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Development of a novel heterologous gene expression system using earthworms
In recent years, animals and plants have received increasing attention as potential next-generation protein production systems, especially for biopharmaceuticals and animal proteins. The aim of the present study was to develop the earthworms Eisenia fetida Waki and Eisenia andrei Sagami as next-gene...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8046771/ https://www.ncbi.nlm.nih.gov/pubmed/33854163 http://dx.doi.org/10.1038/s41598-021-87641-w |
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author | Akazawa, Shin-ichi Machida, Yu Takeuchi, Aya Wakatsuki, Yuka Kanda, Naoki Kashima, Norito Murayama, Hayato |
author_facet | Akazawa, Shin-ichi Machida, Yu Takeuchi, Aya Wakatsuki, Yuka Kanda, Naoki Kashima, Norito Murayama, Hayato |
author_sort | Akazawa, Shin-ichi |
collection | PubMed |
description | In recent years, animals and plants have received increasing attention as potential next-generation protein production systems, especially for biopharmaceuticals and animal proteins. The aim of the present study was to develop the earthworms Eisenia fetida Waki and Eisenia andrei Sagami as next-generation animal protein production hosts. These earthworms have been approved as model animals for acute toxicity tests by the Organization for Economic Co-operation and Development, and they have post-translational modification systems. However, so far, none of the studies have used earthworm transfection techniques. Thus, we developed a transfection method for E. fetida and E. andrei using microinjection and electroporation systems. The maximum survival rates and transfection efficiencies were 79.2% and 29.2% for E. fetida, and 95.8% and 50.0% for E. andrei, respectively. Furthermore, human erythropoietin was detected in the transformed earthworm tail fragments using an enzyme-linked immunosorbent assay. These results contribute to the development of a potential earthworm-based novel animal protein production system. |
format | Online Article Text |
id | pubmed-8046771 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-80467712021-04-15 Development of a novel heterologous gene expression system using earthworms Akazawa, Shin-ichi Machida, Yu Takeuchi, Aya Wakatsuki, Yuka Kanda, Naoki Kashima, Norito Murayama, Hayato Sci Rep Article In recent years, animals and plants have received increasing attention as potential next-generation protein production systems, especially for biopharmaceuticals and animal proteins. The aim of the present study was to develop the earthworms Eisenia fetida Waki and Eisenia andrei Sagami as next-generation animal protein production hosts. These earthworms have been approved as model animals for acute toxicity tests by the Organization for Economic Co-operation and Development, and they have post-translational modification systems. However, so far, none of the studies have used earthworm transfection techniques. Thus, we developed a transfection method for E. fetida and E. andrei using microinjection and electroporation systems. The maximum survival rates and transfection efficiencies were 79.2% and 29.2% for E. fetida, and 95.8% and 50.0% for E. andrei, respectively. Furthermore, human erythropoietin was detected in the transformed earthworm tail fragments using an enzyme-linked immunosorbent assay. These results contribute to the development of a potential earthworm-based novel animal protein production system. Nature Publishing Group UK 2021-04-14 /pmc/articles/PMC8046771/ /pubmed/33854163 http://dx.doi.org/10.1038/s41598-021-87641-w Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Akazawa, Shin-ichi Machida, Yu Takeuchi, Aya Wakatsuki, Yuka Kanda, Naoki Kashima, Norito Murayama, Hayato Development of a novel heterologous gene expression system using earthworms |
title | Development of a novel heterologous gene expression system using earthworms |
title_full | Development of a novel heterologous gene expression system using earthworms |
title_fullStr | Development of a novel heterologous gene expression system using earthworms |
title_full_unstemmed | Development of a novel heterologous gene expression system using earthworms |
title_short | Development of a novel heterologous gene expression system using earthworms |
title_sort | development of a novel heterologous gene expression system using earthworms |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8046771/ https://www.ncbi.nlm.nih.gov/pubmed/33854163 http://dx.doi.org/10.1038/s41598-021-87641-w |
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