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Unbiased proteomic profiling of host cell extracellular vesicle composition and dynamics upon HIV‐1 infection

Cells release diverse types of extracellular vesicles (EVs), which transfer complex signals to surrounding cells. Specific markers to distinguish different EVs (e.g. exosomes, ectosomes, enveloped viruses like HIV) are still lacking. We have developed a proteomic profiling approach for characterizin...

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Autores principales: Martin‐Jaular, Lorena, Nevo, Nathalie, Schessner, Julia P, Tkach, Mercedes, Jouve, Mabel, Dingli, Florent, Loew, Damarys, Witwer, Kenneth W, Ostrowski, Matias, Borner, Georg H H, Théry, Clotilde
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8047442/
https://www.ncbi.nlm.nih.gov/pubmed/33709510
http://dx.doi.org/10.15252/embj.2020105492
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author Martin‐Jaular, Lorena
Nevo, Nathalie
Schessner, Julia P
Tkach, Mercedes
Jouve, Mabel
Dingli, Florent
Loew, Damarys
Witwer, Kenneth W
Ostrowski, Matias
Borner, Georg H H
Théry, Clotilde
author_facet Martin‐Jaular, Lorena
Nevo, Nathalie
Schessner, Julia P
Tkach, Mercedes
Jouve, Mabel
Dingli, Florent
Loew, Damarys
Witwer, Kenneth W
Ostrowski, Matias
Borner, Georg H H
Théry, Clotilde
author_sort Martin‐Jaular, Lorena
collection PubMed
description Cells release diverse types of extracellular vesicles (EVs), which transfer complex signals to surrounding cells. Specific markers to distinguish different EVs (e.g. exosomes, ectosomes, enveloped viruses like HIV) are still lacking. We have developed a proteomic profiling approach for characterizing EV subtype composition and applied it to human Jurkat T cells. We generated an interactive database to define groups of proteins with similar profiles, suggesting release in similar EVs. Biochemical validation confirmed the presence of preferred partners of commonly used exosome markers in EVs: CD81/ADAM10/ITGB1, and CD63/syntenin. We then compared EVs from control and HIV‐1‐infected cells. HIV infection altered EV profiles of several cellular proteins, including MOV10 and SPN, which became incorporated into HIV virions, and SERINC3, which was re‐routed to non‐viral EVs in a Nef‐dependent manner. Furthermore, we found that SERINC3 controls the surface composition of EVs. Our workflow provides an unbiased approach for identifying candidate markers and potential regulators of EV subtypes. It can be widely applied to in vitro experimental systems for investigating physiological or pathological modifications of EV release.
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spelling pubmed-80474422021-04-16 Unbiased proteomic profiling of host cell extracellular vesicle composition and dynamics upon HIV‐1 infection Martin‐Jaular, Lorena Nevo, Nathalie Schessner, Julia P Tkach, Mercedes Jouve, Mabel Dingli, Florent Loew, Damarys Witwer, Kenneth W Ostrowski, Matias Borner, Georg H H Théry, Clotilde EMBO J Articles Cells release diverse types of extracellular vesicles (EVs), which transfer complex signals to surrounding cells. Specific markers to distinguish different EVs (e.g. exosomes, ectosomes, enveloped viruses like HIV) are still lacking. We have developed a proteomic profiling approach for characterizing EV subtype composition and applied it to human Jurkat T cells. We generated an interactive database to define groups of proteins with similar profiles, suggesting release in similar EVs. Biochemical validation confirmed the presence of preferred partners of commonly used exosome markers in EVs: CD81/ADAM10/ITGB1, and CD63/syntenin. We then compared EVs from control and HIV‐1‐infected cells. HIV infection altered EV profiles of several cellular proteins, including MOV10 and SPN, which became incorporated into HIV virions, and SERINC3, which was re‐routed to non‐viral EVs in a Nef‐dependent manner. Furthermore, we found that SERINC3 controls the surface composition of EVs. Our workflow provides an unbiased approach for identifying candidate markers and potential regulators of EV subtypes. It can be widely applied to in vitro experimental systems for investigating physiological or pathological modifications of EV release. John Wiley and Sons Inc. 2021-03-11 2021-04-15 /pmc/articles/PMC8047442/ /pubmed/33709510 http://dx.doi.org/10.15252/embj.2020105492 Text en © 2021 The Authors. Published under the terms of the CC BY NC ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Articles
Martin‐Jaular, Lorena
Nevo, Nathalie
Schessner, Julia P
Tkach, Mercedes
Jouve, Mabel
Dingli, Florent
Loew, Damarys
Witwer, Kenneth W
Ostrowski, Matias
Borner, Georg H H
Théry, Clotilde
Unbiased proteomic profiling of host cell extracellular vesicle composition and dynamics upon HIV‐1 infection
title Unbiased proteomic profiling of host cell extracellular vesicle composition and dynamics upon HIV‐1 infection
title_full Unbiased proteomic profiling of host cell extracellular vesicle composition and dynamics upon HIV‐1 infection
title_fullStr Unbiased proteomic profiling of host cell extracellular vesicle composition and dynamics upon HIV‐1 infection
title_full_unstemmed Unbiased proteomic profiling of host cell extracellular vesicle composition and dynamics upon HIV‐1 infection
title_short Unbiased proteomic profiling of host cell extracellular vesicle composition and dynamics upon HIV‐1 infection
title_sort unbiased proteomic profiling of host cell extracellular vesicle composition and dynamics upon hiv‐1 infection
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8047442/
https://www.ncbi.nlm.nih.gov/pubmed/33709510
http://dx.doi.org/10.15252/embj.2020105492
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