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Cotransplantation of mesenchymal stem cells and endothelial progenitor cells for treating steroid‐induced osteonecrosis of the femoral head
Steroid‐induced osteonecrosis of the femoral head (ONFH) is characterized by decreased osteogenesis, angiogenesis, and increased adipogenesis. While bone tissue engineering has been widely investigated to treat ONFH, its therapeutic effects remain unsatisfactory. Therefore, further studies are requi...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley & Sons, Inc.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8046137/ https://www.ncbi.nlm.nih.gov/pubmed/33438370 http://dx.doi.org/10.1002/sctm.20-0346 |
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author | Xu, Haixia Wang, Chengqiang Liu, Chun Peng, Ziyue Li, Jianjun Jin, Yanglei Wang, Yihan Guo, Jiasong Zhu, Lixin |
author_facet | Xu, Haixia Wang, Chengqiang Liu, Chun Peng, Ziyue Li, Jianjun Jin, Yanglei Wang, Yihan Guo, Jiasong Zhu, Lixin |
author_sort | Xu, Haixia |
collection | PubMed |
description | Steroid‐induced osteonecrosis of the femoral head (ONFH) is characterized by decreased osteogenesis, angiogenesis, and increased adipogenesis. While bone tissue engineering has been widely investigated to treat ONFH, its therapeutic effects remain unsatisfactory. Therefore, further studies are required to determine optimal osteogenesis, angiogenesis and adipogenesis in the necrotic area of the femoral head. In our study, we developed a carboxymethyl chitosan/alginate/bone marrow mesenchymal stem cell/endothelial progenitor cell (CMC/ALG/BMSC/EPC) composite implant, and evaluated its ability to repair steroid‐induced ONFH. Our in vitro studies showed that BMSC and EPC coculture displayed enhanced osteogenic and angiogenic differentiation. When compared with single BMSC cultures, adipogenic differentiation in coculture systems was reduced. We also fabricated a three‐dimensional (3D) CMC/ALG scaffold for loading cells, using a lyophilization approach, and confirmed its good cell compatibility characteristics, that is, high porosity, low cytotoxicity and favorable cell adhesion. 3D coculture of BMSCs and EPCs also promoted secretion of osteogenic and angiogenic factors. Then, we established an rabbit model of steroid‐induced ONFH. The CMC/ALG/BMSC/EPC composite implant was transplanted into the bone tunnel of the rabbit femoral head after core decompression (CD) surgery. Twelve weeks later, radiographical and histological analyses revealed CMC/ALG/BMSC/EPC composite implants had facilitated the repair of steroid‐induced ONFH, by promoting osteogenesis and angiogenesis, and reducing adipogenesis when compared with CD, CMC/ALG, CMC/ALG/BMSC and CMC/ALG/EPC groups. Thus, our data show that cotransplantation of BMSCs and EPCs in 3D scaffolds is beneficial in treating steroid‐induced ONFH. |
format | Online Article Text |
id | pubmed-8046137 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | John Wiley & Sons, Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-80461372021-04-16 Cotransplantation of mesenchymal stem cells and endothelial progenitor cells for treating steroid‐induced osteonecrosis of the femoral head Xu, Haixia Wang, Chengqiang Liu, Chun Peng, Ziyue Li, Jianjun Jin, Yanglei Wang, Yihan Guo, Jiasong Zhu, Lixin Stem Cells Transl Med Tissue Engineering and Regenerative Medicine Steroid‐induced osteonecrosis of the femoral head (ONFH) is characterized by decreased osteogenesis, angiogenesis, and increased adipogenesis. While bone tissue engineering has been widely investigated to treat ONFH, its therapeutic effects remain unsatisfactory. Therefore, further studies are required to determine optimal osteogenesis, angiogenesis and adipogenesis in the necrotic area of the femoral head. In our study, we developed a carboxymethyl chitosan/alginate/bone marrow mesenchymal stem cell/endothelial progenitor cell (CMC/ALG/BMSC/EPC) composite implant, and evaluated its ability to repair steroid‐induced ONFH. Our in vitro studies showed that BMSC and EPC coculture displayed enhanced osteogenic and angiogenic differentiation. When compared with single BMSC cultures, adipogenic differentiation in coculture systems was reduced. We also fabricated a three‐dimensional (3D) CMC/ALG scaffold for loading cells, using a lyophilization approach, and confirmed its good cell compatibility characteristics, that is, high porosity, low cytotoxicity and favorable cell adhesion. 3D coculture of BMSCs and EPCs also promoted secretion of osteogenic and angiogenic factors. Then, we established an rabbit model of steroid‐induced ONFH. The CMC/ALG/BMSC/EPC composite implant was transplanted into the bone tunnel of the rabbit femoral head after core decompression (CD) surgery. Twelve weeks later, radiographical and histological analyses revealed CMC/ALG/BMSC/EPC composite implants had facilitated the repair of steroid‐induced ONFH, by promoting osteogenesis and angiogenesis, and reducing adipogenesis when compared with CD, CMC/ALG, CMC/ALG/BMSC and CMC/ALG/EPC groups. Thus, our data show that cotransplantation of BMSCs and EPCs in 3D scaffolds is beneficial in treating steroid‐induced ONFH. John Wiley & Sons, Inc. 2021-01-13 /pmc/articles/PMC8046137/ /pubmed/33438370 http://dx.doi.org/10.1002/sctm.20-0346 Text en © 2021 The Authors. stem cells translational medicine published by Wiley Periodicals LLC on behalf of AlphaMed Press https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Tissue Engineering and Regenerative Medicine Xu, Haixia Wang, Chengqiang Liu, Chun Peng, Ziyue Li, Jianjun Jin, Yanglei Wang, Yihan Guo, Jiasong Zhu, Lixin Cotransplantation of mesenchymal stem cells and endothelial progenitor cells for treating steroid‐induced osteonecrosis of the femoral head |
title | Cotransplantation of mesenchymal stem cells and endothelial progenitor cells for treating steroid‐induced osteonecrosis of the femoral head |
title_full | Cotransplantation of mesenchymal stem cells and endothelial progenitor cells for treating steroid‐induced osteonecrosis of the femoral head |
title_fullStr | Cotransplantation of mesenchymal stem cells and endothelial progenitor cells for treating steroid‐induced osteonecrosis of the femoral head |
title_full_unstemmed | Cotransplantation of mesenchymal stem cells and endothelial progenitor cells for treating steroid‐induced osteonecrosis of the femoral head |
title_short | Cotransplantation of mesenchymal stem cells and endothelial progenitor cells for treating steroid‐induced osteonecrosis of the femoral head |
title_sort | cotransplantation of mesenchymal stem cells and endothelial progenitor cells for treating steroid‐induced osteonecrosis of the femoral head |
topic | Tissue Engineering and Regenerative Medicine |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8046137/ https://www.ncbi.nlm.nih.gov/pubmed/33438370 http://dx.doi.org/10.1002/sctm.20-0346 |
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