Cargando…
Pathological findings and morphologic correlation of the lungs of autopsied patients with SARS-CoV-2 infection in the Brazilian Amazon using transmission electron microscopy
INTRODUCTION: Electron microscopy (EM) is a rapid and effective tool that can be used to create images of a whole spectrum of virus-host interactions and, as such, has long been used in the discovery and description of viral mechanisms. METHODS: Electron microscopy was used to evaluate the pulmonary...
Autores principales: | , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Sociedade Brasileira de Medicina Tropical - SBMT
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8047700/ https://www.ncbi.nlm.nih.gov/pubmed/33886821 http://dx.doi.org/10.1590/0037-8682-0850-2020 |
_version_ | 1783679092913078272 |
---|---|
author | Santana, Monique Freire Pinto, Rebecca Augusta de Araújo Marcon, Bruna Hilzendeger de Medeiros, Lia Carolina Almeida Soares de Morais, Thiago Barros do Nascimento Dias, Lucas Castanhola de Souza, Lorenna Pereira de Melo, Gisely Cardoso Monteiro, Wuelton Marcelo Lacerda, Marcus Vinicius Guimarães Val, Fernando Almeida Lalwani, Pritesh Jaychand Ferreira, Luiz Carlos de Lima |
author_facet | Santana, Monique Freire Pinto, Rebecca Augusta de Araújo Marcon, Bruna Hilzendeger de Medeiros, Lia Carolina Almeida Soares de Morais, Thiago Barros do Nascimento Dias, Lucas Castanhola de Souza, Lorenna Pereira de Melo, Gisely Cardoso Monteiro, Wuelton Marcelo Lacerda, Marcus Vinicius Guimarães Val, Fernando Almeida Lalwani, Pritesh Jaychand Ferreira, Luiz Carlos de Lima |
author_sort | Santana, Monique Freire |
collection | PubMed |
description | INTRODUCTION: Electron microscopy (EM) is a rapid and effective tool that can be used to create images of a whole spectrum of virus-host interactions and, as such, has long been used in the discovery and description of viral mechanisms. METHODS: Electron microscopy was used to evaluate the pulmonary pathologies of postmortem lung sections from three patients who died from infection with SARS-associated coronavirus 2 (SARS-CoV-2), a new member of the Coronaviridae family. RESULTS: Diffuse alveolar damage (DAD) was predominant in all three patients. The early exudative stage was characterized principally by edema and extravasation of red blood cells into the alveolar space with injury to the alveolar epithelial cells; this was followed by detachment, apoptosis, and necrosis of type I and II pneumocytes. The capillaries exhibited congestion, exposure of the basement membrane from denuded endothelial cells, platelet adhesion, fibrin thrombi, and rupture of the capillary walls. The proliferative stage was characterized by pronounced proliferation of type II alveolar pneumocytes and multinucleated giant cells. The cytopathic effect of SARS-CoV-2 was observed both in degenerated type II pneumocytes and freely circulating in the alveoli, with components from virions, macrophages, lymphocytes, and cellular debris. CONCLUSIONS: Viral particles consistent with the characteristics of SARS-CoV-2 were observed mainly in degenerated pneumocytes, in the endothelium, or freely circulating in the alveoli. In the final stage of illness, the alveolar spaces were replaced by fibrosis. |
format | Online Article Text |
id | pubmed-8047700 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Sociedade Brasileira de Medicina Tropical - SBMT |
record_format | MEDLINE/PubMed |
spelling | pubmed-80477002021-04-16 Pathological findings and morphologic correlation of the lungs of autopsied patients with SARS-CoV-2 infection in the Brazilian Amazon using transmission electron microscopy Santana, Monique Freire Pinto, Rebecca Augusta de Araújo Marcon, Bruna Hilzendeger de Medeiros, Lia Carolina Almeida Soares de Morais, Thiago Barros do Nascimento Dias, Lucas Castanhola de Souza, Lorenna Pereira de Melo, Gisely Cardoso Monteiro, Wuelton Marcelo Lacerda, Marcus Vinicius Guimarães Val, Fernando Almeida Lalwani, Pritesh Jaychand Ferreira, Luiz Carlos de Lima Rev Soc Bras Med Trop Major Article INTRODUCTION: Electron microscopy (EM) is a rapid and effective tool that can be used to create images of a whole spectrum of virus-host interactions and, as such, has long been used in the discovery and description of viral mechanisms. METHODS: Electron microscopy was used to evaluate the pulmonary pathologies of postmortem lung sections from three patients who died from infection with SARS-associated coronavirus 2 (SARS-CoV-2), a new member of the Coronaviridae family. RESULTS: Diffuse alveolar damage (DAD) was predominant in all three patients. The early exudative stage was characterized principally by edema and extravasation of red blood cells into the alveolar space with injury to the alveolar epithelial cells; this was followed by detachment, apoptosis, and necrosis of type I and II pneumocytes. The capillaries exhibited congestion, exposure of the basement membrane from denuded endothelial cells, platelet adhesion, fibrin thrombi, and rupture of the capillary walls. The