Synthesis of [4S‐(2)H]NADH, [4R‐(2)H]NADH, [4‐(2)H(2)]NADH and [4‐(2)H]NAD(+) cofactors through heterogeneous biocatalysis in heavy water

This practitioner protocol describes the synthesis of a family of deuterated nicotinamide cofactors: [4S‐(2)H]NADH, [4R‐(2)H]NADH, [4‐(2)H(2)]NADH and [4‐(2)H]NAD(+). The application of a recently developed H(2)‐driven heterogeneous biocatalyst enables the cofactors to be prepared with high (>90%...

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Detalles Bibliográficos
Autores principales: Rowbotham, Jack S., Hardy, Adam P., Reeve, Holly A., Vincent, Kylie A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Practitioner Protocol ‐ Synthesis
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8048645/
https://www.ncbi.nlm.nih.gov/pubmed/33497029
http://dx.doi.org/10.1002/jlcr.3899
Descripción
Sumario:This practitioner protocol describes the synthesis of a family of deuterated nicotinamide cofactors: [4S‐(2)H]NADH, [4R‐(2)H]NADH, [4‐(2)H(2)]NADH and [4‐(2)H]NAD(+). The application of a recently developed H(2)‐driven heterogeneous biocatalyst enables the cofactors to be prepared with high (>90%) (2)H‐incorporation with (2)H(2)O as the only isotope source.

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