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Dendrobium nobile Lindl. alkaloids alleviate Mn-induced neurotoxicity via PINK1/Parkin-mediated mitophagy in PC12 cells

Modern pharmacological studies have demonstrated that Dendrobium nobile Lindl. Alkaloids (DNLA), the main active ingredients of Dendrobium nobile, is valuable as an anti-aging and neuroprotective herbal medicine. The present study was designed to determine whether DNLA confers protective function ov...

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Autores principales: Fu, Xiaolong, Chen, Shu, Wang, Xueting, Shen, Yanhua, Zeng, Ru, Wu, Qin, Lu, Yuanfu, Shi, Jingshan, Zhou, Shaoyu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8047462/
https://www.ncbi.nlm.nih.gov/pubmed/33889759
http://dx.doi.org/10.1016/j.bbrep.2020.100877
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author Fu, Xiaolong
Chen, Shu
Wang, Xueting
Shen, Yanhua
Zeng, Ru
Wu, Qin
Lu, Yuanfu
Shi, Jingshan
Zhou, Shaoyu
author_facet Fu, Xiaolong
Chen, Shu
Wang, Xueting
Shen, Yanhua
Zeng, Ru
Wu, Qin
Lu, Yuanfu
Shi, Jingshan
Zhou, Shaoyu
author_sort Fu, Xiaolong
collection PubMed
description Modern pharmacological studies have demonstrated that Dendrobium nobile Lindl. Alkaloids (DNLA), the main active ingredients of Dendrobium nobile, is valuable as an anti-aging and neuroprotective herbal medicine. The present study was designed to determine whether DNLA confers protective function over neurotoxicant manganese (Mn)-induced cytotoxicity and the mechanism involved. Our results showed that pretreatment of PC12 cells with DNLA alleviated cell toxicity induced by Mn and improved mitochondrial respiratory capacity and oxidative status. Mn treatment increased apoptotic cell death along with a marked increase in the protein expression of Bax and a decrease in the expression of Bcl-2 protein, all of which were noticeably reversed by DNLA. Furthermore, DNLA significantly abolished the decrease in protein levels of both PINK1 and Parkin, and mitigated the increased expression of autophagy marker LC3-II and accumulation of p62 caused by Mn. These results demonstrate that DNLA inhibits Mn induced cytotoxicity, which may be mediated through modulating PINK1/Parkin-mediated autophagic flux and improving mitochondrial function.
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spelling pubmed-80474622021-04-21 Dendrobium nobile Lindl. alkaloids alleviate Mn-induced neurotoxicity via PINK1/Parkin-mediated mitophagy in PC12 cells Fu, Xiaolong Chen, Shu Wang, Xueting Shen, Yanhua Zeng, Ru Wu, Qin Lu, Yuanfu Shi, Jingshan Zhou, Shaoyu Biochem Biophys Rep Research Article Modern pharmacological studies have demonstrated that Dendrobium nobile Lindl. Alkaloids (DNLA), the main active ingredients of Dendrobium nobile, is valuable as an anti-aging and neuroprotective herbal medicine. The present study was designed to determine whether DNLA confers protective function over neurotoxicant manganese (Mn)-induced cytotoxicity and the mechanism involved. Our results showed that pretreatment of PC12 cells with DNLA alleviated cell toxicity induced by Mn and improved mitochondrial respiratory capacity and oxidative status. Mn treatment increased apoptotic cell death along with a marked increase in the protein expression of Bax and a decrease in the expression of Bcl-2 protein, all of which were noticeably reversed by DNLA. Furthermore, DNLA significantly abolished the decrease in protein levels of both PINK1 and Parkin, and mitigated the increased expression of autophagy marker LC3-II and accumulation of p62 caused by Mn. These results demonstrate that DNLA inhibits Mn induced cytotoxicity, which may be mediated through modulating PINK1/Parkin-mediated autophagic flux and improving mitochondrial function. Elsevier 2021-04-02 /pmc/articles/PMC8047462/ /pubmed/33889759 http://dx.doi.org/10.1016/j.bbrep.2020.100877 Text en © 2020 Published by Elsevier B.V. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Fu, Xiaolong
Chen, Shu
Wang, Xueting
Shen, Yanhua
Zeng, Ru
Wu, Qin
Lu, Yuanfu
Shi, Jingshan
Zhou, Shaoyu
Dendrobium nobile Lindl. alkaloids alleviate Mn-induced neurotoxicity via PINK1/Parkin-mediated mitophagy in PC12 cells
title Dendrobium nobile Lindl. alkaloids alleviate Mn-induced neurotoxicity via PINK1/Parkin-mediated mitophagy in PC12 cells
title_full Dendrobium nobile Lindl. alkaloids alleviate Mn-induced neurotoxicity via PINK1/Parkin-mediated mitophagy in PC12 cells
title_fullStr Dendrobium nobile Lindl. alkaloids alleviate Mn-induced neurotoxicity via PINK1/Parkin-mediated mitophagy in PC12 cells
title_full_unstemmed Dendrobium nobile Lindl. alkaloids alleviate Mn-induced neurotoxicity via PINK1/Parkin-mediated mitophagy in PC12 cells
title_short Dendrobium nobile Lindl. alkaloids alleviate Mn-induced neurotoxicity via PINK1/Parkin-mediated mitophagy in PC12 cells
title_sort dendrobium nobile lindl. alkaloids alleviate mn-induced neurotoxicity via pink1/parkin-mediated mitophagy in pc12 cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8047462/
https://www.ncbi.nlm.nih.gov/pubmed/33889759
http://dx.doi.org/10.1016/j.bbrep.2020.100877
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