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HIV-1(LAI) Nef blocks the development of hematopoietic stem/progenitor cells into T lymphoid cells
OBJECTIVE: Despite successful antiviral therapy, the recovery of CD4(+) T cells may not be complete in certain HIV-1-infected individuals. In our previous work with humanized mice infected with CXCR4-tropic HIV-1(LAI) (LAI), viral protein Nef was found the major factor determining rapid loss of both...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Lippincott Williams & Wilkins
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8048728/ https://www.ncbi.nlm.nih.gov/pubmed/33587447 http://dx.doi.org/10.1097/QAD.0000000000002837 |
Sumario: | OBJECTIVE: Despite successful antiviral therapy, the recovery of CD4(+) T cells may not be complete in certain HIV-1-infected individuals. In our previous work with humanized mice infected with CXCR4-tropic HIV-1(LAI) (LAI), viral protein Nef was found the major factor determining rapid loss of both CD4(+) T cells and CD4(+)CD8(+) thymocytes but its effect on early T-cell development is unknown. The objective of this study is to investigate the influence of LAI Nef on the development of hematopoietic stem/progenitor cells (HSPCs) into T lymphoid cells. DESIGN: HSPC-OP9-DL1 cell co-culture and humanized mouse model was used to investigate the objective of our study in vitro and in vivo. RNA-seq was exploited to study the change of gene expression signature after nef expression in HSPCs. RESULTS: Nef expression in HSPCs was found to block their development into T lymphoid cells both in vitro and in the mice reconstituted with nef-expressing HSPCs derived from human cord blood. More surprisingly, in humanized mice nef expression preferentially suppressed the production of CD4(+) T cells. This developmental defect was not the result of CD34+ cell loss. RNA-seq analysis revealed that Nef affected the expression of 176 genes in HSPCs, including those involved in tumor necrosis factor, Toll-like receptor, and nucleotide-binding oligomerization domain-like receptor signaling pathways that are important for hematopoietic cell development. CONCLUSION: Our results demonstrate that Nef compromises the development of HSPCs into T lymphoid cells, especially CD4(+) T cells. This observation suggests that therapeutics targeting Nef may correct HIV-1-associated hematopoietic abnormalities, especially defects in T-cell development. |
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