Spectrum‐effect relationship between UPLC fingerprints and anti‐lung cancer effect of Panax ginseng

OBJECTIVES: Lung cancer has the highest mortality rate among the various types of cancer. Panax ginseng (C. A. Mey). is a popular anti‐cancer herbal supplement. The quality control of ginseng is crucial to ensure its clinical efficacy. This study aimed to establish new quality control methods for ginseng and to identify its main active components responsible for lung cancer treatment. METHODS: Ultra‐high‐performance liquid chromatography (UPLC) was used to establish fingerprints of 18 batches of ginseng. CCK‐8 test was performed to evaluate the inhibitory activity of ginseng on Lewis lung cancer (LLC) cells. The spectrum‐effect relationship analysis of ginseng was assessed by canonical correlation analysis (CCA) and bioactivity validation. KEY FINDINGS: Six common peaks were identified and the variation coefficients were determined. The 18 batches of ginseng inhibited the proliferation of LLC cells to different degrees, showing different half maximal inhibitory concentration (IC(50)) values. Spectrum‐effect relationship analysis showed that ginsenoside Ro is the main anti‐proliferative constituent of LLC cell. CONCLUSIONS: Spectrum‐effect relationship is suitable for quality control of ginseng used for lung cancer. It is also effective in discovering the active ingredients related to the clinical efficacy of traditional Chinese medicine.