Protocol for establishing organoids from human ovarian cancer biopsies

Ovarian cancer (OC) is the most lethal gynecological cancer. Faithful research models are indispensable to the progression of understanding OC etiology and therapy. Here, we provide a detailed protocol for establishing organoid cultures from patient OC biopsies. The organoids reproduce primary tumor- and patient-specific characteristics including phenotypic properties and genomic aberrations and exhibit patient-dependent responsiveness to drugs. OC-derived organoids provide powerful tools to gain deep insight into the cancer’s pathobiology and to screen patient-tumor drug sensitivity to progress toward personalized medicine. For complete details on the use and execution of this protocol, please refer to Maenhoudt et al. (2020).