Cargando…

Cryopreservation of human cerebral microvascular endothelial cells and astrocytes in suspension and monolayers

The blood–brain barrier (BBB) keeps pathogens and toxins out of the brain but also impedes the entry of pharmaceuticals. Human cerebral microvascular endothelial cells (hCMECs) and astrocytes are the main functional cell components of the BBB. Although available commercially as cryopreserved cells i...

Descripción completa

Detalles Bibliográficos
Autores principales:	Marquez-Curtis, Leah A., Bokenfohr, Reid, McGann, Locksley E., Elliott, Janet A. W.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2021
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8046249/ https://www.ncbi.nlm.nih.gov/pubmed/33852594 http://dx.doi.org/10.1371/journal.pone.0249814

Ejemplares similares

Improved Cryopreservation of Human Umbilical Vein Endothelial Cells: A Systematic Approach
por: Sultani, A. Billal, et al.
Publicado: (2016)

Cryopreservation and post-thaw characterization of dissociated human islet cells
por: Marquez-Curtis, Leah A., et al.
Publicado: (2022)

The Influence of Capsaicin on the Integrity of Microvascular Endothelial Cell Monolayers
por: Kaiser, Mathias, et al.
Publicado: (2018)

Serum- and albumin-free cryopreservation of endothelial monolayers with a new solution
por: Pless-Petig, Gesine, et al.
Publicado: (2018)

In vitro model of the blood-brain barrier established by co-culture of primary cerebral microvascular endothelial and astrocyte cells
por: Wang, Yan, et al.
Publicado: (2015)

Differential Cellular Effects of Electroporation and Electrochemotherapy in Monolayers of Human Microvascular Endothelial Cells
por: Meulenberg, Cécil J. W., et al.
Publicado: (2012)

Extracellular Antifreeze Protein Significantly Enhances the Cryopreservation of Cell Monolayers
por: Tomás, Ruben M. F., et al.
Publicado: (2019)

Assay-ready Cryopreserved Cell Monolayers Enabled by Macromolecular Cryoprotectants
por: Tomás, Ruben M. F., et al.
Publicado: (2022)

Microvascular endothelial cells-derived microvesicles imply in ischemic stroke by modulating astrocyte and blood brain barrier function and cerebral blood flow
por: Pan, Qunwen, et al.
Publicado: (2016)

Multifunctional Liposomes Modulate Purinergic Receptor-Induced Calcium Wave in Cerebral Microvascular Endothelial Cells and Astrocytes: New Insights for Alzheimer’s disease
por: Forcaia, Greta, et al.
Publicado: (2021)

Endothelial depletion of Atg7 triggers astrocyte–microvascular disassociation at blood–brain barrier
por: Liu, Hui, et al.
Publicado: (2023)

Astrocyte-derived VEGF increases cerebral microvascular permeability under high salt conditions
por: Deng, Zhezhi, et al.
Publicado: (2020)

Exposure to Blast Overpressure Impairs Cerebral Microvascular Responses and Alters Vascular and Astrocytic Structure
por: Abutarboush, Rania, et al.
Publicado: (2019)

Validation and reproducibility of computerised cell-viability analysis of tissue slices
por: Jomha, NM, et al.
Publicado: (2003)

Washing out the Uterine Cavity
por: McGann,
Publicado: (1883)

A Novel Brain Neurovascular Unit Model with Neurons, Astrocytes and Microvascular Endothelial Cells of Rat
por: Xue, Qiang, et al.
Publicado: (2013)

The multifaceted responses of primary human astrocytes and brain microvascular endothelial cells to the Lyme disease spirochete, Borrelia burgdorferi
por: Brissette, Catherine A., et al.
Publicado: (2013)

Polyproline as a Minimal Antifreeze Protein Mimic That Enhances the Cryopreservation of Cell Monolayers
por: Graham, Ben, et al.
Publicado: (2017)

Enacting a social ecology: radically embodied intersubjectivity
por: McGann, Marek
Publicado: (2014)

A Case of Traumatic Tetanus; Recovery
por: McGann, T. J.
Publicado: (1883)

Amputation of the Penis
por: McGann, T. J.
Publicado: (1883)

Internal Urethrotomy
por: McGann, T. J.
Publicado: (1883)

Case of Eversion of Bladder
por: McGann, T. J.
Publicado: (1879)

Remarks on a Case of Amputation of the Penis
por: McGann, T. J.
Publicado: (1878)

Lead as an Application to Wounds; Its Antiphlogistic and Antiseptic Properties
por: McGann, T. J.
Publicado: (1876)

Stricture at External Meatus, with Phimosis, Followed by Abscess and Fistula; Remarks
por: McGann, T. J.
Publicado: (1876)

Case of Human Sacrifice, Suicidal or Murderous
por: McGann, T. J.
Publicado: (1875)

Convergently Emergent: Ecological and Enactive Approaches to the Texture of Agency
por: McGann, Marek
Publicado: (2020)

Glutathione in Cerebral Microvascular Endothelial Biology and Pathobiology: Implications for Brain Homeostasis
por: Li, Wei, et al.
Publicado: (2012)

Astrocytes Protect Human Brain Microvascular Endothelial Cells from Hypoxia Injury by Regulating VEGF Expression
por: Liu, Jianmin, et al.
Publicado: (2022)

IGF2 and IGF1R identified as novel tip cell genes in primary microvascular endothelial cell monolayers
por: Dallinga, Marchien G., et al.
Publicado: (2018)

Influenza H7N9 virus disrupts the monolayer human brain microvascular endothelial cells barrier in vitro
por: Lei, Yuxuan, et al.
Publicado: (2023)

Cryopreservation of testicular tissue or testicular cell suspensions: a pivotal step in fertility preservation
por: Onofre, J., et al.
Publicado: (2016)

Associative learning and sensory neuroplasticity: how does it happen and what is it good for?
por: McGann, John P.
Publicado: (2015)

Respiratory Tract Symptom Complexes
por: McGann, Kathleen A., et al.
Publicado: (2018)

Septins regulate junctional integrity of endothelial monolayers
por: Kim, Joanna, et al.
Publicado: (2018)

Cryopreservation and Enumeration of Human Endothelial Progenitor and Endothelial Cells for Clinical Trials
por: Bogoslovsky, T, et al.
Publicado: (2013)

Functional brain-specific microvessels from iPSC-derived human brain microvascular endothelial cells: the role of matrix composition on monolayer formation
por: Katt, Moriah E., et al.
Publicado: (2018)

How Mean is the Mean?
por: Speelman, Craig P., et al.
Publicado: (2013)

Sampling Participants’ Experience in Laboratory Experiments: Complementary Challenges for More Complete Data Collection
por: McAuliffe, Alan, et al.
Publicado: (2016)

Cannot write session to /tmp/vufind_sessions/sess_3ig6sjglnkbd1vq8lim4sdclm1