proliferative stage was characterized by pronounced proliferation of type II alveolar pneumocytes and multinucleated giant cells. The cytopathic effect of SARS-CoV-2 was observed both in degenerated type II pneumocytes and freely circulating in the alveoli, with components from virions, macrophages, lymphocytes, and cellular debris. CONCLUSIONS: Viral particles consistent with the characteristics of SARS-CoV-2 were observed mainly in degenerated pneumocytes, in the endothelium, or freely circulating in the alveoli. In the final stage of illness, the alveolar spaces were replaced by fibrosis. Sociedade Brasileira de Medicina Tropical - SBMT 2021-04-12 /pmc/articles/PMC8047700/ /pubmed/33886821 http://dx.doi.org/10.1590/0037-8682-0850-2020 Text en https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License |
spellingShingle | Major Article Santana, Monique Freire Pinto, Rebecca Augusta de Araújo Marcon, Bruna Hilzendeger de Medeiros, Lia Carolina Almeida Soares de Morais, Thiago Barros do Nascimento Dias, Lucas Castanhola de Souza, Lorenna Pereira de Melo, Gisely Cardoso Monteiro, Wuelton Marcelo Lacerda, Marcus Vinicius Guimarães Val, Fernando Almeida Lalwani, Pritesh Jaychand Ferreira, Luiz Carlos de Lima Pathological findings and morphologic correlation of the lungs of autopsied patients with SARS-CoV-2 infection in the Brazilian Amazon using transmission electron microscopy |
title | Pathological findings and morphologic correlation of the lungs of autopsied patients with SARS-CoV-2 infection in the Brazilian Amazon using transmission electron microscopy |
title_full | Pathological findings and morphologic correlation of the lungs of autopsied patients with SARS-CoV-2 infection in the Brazilian Amazon using transmission electron microscopy |
title_fullStr | Pathological findings and morphologic correlation of the lungs of autopsied patients with SARS-CoV-2 infection in the Brazilian Amazon using transmission electron microscopy |
title_full_unstemmed | Pathological findings and morphologic correlation of the lungs of autopsied patients with SARS-CoV-2 infection in the Brazilian Amazon using transmission electron microscopy |
title_short | Pathological findings and morphologic correlation of the lungs of autopsied patients with SARS-CoV-2 infection in the Brazilian Amazon using transmission electron microscopy |
title_sort | pathological findings and morphologic correlation of the lungs of autopsied patients with sars-cov-2 infection in the brazilian amazon using transmission electron microscopy |
topic | Major Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8047700/ https://www.ncbi.nlm.nih.gov/pubmed/33886821 http://dx.doi.org/10.1590/0037-8682-0850-2020 |
work_keys_str_mv | AT santanamoniquefreire pathologicalfindingsandmorphologiccorrelationofthelungsofautopsiedpatientswithsarscov2infectioninthebrazilianamazonusingtransmissionelectronmicroscopy AT pintorebeccaaugustadearaujo pathologicalfindingsandmorphologiccorrelationofthelungsofautopsiedpatientswithsarscov2infectioninthebrazilianamazonusingtransmissionelectronmicroscopy AT marconbrunahilzendeger pathologicalfindingsandmorphologiccorrelationofthelungsofautopsiedpatientswithsarscov2infectioninthebrazilianamazonusingtransmissionelectronmicroscopy AT demedeirosliacarolinaalmeidasoares pathologicalfindingsandmorphologiccorrelationofthelungsofautopsiedpatientswithsarscov2infectioninthebrazilianamazonusingtransmissionelectronmicroscopy AT demoraisthiagobarrosdonascimento pathologicalfindingsandmorphologiccorrelationofthelungsofautopsiedpatientswithsarscov2infectioninthebrazilianamazonusingtransmissionelectronmicroscopy AT diaslucascastanhola pathologicalfindingsandmorphologiccorrelationofthelungsofautopsiedpatientswithsarscov2infectioninthebrazilianamazonusingtransmissionelectronmicroscopy AT desouzalorennapereira pathologicalfindingsandmorphologiccorrelationofthelungsofautopsiedpatientswithsarscov2infectioninthebrazilianamazonusingtransmissionelectronmicroscopy AT demelogiselycardoso pathologicalfindingsandmorphologiccorrelationofthelungsofautopsiedpatientswithsarscov2infectioninthebrazilianamazonusingtransmissionelectronmicroscopy AT monteirowueltonmarcelo pathologicalfindingsandmorphologiccorrelationofthelungsofautopsiedpatientswithsarscov2infectioninthebrazilianamazonusingtransmissionelectronmicroscopy AT lacerdamarcusviniciusguimaraes pathologicalfindingsandmorphologiccorrelationofthelungsofautopsiedpatientswithsarscov2infectioninthebrazilianamazonusingtransmissionelectronmicroscopy AT valfernandoalmeida pathologicalfindingsandmorphologiccorrelationofthelungsofautopsiedpatientswithsarscov2infectioninthebrazilianamazonusingtransmissionelectronmicroscopy AT lalwanipriteshjaychand pathologicalfindingsandmorphologiccorrelationofthelungsofautopsiedpatientswithsarscov2infectioninthebrazilianamazonusingtransmissionelectronmicroscopy AT ferreiraluizcarlosdelima pathologicalfindingsandmorphologiccorrelationofthelungsofautopsiedpatientswithsarscov2infectioninthebrazilianamazonusingtransmissionelectronmicroscopy |