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General Control Non-derepressible 1 (AtGCN1) Is Important for Flowering Time, Plant Growth, Seed Development, and the Transcription/Translation of Specific Genes in Arabidopsis

We have previously demonstrated that General Control Non-derepressible 1 (AtGCN1) is essential for translation inhibition under cold stress through interacting with GCN2 to phosphorylate eukaryotic translation initiation factor 2 (eIF2). Here, we report that the flower time of the atgcn1 mutant is l...

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Autores principales: Cui, Xiaona, Gao, Kaili, Wang, Linjuan, Lv, Mengyang, Li, Ziwen, Zheng, Donghua, Wu, Wenwu, Yao, Wen, Ding, Liying, Li, Xiao, Zhu, Jian-Kang, Zhang, Hairong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8045761/
https://www.ncbi.nlm.nih.gov/pubmed/33868334
http://dx.doi.org/10.3389/fpls.2021.630311
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author Cui, Xiaona
Gao, Kaili
Wang, Linjuan
Lv, Mengyang
Li, Ziwen
Zheng, Donghua
Wu, Wenwu
Yao, Wen
Ding, Liying
Li, Xiao
Zhu, Jian-Kang
Zhang, Hairong
author_facet Cui, Xiaona
Gao, Kaili
Wang, Linjuan
Lv, Mengyang
Li, Ziwen
Zheng, Donghua
Wu, Wenwu
Yao, Wen
Ding, Liying
Li, Xiao
Zhu, Jian-Kang
Zhang, Hairong
author_sort Cui, Xiaona
collection PubMed
description We have previously demonstrated that General Control Non-derepressible 1 (AtGCN1) is essential for translation inhibition under cold stress through interacting with GCN2 to phosphorylate eukaryotic translation initiation factor 2 (eIF2). Here, we report that the flower time of the atgcn1 mutant is later than that of the wild type (WT), and some siliques of atgcn1 cannot develop and produce seeds. Total and polysomal RNA of atgcn1-1 and wild type (WT) after cold treatments were sequenced. The sequencing results show that the mutation of atgcn1 selectively alters the expression of genes at both transcriptional and translational levels. The classification of AtGCN1 target genes reveals that AtGCN1 regulated gens are involved in flower development, seed dormancy and seed development, response to osmotic stress, amino acid biosynthesis, photosynthesis, cell wall organization, protein transport and localization, lipid biosynthesis, transcription, macroautophagy, proteolysis and cell death. Further analysis of AtGCN1 regulated genes at translational levels shows that the Kozak sequence and uORFs (upstream open reading frame) of transcripts affect translation selection. These results show that AtGCN1 is required for the expression of selective genes in Arabidopsis.
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spelling pubmed-80457612021-04-15 General Control Non-derepressible 1 (AtGCN1) Is Important for Flowering Time, Plant Growth, Seed Development, and the Transcription/Translation of Specific Genes in Arabidopsis Cui, Xiaona Gao, Kaili Wang, Linjuan Lv, Mengyang Li, Ziwen Zheng, Donghua Wu, Wenwu Yao, Wen Ding, Liying Li, Xiao Zhu, Jian-Kang Zhang, Hairong Front Plant Sci Plant Science We have previously demonstrated that General Control Non-derepressible 1 (AtGCN1) is essential for translation inhibition under cold stress through interacting with GCN2 to phosphorylate eukaryotic translation initiation factor 2 (eIF2). Here, we report that the flower time of the atgcn1 mutant is later than that of the wild type (WT), and some siliques of atgcn1 cannot develop and produce seeds. Total and polysomal RNA of atgcn1-1 and wild type (WT) after cold treatments were sequenced. The sequencing results show that the mutation of atgcn1 selectively alters the expression of genes at both transcriptional and translational levels. The classification of AtGCN1 target genes reveals that AtGCN1 regulated gens are involved in flower development, seed dormancy and seed development, response to osmotic stress, amino acid biosynthesis, photosynthesis, cell wall organization, protein transport and localization, lipid biosynthesis, transcription, macroautophagy, proteolysis and cell death. Further analysis of AtGCN1 regulated genes at translational levels shows that the Kozak sequence and uORFs (upstream open reading frame) of transcripts affect translation selection. These results show that AtGCN1 is required for the expression of selective genes in Arabidopsis. Frontiers Media S.A. 2021-03-31 /pmc/articles/PMC8045761/ /pubmed/33868334 http://dx.doi.org/10.3389/fpls.2021.630311 Text en Copyright © 2021 Cui, Gao, Wang, Lv, Li, Zheng, Wu, Yao, Ding, Li, Zhu and Zhang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Cui, Xiaona
Gao, Kaili
Wang, Linjuan
Lv, Mengyang
Li, Ziwen
Zheng, Donghua
Wu, Wenwu
Yao, Wen
Ding, Liying
Li, Xiao
Zhu, Jian-Kang
Zhang, Hairong
General Control Non-derepressible 1 (AtGCN1) Is Important for Flowering Time, Plant Growth, Seed Development, and the Transcription/Translation of Specific Genes in Arabidopsis
title General Control Non-derepressible 1 (AtGCN1) Is Important for Flowering Time, Plant Growth, Seed Development, and the Transcription/Translation of Specific Genes in Arabidopsis
title_full General Control Non-derepressible 1 (AtGCN1) Is Important for Flowering Time, Plant Growth, Seed Development, and the Transcription/Translation of Specific Genes in Arabidopsis
title_fullStr General Control Non-derepressible 1 (AtGCN1) Is Important for Flowering Time, Plant Growth, Seed Development, and the Transcription/Translation of Specific Genes in Arabidopsis
title_full_unstemmed General Control Non-derepressible 1 (AtGCN1) Is Important for Flowering Time, Plant Growth, Seed Development, and the Transcription/Translation of Specific Genes in Arabidopsis
title_short General Control Non-derepressible 1 (AtGCN1) Is Important for Flowering Time, Plant Growth, Seed Development, and the Transcription/Translation of Specific Genes in Arabidopsis
title_sort general control non-derepressible 1 (atgcn1) is important for flowering time, plant growth, seed development, and the transcription/translation of specific genes in arabidopsis
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8045761/
https://www.ncbi.nlm.nih.gov/pubmed/33868334
http://dx.doi.org/10.3389/fpls.2021.630311
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