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Physicochemical Approach to Understanding the Structure, Conformation, and Activity of Mannan Polysaccharides

[Image: see text] Extracellular polysaccharides are widely produced by bacteria, yeasts, and algae. These polymers are involved in several biological functions, such as bacteria adhesion to surface and biofilm formation, ion sequestering, protection from desiccation, and cryoprotection. The chemical...

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Autores principales: Casillo, Angela, Fabozzi, Antonio, Russo Krauss, Irene, Parrilli, Ermenegilda, Biggs, Caroline I., Gibson, Matthew I., Lanzetta, Rosa, Appavou, Marie-Sousai, Radulescu, Aurel, Tutino, Maria L., Paduano, Luigi, Corsaro, Maria M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2021
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8045027/
https://www.ncbi.nlm.nih.gov/pubmed/33729771
http://dx.doi.org/10.1021/acs.biomac.0c01659
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author Casillo, Angela
Fabozzi, Antonio
Russo Krauss, Irene
Parrilli, Ermenegilda
Biggs, Caroline I.
Gibson, Matthew I.
Lanzetta, Rosa
Appavou, Marie-Sousai
Radulescu, Aurel
Tutino, Maria L.
Paduano, Luigi
Corsaro, Maria M.
author_facet Casillo, Angela
Fabozzi, Antonio
Russo Krauss, Irene
Parrilli, Ermenegilda
Biggs, Caroline I.
Gibson, Matthew I.
Lanzetta, Rosa
Appavou, Marie-Sousai
Radulescu, Aurel
Tutino, Maria L.
Paduano, Luigi
Corsaro, Maria M.
author_sort Casillo, Angela
collection PubMed
description [Image: see text] Extracellular polysaccharides are widely produced by bacteria, yeasts, and algae. These polymers are involved in several biological functions, such as bacteria adhesion to surface and biofilm formation, ion sequestering, protection from desiccation, and cryoprotection. The chemical characterization of these polymers is the starting point for obtaining relationships between their structures and their various functions. While this fundamental correlation is well reported and studied for the proteins, for the polysaccharides, this relationship is less intuitive. In this paper, we elucidate the chemical structure and conformational studies of a mannan exopolysaccharide from the permafrost isolated bacterium Psychrobacter arcticus strain 273-4. The mannan from the cold-adapted bacterium was compared with its dephosphorylated derivative and the commercial product from Saccharomyces cerevisiae. Starting from the chemical structure, we explored a new approach to deepen the study of the structure/activity relationship. A pool of physicochemical techniques, ranging from small-angle neutron scattering (SANS) and dynamic and static light scattering (DLS and SLS, respectively) to circular dichroism (CD) and cryo-transmission electron microscopy (cryo-TEM), have been used. Finally, the ice recrystallization inhibition activity of the polysaccharides was explored. The experimental evidence suggests that the mannan exopolysaccharide from P. arcticus bacterium has an efficient interaction with the water molecules, and it is structurally characterized by rigid-rod regions assuming a 14-helix-type conformation.
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spelling pubmed-80450272021-04-14 Physicochemical Approach to Understanding the Structure, Conformation, and Activity of Mannan Polysaccharides Casillo, Angela Fabozzi, Antonio Russo Krauss, Irene Parrilli, Ermenegilda Biggs, Caroline I. Gibson, Matthew I. Lanzetta, Rosa Appavou, Marie-Sousai Radulescu, Aurel Tutino, Maria L. Paduano, Luigi Corsaro, Maria M. Biomacromolecules [Image: see text] Extracellular polysaccharides are widely produced by bacteria, yeasts, and algae. These polymers are involved in several biological functions, such as bacteria adhesion to surface and biofilm formation, ion sequestering, protection from desiccation, and cryoprotection. The chemical characterization of these polymers is the starting point for obtaining relationships between their structures and their various functions. While this fundamental correlation is well reported and studied for the proteins, for the polysaccharides, this relationship is less intuitive. In this paper, we elucidate the chemical structure and conformational studies of a mannan exopolysaccharide from the permafrost isolated bacterium Psychrobacter arcticus strain 273-4. The mannan from the cold-adapted bacterium was compared with its dephosphorylated derivative and the commercial product from Saccharomyces cerevisiae. Starting from the chemical structure, we explored a new approach to deepen the study of the structure/activity relationship. A pool of physicochemical techniques, ranging from small-angle neutron scattering (SANS) and dynamic and static light scattering (DLS and SLS, respectively) to circular dichroism (CD) and cryo-transmission electron microscopy (cryo-TEM), have been used. Finally, the ice recrystallization inhibition activity of the polysaccharides was explored. The experimental evidence suggests that the mannan exopolysaccharide from P. arcticus bacterium has an efficient interaction with the water molecules, and it is structurally characterized by rigid-rod regions assuming a 14-helix-type conformation. American Chemical Society 2021-03-17 2021-04-12 /pmc/articles/PMC8045027/ /pubmed/33729771 http://dx.doi.org/10.1021/acs.biomac.0c01659 Text en © 2021 The Authors. Published by American Chemical Society Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Casillo, Angela
Fabozzi, Antonio
Russo Krauss, Irene
Parrilli, Ermenegilda
Biggs, Caroline I.
Gibson, Matthew I.
Lanzetta, Rosa
Appavou, Marie-Sousai
Radulescu, Aurel
Tutino, Maria L.
Paduano, Luigi
Corsaro, Maria M.
Physicochemical Approach to Understanding the Structure, Conformation, and Activity of Mannan Polysaccharides
title Physicochemical Approach to Understanding the Structure, Conformation, and Activity of Mannan Polysaccharides
title_full Physicochemical Approach to Understanding the Structure, Conformation, and Activity of Mannan Polysaccharides
title_fullStr Physicochemical Approach to Understanding the Structure, Conformation, and Activity of Mannan Polysaccharides
title_full_unstemmed Physicochemical Approach to Understanding the Structure, Conformation, and Activity of Mannan Polysaccharides
title_short Physicochemical Approach to Understanding the Structure, Conformation, and Activity of Mannan Polysaccharides
title_sort physicochemical approach to understanding the structure, conformation, and activity of mannan polysaccharides
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8045027/
https://www.ncbi.nlm.nih.gov/pubmed/33729771
http://dx.doi.org/10.1021/acs.biomac.0c01659
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