Isolation of stromal vascular fraction cell suspensions from mouse and human adipose tissues for downstream applications

This protocol outlines a reliable and versatile approach to isolate stromal vascular fraction cells from different adipose tissues across human and mouse species. A number of downstream applications can then be performed to gain an appreciation of the functional activity of unique adipose tissue-res...

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Detalles Bibliográficos
Autores principales: Hearnden, Robert, Sandhar, Balraj, Vyas, Vishal, Longhi, M. Paula
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8044717/
https://www.ncbi.nlm.nih.gov/pubmed/33870227
http://dx.doi.org/10.1016/j.xpro.2021.100422
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author Hearnden, Robert
Sandhar, Balraj
Vyas, Vishal
Longhi, M. Paula
author_facet Hearnden, Robert
Sandhar, Balraj
Vyas, Vishal
Longhi, M. Paula
author_sort Hearnden, Robert
collection PubMed
description This protocol outlines a reliable and versatile approach to isolate stromal vascular fraction cells from different adipose tissues across human and mouse species. A number of downstream applications can then be performed to gain an appreciation of the functional activity of unique adipose tissue-resident cell populations. For complete details on the use and execution of this protocol, please refer to Macdougall et al. (2018).
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spelling pubmed-80447172021-04-16 Isolation of stromal vascular fraction cell suspensions from mouse and human adipose tissues for downstream applications Hearnden, Robert Sandhar, Balraj Vyas, Vishal Longhi, M. Paula STAR Protoc Protocol This protocol outlines a reliable and versatile approach to isolate stromal vascular fraction cells from different adipose tissues across human and mouse species. A number of downstream applications can then be performed to gain an appreciation of the functional activity of unique adipose tissue-resident cell populations. For complete details on the use and execution of this protocol, please refer to Macdougall et al. (2018). Elsevier 2021-03-30 /pmc/articles/PMC8044717/ /pubmed/33870227 http://dx.doi.org/10.1016/j.xpro.2021.100422 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Hearnden, Robert
Sandhar, Balraj
Vyas, Vishal
Longhi, M. Paula
Isolation of stromal vascular fraction cell suspensions from mouse and human adipose tissues for downstream applications
title Isolation of stromal vascular fraction cell suspensions from mouse and human adipose tissues for downstream applications
title_full Isolation of stromal vascular fraction cell suspensions from mouse and human adipose tissues for downstream applications
title_fullStr Isolation of stromal vascular fraction cell suspensions from mouse and human adipose tissues for downstream applications
title_full_unstemmed Isolation of stromal vascular fraction cell suspensions from mouse and human adipose tissues for downstream applications
title_short Isolation of stromal vascular fraction cell suspensions from mouse and human adipose tissues for downstream applications
title_sort isolation of stromal vascular fraction cell suspensions from mouse and human adipose tissues for downstream applications
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8044717/
https://www.ncbi.nlm.nih.gov/pubmed/33870227
http://dx.doi.org/10.1016/j.xpro.2021.100422
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