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mRNA-Seq reveals the quorum sensing system luxS gene contributes to the environmental fitness of Streptococcus suis type 2
BACKGROUND: Streptococcus suis type 2 (SS2) is an important zoonotic pathogen. We have previously reported the structure of LuxS protein and found that the luxS gene is closely related to biofilm, virulence gene expression and drug resistance of SS2. However, the mechanism of luxS mediated SS2 stres...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8045309/ https://www.ncbi.nlm.nih.gov/pubmed/33849451 http://dx.doi.org/10.1186/s12866-021-02170-w |
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author | Li, Jinpeng Wang, Yuxin Du, Yanbin Zhang, Hui Fan, Qingying Sun, Liyun Yi, Li Wang, Shaohui Wang, Yang |
author_facet | Li, Jinpeng Wang, Yuxin Du, Yanbin Zhang, Hui Fan, Qingying Sun, Liyun Yi, Li Wang, Shaohui Wang, Yang |
author_sort | Li, Jinpeng |
collection | PubMed |
description | BACKGROUND: Streptococcus suis type 2 (SS2) is an important zoonotic pathogen. We have previously reported the structure of LuxS protein and found that the luxS gene is closely related to biofilm, virulence gene expression and drug resistance of SS2. However, the mechanism of luxS mediated SS2 stress response is unclear. Therefore, this experiment performed stress response to luxS mutant (ΔluxS) and complement strain (CΔluxS), overexpression strain (luxS+) and wild-type SS2 strain HA9801, and analyzed the differential phenotypes in combination with transcriptome data. RESULTS: The results indicate that the luxS gene deletion causes a wide range of phenotypic changes, including chain length. RNA sequencing identified 278 lx-regulated genes, of which 179 were up-regulated and 99 were down-regulated. Differential genes focus on bacterial growth, stress response, metabolic mechanisms and drug tolerance. Multiple mitotic genes were down-regulated; while the ABC transporter system genes, cobalamin /Fe(3+)-iron carrier ABC transporter ATPase and oxidative stress regulators were up-regulated. The inactivation of the luxS gene caused a significant reduction in the growth and survival in the acid (pH = 3.0, 4.0, 5.0) and iron (100 mM iron chelator 2,2′-dipyridyl) stress environments. However, the mutant strain ΔluxS showed increased antioxidant activity to H(2)O(2) (58.8 mmol/L). CONCLUSIONS: The luxS gene in SS2 appears to play roles in iron metabolism and protective responses to acidic and oxidative environmental conditions. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12866-021-02170-w. |
format | Online Article Text |
id | pubmed-8045309 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-80453092021-04-14 mRNA-Seq reveals the quorum sensing system luxS gene contributes to the environmental fitness of Streptococcus suis type 2 Li, Jinpeng Wang, Yuxin Du, Yanbin Zhang, Hui Fan, Qingying Sun, Liyun Yi, Li Wang, Shaohui Wang, Yang BMC Microbiol Research Article BACKGROUND: Streptococcus suis type 2 (SS2) is an important zoonotic pathogen. We have previously reported the structure of LuxS protein and found that the luxS gene is closely related to biofilm, virulence gene expression and drug resistance of SS2. However, the mechanism of luxS mediated SS2 stress response is unclear. Therefore, this experiment performed stress response to luxS mutant (ΔluxS) and complement strain (CΔluxS), overexpression strain (luxS+) and wild-type SS2 strain HA9801, and analyzed the differential phenotypes in combination with transcriptome data. RESULTS: The results indicate that the luxS gene deletion causes a wide range of phenotypic changes, including chain length. RNA sequencing identified 278 lx-regulated genes, of which 179 were up-regulated and 99 were down-regulated. Differential genes focus on bacterial growth, stress response, metabolic mechanisms and drug tolerance. Multiple mitotic genes were down-regulated; while the ABC transporter system genes, cobalamin /Fe(3+)-iron carrier ABC transporter ATPase and oxidative stress regulators were up-regulated. The inactivation of the luxS gene caused a significant reduction in the growth and survival in the acid (pH = 3.0, 4.0, 5.0) and iron (100 mM iron chelator 2,2′-dipyridyl) stress environments. However, the mutant strain ΔluxS showed increased antioxidant activity to H(2)O(2) (58.8 mmol/L). CONCLUSIONS: The luxS gene in SS2 appears to play roles in iron metabolism and protective responses to acidic and oxidative environmental conditions. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12866-021-02170-w. BioMed Central 2021-04-13 /pmc/articles/PMC8045309/ /pubmed/33849451 http://dx.doi.org/10.1186/s12866-021-02170-w Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Article Li, Jinpeng Wang, Yuxin Du, Yanbin Zhang, Hui Fan, Qingying Sun, Liyun Yi, Li Wang, Shaohui Wang, Yang mRNA-Seq reveals the quorum sensing system luxS gene contributes to the environmental fitness of Streptococcus suis type 2 |
title | mRNA-Seq reveals the quorum sensing system luxS gene contributes to the environmental fitness of Streptococcus suis type 2 |
title_full | mRNA-Seq reveals the quorum sensing system luxS gene contributes to the environmental fitness of Streptococcus suis type 2 |
title_fullStr | mRNA-Seq reveals the quorum sensing system luxS gene contributes to the environmental fitness of Streptococcus suis type 2 |
title_full_unstemmed | mRNA-Seq reveals the quorum sensing system luxS gene contributes to the environmental fitness of Streptococcus suis type 2 |
title_short | mRNA-Seq reveals the quorum sensing system luxS gene contributes to the environmental fitness of Streptococcus suis type 2 |
title_sort | mrna-seq reveals the quorum sensing system luxs gene contributes to the environmental fitness of streptococcus suis type 2 |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8045309/ https://www.ncbi.nlm.nih.gov/pubmed/33849451 http://dx.doi.org/10.1186/s12866-021-02170-w |
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