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Differential Cell Line Susceptibility to Crimean-Congo Hemorrhagic Fever Virus

Crimean-Congo hemorrhagic fever (CCHF) is a severe tick-borne viral disease of global concerns due to the increasing incidence and lack of effective treatments. The causative agent, CCHF virus (CCHFV), has been characterized for years; however, its tropism in cell lines of different host and tissue...

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Autores principales: Dai, Shiyu, Wu, Qiaoli, Wu, Xiaoli, Peng, Cheng, Liu, Jia, Tang, Shuang, Zhang, Tao, Deng, Fei, Shen, Shu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8044861/
https://www.ncbi.nlm.nih.gov/pubmed/33869079
http://dx.doi.org/10.3389/fcimb.2021.648077
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author Dai, Shiyu
Wu, Qiaoli
Wu, Xiaoli
Peng, Cheng
Liu, Jia
Tang, Shuang
Zhang, Tao
Deng, Fei
Shen, Shu
author_facet Dai, Shiyu
Wu, Qiaoli
Wu, Xiaoli
Peng, Cheng
Liu, Jia
Tang, Shuang
Zhang, Tao
Deng, Fei
Shen, Shu
author_sort Dai, Shiyu
collection PubMed
description Crimean-Congo hemorrhagic fever (CCHF) is a severe tick-borne viral disease of global concerns due to the increasing incidence and lack of effective treatments. The causative agent, CCHF virus (CCHFV), has been characterized for years; however, its tropism in cell lines of different host and tissue origins remains unclear. This study characterized the susceptibility of 16 human and 6 animal cell lines to CCHFV. Increased viral load and viral nucleoprotein expression, and productive CCHFV replication were detected in human vascular (HUVEC), renal (SW-13 and HEK-293), hepatic (Huh7), and cerebral (U-87 MG) cell lines, which were considered CCHFV-highly permissive cell lines. Renal cell lines derived from monkey and dog could also support CCHFV replication. This study evaluated the susceptibility of different cell lines to CCHFV and identified CCHFV-permissive cell lines. Our findings raise concerns regarding the use of cell lines in ex vivo studies of CCHFV and may have important implications for further fundamental research, which would promote understanding of CCHFV pathogenesis and transmission, as well as benefit designing strategies for disease prevention and control.
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spelling pubmed-80448612021-04-15 Differential Cell Line Susceptibility to Crimean-Congo Hemorrhagic Fever Virus Dai, Shiyu Wu, Qiaoli Wu, Xiaoli Peng, Cheng Liu, Jia Tang, Shuang Zhang, Tao Deng, Fei Shen, Shu Front Cell Infect Microbiol Cellular and Infection Microbiology Crimean-Congo hemorrhagic fever (CCHF) is a severe tick-borne viral disease of global concerns due to the increasing incidence and lack of effective treatments. The causative agent, CCHF virus (CCHFV), has been characterized for years; however, its tropism in cell lines of different host and tissue origins remains unclear. This study characterized the susceptibility of 16 human and 6 animal cell lines to CCHFV. Increased viral load and viral nucleoprotein expression, and productive CCHFV replication were detected in human vascular (HUVEC), renal (SW-13 and HEK-293), hepatic (Huh7), and cerebral (U-87 MG) cell lines, which were considered CCHFV-highly permissive cell lines. Renal cell lines derived from monkey and dog could also support CCHFV replication. This study evaluated the susceptibility of different cell lines to CCHFV and identified CCHFV-permissive cell lines. Our findings raise concerns regarding the use of cell lines in ex vivo studies of CCHFV and may have important implications for further fundamental research, which would promote understanding of CCHFV pathogenesis and transmission, as well as benefit designing strategies for disease prevention and control. Frontiers Media S.A. 2021-03-24 /pmc/articles/PMC8044861/ /pubmed/33869079 http://dx.doi.org/10.3389/fcimb.2021.648077 Text en Copyright © 2021 Dai, Wu, Wu, Peng, Liu, Tang, Zhang, Deng and Shen https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Dai, Shiyu
Wu, Qiaoli
Wu, Xiaoli
Peng, Cheng
Liu, Jia
Tang, Shuang
Zhang, Tao
Deng, Fei
Shen, Shu
Differential Cell Line Susceptibility to Crimean-Congo Hemorrhagic Fever Virus
title Differential Cell Line Susceptibility to Crimean-Congo Hemorrhagic Fever Virus
title_full Differential Cell Line Susceptibility to Crimean-Congo Hemorrhagic Fever Virus
title_fullStr Differential Cell Line Susceptibility to Crimean-Congo Hemorrhagic Fever Virus
title_full_unstemmed Differential Cell Line Susceptibility to Crimean-Congo Hemorrhagic Fever Virus
title_short Differential Cell Line Susceptibility to Crimean-Congo Hemorrhagic Fever Virus
title_sort differential cell line susceptibility to crimean-congo hemorrhagic fever virus
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8044861/
https://www.ncbi.nlm.nih.gov/pubmed/33869079
http://dx.doi.org/10.3389/fcimb.2021.648077
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