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Optimized CRISPR/Cas9-mediated single nucleotide mutation in adherent cancer cell lines
CRISPR/Cas9 is an efficient, accurate, and optimizable genome-editing tool. Here, we present a modified CRISPR/Cas9 genome-editing protocol for single nucleotide mutation in adherent cell lines. The protocol was adapted to focus on ease of use and efficiency. The protocol here describes how to gener...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8044722/ https://www.ncbi.nlm.nih.gov/pubmed/33870225 http://dx.doi.org/10.1016/j.xpro.2021.100419 |