Quantifying 35 transcripts in a single tube: model-based calibration of the GeXP multiplex RT-PCR assay

BACKGROUND: Quantitative analysis of differential gene expression is of central importance in molecular life sciences. The Gene eXpression Profiling technology (GeXP) relies on multiplex RT-PCR and subsequent capillary electrophoretic separation of the amplification products and allows to quantify t...

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Detalles Bibliográficos
Autores principales: Marquardt, Pauline, Werthmann, Britta, Rätzel, Viktoria, Haas, Markus, Marwan, Wolfgang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8048187/
https://www.ncbi.nlm.nih.gov/pubmed/33853570
http://dx.doi.org/10.1186/s12896-021-00689-4

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