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Ca(2+)‐regulated cell migration revealed by optogenetically engineered Ca(2+) oscillations

The ability of a single Ca(2+) ion to play an important role in cell biology is highlighted by the need for cells to form Ca(2+) signals in the dimensions of space, time, and amplitude. Thus, spatial and temporal changes in intracellular Ca(2+) concentration are important for determining cell fate....

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Autores principales: Lai, Yi‐Shyun, Chang, Ya‐Han, Chen, Yong‐Yi, Xu, Jixuan, Yu, Chi‐Sian, Chang, Su‐Jing, Chen, Pai‐Sheng, Tsai, Shaw‐Jenq, Chiu, Wen‐Tai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8048425/
https://www.ncbi.nlm.nih.gov/pubmed/33244795
http://dx.doi.org/10.1002/jcp.30190
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author Lai, Yi‐Shyun
Chang, Ya‐Han
Chen, Yong‐Yi
Xu, Jixuan
Yu, Chi‐Sian
Chang, Su‐Jing
Chen, Pai‐Sheng
Tsai, Shaw‐Jenq
Chiu, Wen‐Tai
author_facet Lai, Yi‐Shyun
Chang, Ya‐Han
Chen, Yong‐Yi
Xu, Jixuan
Yu, Chi‐Sian
Chang, Su‐Jing
Chen, Pai‐Sheng
Tsai, Shaw‐Jenq
Chiu, Wen‐Tai
author_sort Lai, Yi‐Shyun
collection PubMed
description The ability of a single Ca(2+) ion to play an important role in cell biology is highlighted by the need for cells to form Ca(2+) signals in the dimensions of space, time, and amplitude. Thus, spatial and temporal changes in intracellular Ca(2+) concentration are important for determining cell fate. Optogenetic technology has been developed to provide more precise and targeted stimulation of cells. Here, U2OS cells overexpressing Ca(2+) translocating channelrhodopsin (CatCh) were used to mediate Ca(2+) influx through blue light illumination with various parameters, such as intensity, frequency, duty cycle, and duration. We identified that several Ca(2+)‐dependent transcription factors and certain kinases can be activated by specific Ca(2+) waves. Using a wound‐healing assay, we found that low‐frequency Ca(2+) oscillations increased cell migration through the activation of NF‐κB. This study explores the regulation of cell migration by Ca(2+) signals. Thus, we can choose optical parameters to modulate Ca(2+) waves and achieve activation of specific signaling pathways. This novel methodology can be applied to clarify related cell‐signaling mechanisms in the future.
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spelling pubmed-80484252021-04-16 Ca(2+)‐regulated cell migration revealed by optogenetically engineered Ca(2+) oscillations Lai, Yi‐Shyun Chang, Ya‐Han Chen, Yong‐Yi Xu, Jixuan Yu, Chi‐Sian Chang, Su‐Jing Chen, Pai‐Sheng Tsai, Shaw‐Jenq Chiu, Wen‐Tai J Cell Physiol Research Articles The ability of a single Ca(2+) ion to play an important role in cell biology is highlighted by the need for cells to form Ca(2+) signals in the dimensions of space, time, and amplitude. Thus, spatial and temporal changes in intracellular Ca(2+) concentration are important for determining cell fate. Optogenetic technology has been developed to provide more precise and targeted stimulation of cells. Here, U2OS cells overexpressing Ca(2+) translocating channelrhodopsin (CatCh) were used to mediate Ca(2+) influx through blue light illumination with various parameters, such as intensity, frequency, duty cycle, and duration. We identified that several Ca(2+)‐dependent transcription factors and certain kinases can be activated by specific Ca(2+) waves. Using a wound‐healing assay, we found that low‐frequency Ca(2+) oscillations increased cell migration through the activation of NF‐κB. This study explores the regulation of cell migration by Ca(2+) signals. Thus, we can choose optical parameters to modulate Ca(2+) waves and achieve activation of specific signaling pathways. This novel methodology can be applied to clarify related cell‐signaling mechanisms in the future. John Wiley and Sons Inc. 2020-11-26 2021-06 /pmc/articles/PMC8048425/ /pubmed/33244795 http://dx.doi.org/10.1002/jcp.30190 Text en © 2020 The Authors. Journal of Cellular Physiology Published by Wiley Periodicals, Inc. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Research Articles
Lai, Yi‐Shyun
Chang, Ya‐Han
Chen, Yong‐Yi
Xu, Jixuan
Yu, Chi‐Sian
Chang, Su‐Jing
Chen, Pai‐Sheng
Tsai, Shaw‐Jenq
Chiu, Wen‐Tai
Ca(2+)‐regulated cell migration revealed by optogenetically engineered Ca(2+) oscillations
title Ca(2+)‐regulated cell migration revealed by optogenetically engineered Ca(2+) oscillations
title_full Ca(2+)‐regulated cell migration revealed by optogenetically engineered Ca(2+) oscillations
title_fullStr Ca(2+)‐regulated cell migration revealed by optogenetically engineered Ca(2+) oscillations
title_full_unstemmed Ca(2+)‐regulated cell migration revealed by optogenetically engineered Ca(2+) oscillations
title_short Ca(2+)‐regulated cell migration revealed by optogenetically engineered Ca(2+) oscillations
title_sort ca(2+)‐regulated cell migration revealed by optogenetically engineered ca(2+) oscillations
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8048425/
https://www.ncbi.nlm.nih.gov/pubmed/33244795
http://dx.doi.org/10.1002/jcp.30190
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