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Centrosome amplification mediates small extracellular vesicle secretion via lysosome disruption
Bidirectional communication between cells and their surrounding environment is critical in both normal and pathological settings. Extracellular vesicles (EVs), which facilitate the horizontal transfer of molecules between cells, are recognized as an important constituent of cell-cell communication....
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cell Press
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8047808/ https://www.ncbi.nlm.nih.gov/pubmed/33592190 http://dx.doi.org/10.1016/j.cub.2021.01.028 |
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author | Adams, Sophie D. Csere, Judit D’angelo, Gisela Carter, Edward P. Romao, Maryse Arnandis, Teresa Dodel, Martin Kocher, Hemant M. Grose, Richard Raposo, Graça Mardakheh, Faraz Godinho, Susana A. |
author_facet | Adams, Sophie D. Csere, Judit D’angelo, Gisela Carter, Edward P. Romao, Maryse Arnandis, Teresa Dodel, Martin Kocher, Hemant M. Grose, Richard Raposo, Graça Mardakheh, Faraz Godinho, Susana A. |
author_sort | Adams, Sophie D. |
collection | PubMed |
description | Bidirectional communication between cells and their surrounding environment is critical in both normal and pathological settings. Extracellular vesicles (EVs), which facilitate the horizontal transfer of molecules between cells, are recognized as an important constituent of cell-cell communication. In cancer, alterations in EV secretion contribute to the growth and metastasis of tumor cells. However, the mechanisms underlying these changes remain largely unknown. Here, we show that centrosome amplification is associated with and sufficient to promote small extracellular vesicle ((S)EV) secretion in pancreatic cancer cells. This is a direct result of lysosomal dysfunction, caused by increased reactive oxygen species (ROS) downstream of extra centrosomes. We propose that defects in lysosome function could promote multivesicular body fusion with the plasma membrane, thereby enhancing (S)EV secretion. Furthermore, we find that (S)EVs secreted in response to amplified centrosomes are functionally distinct and activate pancreatic stellate cells (PSCs). These activated PSCs promote the invasion of pancreatic cancer cells in heterotypic 3D cultures. We propose that (S)EVs secreted by cancer cells with amplified centrosomes influence the bidirectional communication between the tumor cells and the surrounding stroma to promote malignancy. |
format | Online Article Text |
id | pubmed-8047808 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Cell Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-80478082021-04-21 Centrosome amplification mediates small extracellular vesicle secretion via lysosome disruption Adams, Sophie D. Csere, Judit D’angelo, Gisela Carter, Edward P. Romao, Maryse Arnandis, Teresa Dodel, Martin Kocher, Hemant M. Grose, Richard Raposo, Graça Mardakheh, Faraz Godinho, Susana A. Curr Biol Article Bidirectional communication between cells and their surrounding environment is critical in both normal and pathological settings. Extracellular vesicles (EVs), which facilitate the horizontal transfer of molecules between cells, are recognized as an important constituent of cell-cell communication. In cancer, alterations in EV secretion contribute to the growth and metastasis of tumor cells. However, the mechanisms underlying these changes remain largely unknown. Here, we show that centrosome amplification is associated with and sufficient to promote small extracellular vesicle ((S)EV) secretion in pancreatic cancer cells. This is a direct result of lysosomal dysfunction, caused by increased reactive oxygen species (ROS) downstream of extra centrosomes. We propose that defects in lysosome function could promote multivesicular body fusion with the plasma membrane, thereby enhancing (S)EV secretion. Furthermore, we find that (S)EVs secreted in response to amplified centrosomes are functionally distinct and activate pancreatic stellate cells (PSCs). These activated PSCs promote the invasion of pancreatic cancer cells in heterotypic 3D cultures. We propose that (S)EVs secreted by cancer cells with amplified centrosomes influence the bidirectional communication between the tumor cells and the surrounding stroma to promote malignancy. Cell Press 2021-04-12 /pmc/articles/PMC8047808/ /pubmed/33592190 http://dx.doi.org/10.1016/j.cub.2021.01.028 Text en © 2021 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Adams, Sophie D. Csere, Judit D’angelo, Gisela Carter, Edward P. Romao, Maryse Arnandis, Teresa Dodel, Martin Kocher, Hemant M. Grose, Richard Raposo, Graça Mardakheh, Faraz Godinho, Susana A. Centrosome amplification mediates small extracellular vesicle secretion via lysosome disruption |
title | Centrosome amplification mediates small extracellular vesicle secretion via lysosome disruption |
title_full | Centrosome amplification mediates small extracellular vesicle secretion via lysosome disruption |
title_fullStr | Centrosome amplification mediates small extracellular vesicle secretion via lysosome disruption |
title_full_unstemmed | Centrosome amplification mediates small extracellular vesicle secretion via lysosome disruption |
title_short | Centrosome amplification mediates small extracellular vesicle secretion via lysosome disruption |
title_sort | centrosome amplification mediates small extracellular vesicle secretion via lysosome disruption |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8047808/ https://www.ncbi.nlm.nih.gov/pubmed/33592190 http://dx.doi.org/10.1016/j.cub.2021.01.028 |
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