermolenko, Yuri E. Offenhäusser, Andreas Synthesizing Electrodes Into Electrochemical Sensor Systems |
title | Synthesizing Electrodes Into Electrochemical Sensor Systems |
title_full | Synthesizing Electrodes Into Electrochemical Sensor Systems |
title_fullStr | Synthesizing Electrodes Into Electrochemical Sensor Systems |
title_full_unstemmed | Synthesizing Electrodes Into Electrochemical Sensor Systems |
title_short | Synthesizing Electrodes Into Electrochemical Sensor Systems |
title_sort | synthesizing electrodes into electrochemical sensor systems |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8044375/ https://www.ncbi.nlm.nih.gov/pubmed/33869143 http://dx.doi.org/10.3389/fchem.2021.641674 |
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