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Pre-Existing Humoral Immunity Enhances Epicutaneously-Administered Allergen Capture by Skin DC and Their Migration to Local Lymph Nodes

Due to its richness in antigen presenting cells, e.g., dendritic cells (DC), the skin has been identified as a promising route for immunotherapy and vaccination. Several years ago, a skin delivery system was developed based on epicutaneous patches allowing the administration of antigen through intac...

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Autores principales: Hervé, Pierre-Louis, Plaquet, Camille, Assoun, Noémie, Oreal, Nathalie, Gaulme, Laetitia, Perrin, Audrey, Bouzereau, Adeline, Dhelft, Véronique, Labernardière, Jean-Louis, Mondoulet, Lucie, Sampson, Hugh A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8044905/
https://www.ncbi.nlm.nih.gov/pubmed/33868229
http://dx.doi.org/10.3389/fimmu.2021.609029
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author Hervé, Pierre-Louis
Plaquet, Camille
Assoun, Noémie
Oreal, Nathalie
Gaulme, Laetitia
Perrin, Audrey
Bouzereau, Adeline
Dhelft, Véronique
Labernardière, Jean-Louis
Mondoulet, Lucie
Sampson, Hugh A.
author_facet Hervé, Pierre-Louis
Plaquet, Camille
Assoun, Noémie
Oreal, Nathalie
Gaulme, Laetitia
Perrin, Audrey
Bouzereau, Adeline
Dhelft, Véronique
Labernardière, Jean-Louis
Mondoulet, Lucie
Sampson, Hugh A.
author_sort Hervé, Pierre-Louis
collection PubMed
description Due to its richness in antigen presenting cells, e.g., dendritic cells (DC), the skin has been identified as a promising route for immunotherapy and vaccination. Several years ago, a skin delivery system was developed based on epicutaneous patches allowing the administration of antigen through intact skin. Using mouse models, we have shown that epicutaneous allergen application leads to a rapid uptake and transport of allergen-positive cells to skin-draining lymph nodes (LN). This occurred primarily in animals previously sensitized to the same allergen. In that context, we sought to better understand the role of the specific preexisting immunity in allergen capture by skin DC and their subsequent migration to LN. Specifically, we investigated the role of humoral immunity induced by sensitization and the involvement of IgG Fc receptors (FcγR). Epicutaneous patches containing fluorescently-labeled ovalbumin (OVA) were applied to naïve mice that had previously received either sera or purified IgG isolated from OVA-sensitized mice. To investigate the involvement of FcγR, animals received 2.4G2 (anti-FcγRII/RIII) blocking antibody, 24 hours before patch application. Mice that received sera or purified IgG originating from OVA-sensitized mice showed an increase in the quantity of OVA-positive DC in skin and LN. Moreover, the blockade of FcγR reduced the number of OVA-positive DC in LN to a level similar to that observed in naïve animals. Overall, these results demonstrate that preexisting specific-IgG antibodies are involved in allergen capture by skin DC following EPIT through the involvement of antigen-specific IgG-FcγR.
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spelling pubmed-80449052021-04-15 Pre-Existing Humoral Immunity Enhances Epicutaneously-Administered Allergen Capture by Skin DC and Their Migration to Local Lymph Nodes Hervé, Pierre-Louis Plaquet, Camille Assoun, Noémie Oreal, Nathalie Gaulme, Laetitia Perrin, Audrey Bouzereau, Adeline Dhelft, Véronique Labernardière, Jean-Louis Mondoulet, Lucie Sampson, Hugh A. Front Immunol Immunology Due to its richness in antigen presenting cells, e.g., dendritic cells (DC), the skin has been identified as a promising route for immunotherapy and vaccination. Several years ago, a skin delivery system was developed based on epicutaneous patches allowing the administration of antigen through intact skin. Using mouse models, we have shown that epicutaneous allergen application leads to a rapid uptake and transport of allergen-positive cells to skin-draining lymph nodes (LN). This occurred primarily in animals previously sensitized to the same allergen. In that context, we sought to better understand the role of the specific preexisting immunity in allergen capture by skin DC and their subsequent migration to LN. Specifically, we investigated the role of humoral immunity induced by sensitization and the involvement of IgG Fc receptors (FcγR). Epicutaneous patches containing fluorescently-labeled ovalbumin (OVA) were applied to naïve mice that had previously received either sera or purified IgG isolated from OVA-sensitized mice. To investigate the involvement of FcγR, animals received 2.4G2 (anti-FcγRII/RIII) blocking antibody, 24 hours before patch application. Mice that received sera or purified IgG originating from OVA-sensitized mice showed an increase in the quantity of OVA-positive DC in skin and LN. Moreover, the blockade of FcγR reduced the number of OVA-positive DC in LN to a level similar to that observed in naïve animals. Overall, these results demonstrate that preexisting specific-IgG antibodies are involved in allergen capture by skin DC following EPIT through the involvement of antigen-specific IgG-FcγR. Frontiers Media S.A. 2021-03-26 /pmc/articles/PMC8044905/ /pubmed/33868229 http://dx.doi.org/10.3389/fimmu.2021.609029 Text en Copyright © 2021 Hervé, Plaquet, Assoun, Oreal, Gaulme, Perrin, Bouzereau, Dhelft, Labernardière, Mondoulet and Sampson https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Hervé, Pierre-Louis
Plaquet, Camille
Assoun, Noémie
Oreal, Nathalie
Gaulme, Laetitia
Perrin, Audrey
Bouzereau, Adeline
Dhelft, Véronique
Labernardière, Jean-Louis
Mondoulet, Lucie
Sampson, Hugh A.
Pre-Existing Humoral Immunity Enhances Epicutaneously-Administered Allergen Capture by Skin DC and Their Migration to Local Lymph Nodes
title Pre-Existing Humoral Immunity Enhances Epicutaneously-Administered Allergen Capture by Skin DC and Their Migration to Local Lymph Nodes
title_full Pre-Existing Humoral Immunity Enhances Epicutaneously-Administered Allergen Capture by Skin DC and Their Migration to Local Lymph Nodes
title_fullStr Pre-Existing Humoral Immunity Enhances Epicutaneously-Administered Allergen Capture by Skin DC and Their Migration to Local Lymph Nodes
title_full_unstemmed Pre-Existing Humoral Immunity Enhances Epicutaneously-Administered Allergen Capture by Skin DC and Their Migration to Local Lymph Nodes
title_short Pre-Existing Humoral Immunity Enhances Epicutaneously-Administered Allergen Capture by Skin DC and Their Migration to Local Lymph Nodes
title_sort pre-existing humoral immunity enhances epicutaneously-administered allergen capture by skin dc and their migration to local lymph nodes
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8044905/
https://www.ncbi.nlm.nih.gov/pubmed/33868229
http://dx.doi.org/10.3389/fimmu.2021.609029
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