Cargando…
Ca(2+)‐regulated cell migration revealed by optogenetically engineered Ca(2+) oscillations
The ability of a single Ca(2+) ion to play an important role in cell biology is highlighted by the need for cells to form Ca(2+) signals in the dimensions of space, time, and amplitude. Thus, spatial and temporal changes in intracellular Ca(2+) concentration are important for determining cell fate....
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8048425/ https://www.ncbi.nlm.nih.gov/pubmed/33244795 http://dx.doi.org/10.1002/jcp.30190 |
_version_ | 1783679217202888704 |
---|---|
author | Lai, Yi‐Shyun Chang, Ya‐Han Chen, Yong‐Yi Xu, Jixuan Yu, Chi‐Sian Chang, Su‐Jing Chen, Pai‐Sheng Tsai, Shaw‐Jenq Chiu, Wen‐Tai |
author_facet | Lai, Yi‐Shyun Chang, Ya‐Han Chen, Yong‐Yi Xu, Jixuan Yu, Chi‐Sian Chang, Su‐Jing Chen, Pai‐Sheng Tsai, Shaw‐Jenq Chiu, Wen‐Tai |
author_sort | Lai, Yi‐Shyun |
collection | PubMed |
description | The ability of a single Ca(2+) ion to play an important role in cell biology is highlighted by the need for cells to form Ca(2+) signals in the dimensions of space, time, and amplitude. Thus, spatial and temporal changes in intracellular Ca(2+) concentration are important for determining cell fate. Optogenetic technology has been developed to provide more precise and targeted stimulation of cells. Here, U2OS cells overexpressing Ca(2+) translocating channelrhodopsin (CatCh) were used to mediate Ca(2+) influx through blue light illumination with various parameters, such as intensity, frequency, duty cycle, and duration. We identified that several Ca(2+)‐dependent transcription factors and certain kinases can be activated by specific Ca(2+) waves. Using a wound‐healing assay, we found that low‐frequency Ca(2+) oscillations increased cell migration through the activation of NF‐κB. This study explores the regulation of cell migration by Ca(2+) signals. Thus, we can choose optical parameters to modulate Ca(2+) waves and achieve activation of specific signaling pathways. This novel methodology can be applied to clarify related cell‐signaling mechanisms in the future. |
format | Online Article Text |
id | pubmed-8048425 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-80484252021-04-16 Ca(2+)‐regulated cell migration revealed by optogenetically engineered Ca(2+) oscillations Lai, Yi‐Shyun Chang, Ya‐Han Chen, Yong‐Yi Xu, Jixuan Yu, Chi‐Sian Chang, Su‐Jing Chen, Pai‐Sheng Tsai, Shaw‐Jenq Chiu, Wen‐Tai J Cell Physiol Research Articles The ability of a single Ca(2+) ion to play an important role in cell biology is highlighted by the need for cells to form Ca(2+) signals in the dimensions of space, time, and amplitude. Thus, spatial and temporal changes in intracellular Ca(2+) concentration are important for determining cell fate. Optogenetic technology has been developed to provide more precise and targeted stimulation of cells. Here, U2OS cells overexpressing Ca(2+) translocating channelrhodopsin (CatCh) were used to mediate Ca(2+) influx through blue light illumination with various parameters, such as intensity, frequency, duty cycle, and duration. We identified that several Ca(2+)‐dependent transcription factors and certain kinases can be activated by specific Ca(2+) waves. Using a wound‐healing assay, we found that low‐frequency Ca(2+) oscillations increased cell migration through the activation of NF‐κB. This study explores the regulation of cell migration by Ca(2+) signals. Thus, we can choose optical parameters to modulate Ca(2+) waves and achieve activation of specific signaling pathways. This novel methodology can be applied to clarify related cell‐signaling mechanisms in the future. John Wiley and Sons Inc. 2020-11-26 2021-06 /pmc/articles/PMC8048425/ /pubmed/33244795 http://dx.doi.org/10.1002/jcp.30190 Text en © 2020 The Authors. Journal of Cellular Physiology Published by Wiley Periodicals, Inc. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Research Articles Lai, Yi‐Shyun Chang, Ya‐Han Chen, Yong‐Yi Xu, Jixuan Yu, Chi‐Sian Chang, Su‐Jing Chen, Pai‐Sheng Tsai, Shaw‐Jenq Chiu, Wen‐Tai Ca(2+)‐regulated cell migration revealed by optogenetically engineered Ca(2+) oscillations |
title | Ca(2+)‐regulated cell migration revealed by optogenetically engineered Ca(2+) oscillations |
title_full | Ca(2+)‐regulated cell migration revealed by optogenetically engineered Ca(2+) oscillations |
title_fullStr | Ca(2+)‐regulated cell migration revealed by optogenetically engineered Ca(2+) oscillations |
title_full_unstemmed | Ca(2+)‐regulated cell migration revealed by optogenetically engineered Ca(2+) oscillations |
title_short | Ca(2+)‐regulated cell migration revealed by optogenetically engineered Ca(2+) oscillations |
title_sort | ca(2+)‐regulated cell migration revealed by optogenetically engineered ca(2+) oscillations |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8048425/ https://www.ncbi.nlm.nih.gov/pubmed/33244795 http://dx.doi.org/10.1002/jcp.30190 |
work_keys_str_mv | AT laiyishyun ca2regulatedcellmigrationrevealedbyoptogeneticallyengineeredca2oscillations AT changyahan ca2regulatedcellmigrationrevealedbyoptogeneticallyengineeredca2oscillations AT chenyongyi ca2regulatedcellmigrationrevealedbyoptogeneticallyengineeredca2oscillations AT xujixuan ca2regulatedcellmigrationrevealedbyoptogeneticallyengineeredca2oscillations AT yuchisian ca2regulatedcellmigrationrevealedbyoptogeneticallyengineeredca2oscillations AT changsujing ca2regulatedcellmigrationrevealedbyoptogeneticallyengineeredca2oscillations AT chenpaisheng ca2regulatedcellmigrationrevealedbyoptogeneticallyengineeredca2oscillations AT tsaishawjenq ca2regulatedcellmigrationrevealedbyoptogeneticallyengineeredca2oscillations AT chiuwentai ca2regulatedcellmigrationrevealedbyoptogeneticallyengineeredca2oscillations |