Isolation of stromal vascular fraction cell suspensions from mouse and human adipose tissues for downstream applications

This protocol outlines a reliable and versatile approach to isolate stromal vascular fraction cells from different adipose tissues across human and mouse species. A number of downstream applications can then be performed to gain an appreciation of the functional activity of unique adipose tissue-res...

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Detalles Bibliográficos
Autores principales: Hearnden, Robert, Sandhar, Balraj, Vyas, Vishal, Longhi, M. Paula
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8044717/
https://www.ncbi.nlm.nih.gov/pubmed/33870227
http://dx.doi.org/10.1016/j.xpro.2021.100422
Descripción
Sumario:This protocol outlines a reliable and versatile approach to isolate stromal vascular fraction cells from different adipose tissues across human and mouse species. A number of downstream applications can then be performed to gain an appreciation of the functional activity of unique adipose tissue-resident cell populations. For complete details on the use and execution of this protocol, please refer to Macdougall et al. (2018).

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