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Variation in Extracellular Polymeric Substances from Enterobacter sp. and Their Pb(2+) Adsorption Behaviors

[Image: see text] The objective of this study was to investigate the effects of the cultivation time, temperature, and pH value on the yield and composition of extracellular polymeric substances (EPS) from Enterobacter sp. FM-1 (FM-1) and to analyze the Pb(2+) adsorption behavior of soluble EPS (S-E...

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Autores principales: Li, Yi, Xin, Meifen, Xie, Dongyu, Fan, Shirui, Ma, Jiangming, Liu, Kehui, Yu, Fangming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2021
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8047720/
https://www.ncbi.nlm.nih.gov/pubmed/33869942
http://dx.doi.org/10.1021/acsomega.1c00185
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author Li, Yi
Xin, Meifen
Xie, Dongyu
Fan, Shirui
Ma, Jiangming
Liu, Kehui
Yu, Fangming
author_facet Li, Yi
Xin, Meifen
Xie, Dongyu
Fan, Shirui
Ma, Jiangming
Liu, Kehui
Yu, Fangming
author_sort Li, Yi
collection PubMed
description [Image: see text] The objective of this study was to investigate the effects of the cultivation time, temperature, and pH value on the yield and composition of extracellular polymeric substances (EPS) from Enterobacter sp. FM-1 (FM-1) and to analyze the Pb(2+) adsorption behavior of soluble EPS (S-EPS), loosely bound EPS (LB-EPS), and tightly bound EPS (TB-EPS). Maximum EPS production was obtained when the cultivation time, temperature, and pH value were 24 h, 30 °C, and 8.0, respectively. The main components of EPS were proteins, polysaccharides, and nucleic acids, but the different EPS types contained different proportions and specific components. The Pb(2+) adsorption capacity of LB-EPS was 2.23 and 1.50 times higher than that of S-EPS and TB-EPS, respectively. After Pb(2+) adsorption by LB-EPS, the pH value of the reaction system decreased to the lowest of 5.23, which indicated that LB-EPS contained more functional groups that could release H(+), which will help to better adsorb Pb(2+) through ion exchange. The three-dimensional excitation–emission matrix fluorescence spectroscopy (3D-EEM) analysis showed that the fluorescence intensity of tryptophan-containing substances decreased by 85.5% after Pb(2+) adsorption by LB-EPS, which indicated the complexation of tryptophan-containing substances with Pb(2+). Fourier transform infrared spectroscopy (FT-IR) and X-ray photoelectron spectroscopy (XPS) O spectra indicated that the C=O peak from protein amide I of tryptophan-containing substances in LB-EPS was mainly responsible for the complexation of Pb(2+). After the adsorption of Pb(2+), the proportion of the C=O peak in LB-EPS increased by 33.89%, indicating that the complexation of LB-EPS with Pb(2+) was mainly attributed to the O atom in the C=O terminus of protein amide I.
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spelling pubmed-80477202021-04-16 Variation in Extracellular Polymeric Substances from Enterobacter sp. and Their Pb(2+) Adsorption Behaviors Li, Yi Xin, Meifen Xie, Dongyu Fan, Shirui Ma, Jiangming Liu, Kehui Yu, Fangming ACS Omega [Image: see text] The objective of this study was to investigate the effects of the cultivation time, temperature, and pH value on the yield and composition of extracellular polymeric substances (EPS) from Enterobacter sp. FM-1 (FM-1) and to analyze the Pb(2+) adsorption behavior of soluble EPS (S-EPS), loosely bound EPS (LB-EPS), and tightly bound EPS (TB-EPS). Maximum EPS production was obtained when the cultivation time, temperature, and pH value were 24 h, 30 °C, and 8.0, respectively. The main components of EPS were proteins, polysaccharides, and nucleic acids, but the different EPS types contained different proportions and specific components. The Pb(2+) adsorption capacity of LB-EPS was 2.23 and 1.50 times higher than that of S-EPS and TB-EPS, respectively. After Pb(2+) adsorption by LB-EPS, the pH value of the reaction system decreased to the lowest of 5.23, which indicated that LB-EPS contained more functional groups that could release H(+), which will help to better adsorb Pb(2+) through ion exchange. The three-dimensional excitation–emission matrix fluorescence spectroscopy (3D-EEM) analysis showed that the fluorescence intensity of tryptophan-containing substances decreased by 85.5% after Pb(2+) adsorption by LB-EPS, which indicated the complexation of tryptophan-containing substances with Pb(2+). Fourier transform infrared spectroscopy (FT-IR) and X-ray photoelectron spectroscopy (XPS) O spectra indicated that the C=O peak from protein amide I of tryptophan-containing substances in LB-EPS was mainly responsible for the complexation of Pb(2+). After the adsorption of Pb(2+), the proportion of the C=O peak in LB-EPS increased by 33.89%, indicating that the complexation of LB-EPS with Pb(2+) was mainly attributed to the O atom in the C=O terminus of protein amide I. American Chemical Society 2021-04-01 /pmc/articles/PMC8047720/ /pubmed/33869942 http://dx.doi.org/10.1021/acsomega.1c00185 Text en © 2021 The Authors. Published by American Chemical Society Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Li, Yi
Xin, Meifen
Xie, Dongyu
Fan, Shirui
Ma, Jiangming
Liu, Kehui
Yu, Fangming
Variation in Extracellular Polymeric Substances from Enterobacter sp. and Their Pb(2+) Adsorption Behaviors
title Variation in Extracellular Polymeric Substances from Enterobacter sp. and Their Pb(2+) Adsorption Behaviors
title_full Variation in Extracellular Polymeric Substances from Enterobacter sp. and Their Pb(2+) Adsorption Behaviors
title_fullStr Variation in Extracellular Polymeric Substances from Enterobacter sp. and Their Pb(2+) Adsorption Behaviors
title_full_unstemmed Variation in Extracellular Polymeric Substances from Enterobacter sp. and Their Pb(2+) Adsorption Behaviors
title_short Variation in Extracellular Polymeric Substances from Enterobacter sp. and Their Pb(2+) Adsorption Behaviors
title_sort variation in extracellular polymeric substances from enterobacter sp. and their pb(2+) adsorption behaviors
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8047720/
https://www.ncbi.nlm.nih.gov/pubmed/33869942
http://dx.doi.org/10.1021/acsomega.1c00185
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