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Kinetics of Muscle Carnosine Decay after β-Alanine Supplementation: A 16-wk Washout Study
PURPOSE: This study aimed to describe the kinetics of carnosine washout in human skeletal muscle over 16 wk. METHODS: Carnosine washout kinetics were studied in 15 young, physically active omnivorous men randomly assigned to take 6.4 g·d(−1) of β-alanine (n = 11) or placebo (n = 4) for 8 wk. Muscle...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Lippincott Williams & Wilkins
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8048732/ https://www.ncbi.nlm.nih.gov/pubmed/33148972 http://dx.doi.org/10.1249/MSS.0000000000002559 |
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author | YAMAGUCHI, GUILHERME CARVALHO NEMEZIO, KLEINER SCHULZ, MARIANE LEICHSENRING NATALI, JOSÉ CESAR, JONATAS EDUARDO RIANI, LUIZ AUGUSTO GONÇALVES, LÍVIA DE SOUZA MÖLLER, GABRIELLA BERWIG SALE, CRAIG DE MEDEIROS, MARISA HELENA GENNARI GUALANO, BRUNO ARTIOLI, GUILHERME GIANNINI |
author_facet | YAMAGUCHI, GUILHERME CARVALHO NEMEZIO, KLEINER SCHULZ, MARIANE LEICHSENRING NATALI, JOSÉ CESAR, JONATAS EDUARDO RIANI, LUIZ AUGUSTO GONÇALVES, LÍVIA DE SOUZA MÖLLER, GABRIELLA BERWIG SALE, CRAIG DE MEDEIROS, MARISA HELENA GENNARI GUALANO, BRUNO ARTIOLI, GUILHERME GIANNINI |
author_sort | YAMAGUCHI, GUILHERME CARVALHO |
collection | PubMed |
description | PURPOSE: This study aimed to describe the kinetics of carnosine washout in human skeletal muscle over 16 wk. METHODS: Carnosine washout kinetics were studied in 15 young, physically active omnivorous men randomly assigned to take 6.4 g·d(−1) of β-alanine (n = 11) or placebo (n = 4) for 8 wk. Muscle carnosine content (M-Carn) was determined before (PRE), immediately after (POST), and 4, 8, 12, and 16 wk after supplementation. High-intensity exercise tests were performed at these same time points. Linear and exponential models were fitted to the washout data, and the leave-one-out method was used to select the model with the best fit for M-Carn decay data. Repeated-measures correlation analysis was used to assess the association between changes in M-Carn and changes in performance. RESULTS: M-Carn increased from PRE to POST in the β-alanine group only (+91.1% ± 29.1%; placebo, +0.04% ± 10.1%; P < 0.0001). M-Carn started to decrease after cessation of β-alanine supplementation and continued to decrease until week 16 (POST4, +59% ± 40%; POST8, +35% ± 39%; POST12, +18% ± 32%; POST16, −3% ± 24% of PRE M-Carn). From week 12 onward, M-Carn was no longer statistically different from PRE. Both linear and exponential models displayed very similar fit and could be used to describe carnosine washout, although the linear model presented a slightly better fit. The decay in M-Carn was mirrored by a similar decay in high-intensity exercise tolerance; M-Carn was moderately and significantly correlated with total mechanical work done (r = 0.505; P = 0.032) and time to exhaustion (r = 0.72; P < 0.001). CONCLUSIONS: Carnosine washout takes 12–16 wk to complete, and it can be described either by linear or exponential curves. Changes in M-Carn seem to be mirrored by changes in high-intensity exercise tolerance. This information can be used to optimize β-alanine supplementation strategies. |
format | Online Article Text |
id | pubmed-8048732 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Lippincott Williams & Wilkins |
record_format | MEDLINE/PubMed |
spelling | pubmed-80487322021-04-19 Kinetics of Muscle Carnosine Decay after β-Alanine Supplementation: A 16-wk Washout Study YAMAGUCHI, GUILHERME CARVALHO NEMEZIO, KLEINER SCHULZ, MARIANE LEICHSENRING NATALI, JOSÉ CESAR, JONATAS EDUARDO RIANI, LUIZ AUGUSTO GONÇALVES, LÍVIA DE SOUZA MÖLLER, GABRIELLA BERWIG SALE, CRAIG DE MEDEIROS, MARISA HELENA GENNARI GUALANO, BRUNO ARTIOLI, GUILHERME GIANNINI Med Sci Sports Exerc Applied Sciences PURPOSE: This study aimed to describe the kinetics of carnosine washout in human skeletal muscle over 16 wk. METHODS: Carnosine washout kinetics were studied in 15 young, physically active omnivorous men randomly assigned to take 6.4 g·d(−1) of β-alanine (n = 11) or placebo (n = 4) for 8 wk. Muscle carnosine content (M-Carn) was determined before (PRE), immediately after (POST), and 4, 8, 12, and 16 wk after supplementation. High-intensity exercise tests were performed at these same time points. Linear and exponential models were fitted to the washout data, and the leave-one-out method was used to select the model with the best fit for M-Carn decay data. Repeated-measures correlation analysis was used to assess the association between changes in M-Carn and changes in performance. RESULTS: M-Carn increased from PRE to POST in the β-alanine group only (+91.1% ± 29.1%; placebo, +0.04% ± 10.1%; P < 0.0001). M-Carn started to decrease after cessation of β-alanine supplementation and continued to decrease until week 16 (POST4, +59% ± 40%; POST8, +35% ± 39%; POST12, +18% ± 32%; POST16, −3% ± 24% of PRE M-Carn). From week 12 onward, M-Carn was no longer statistically different from PRE. Both linear and exponential models displayed very similar fit and could be used to describe carnosine washout, although the linear model presented a slightly better fit. The decay in M-Carn was mirrored by a similar decay in high-intensity exercise tolerance; M-Carn was moderately and significantly correlated with total mechanical work done (r = 0.505; P = 0.032) and time to exhaustion (r = 0.72; P < 0.001). CONCLUSIONS: Carnosine washout takes 12–16 wk to complete, and it can be described either by linear or exponential curves. Changes in M-Carn seem to be mirrored by changes in high-intensity exercise tolerance. This information can be used to optimize β-alanine supplementation strategies. Lippincott Williams & Wilkins 2021-05 2020-11-04 /pmc/articles/PMC8048732/ /pubmed/33148972 http://dx.doi.org/10.1249/MSS.0000000000002559 Text en Copyright © 2020 The Author(s). Published by Wolters Kluwer Health, Inc. on behalf of the American College of Sports Medicine. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND) (https://creativecommons.org/licenses/by-nc-nd/4.0/) , where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal. |
spellingShingle | Applied Sciences YAMAGUCHI, GUILHERME CARVALHO NEMEZIO, KLEINER SCHULZ, MARIANE LEICHSENRING NATALI, JOSÉ CESAR, JONATAS EDUARDO RIANI, LUIZ AUGUSTO GONÇALVES, LÍVIA DE SOUZA MÖLLER, GABRIELLA BERWIG SALE, CRAIG DE MEDEIROS, MARISA HELENA GENNARI GUALANO, BRUNO ARTIOLI, GUILHERME GIANNINI Kinetics of Muscle Carnosine Decay after β-Alanine Supplementation: A 16-wk Washout Study |
title | Kinetics of Muscle Carnosine Decay after β-Alanine Supplementation: A 16-wk Washout Study |
title_full | Kinetics of Muscle Carnosine Decay after β-Alanine Supplementation: A 16-wk Washout Study |
title_fullStr | Kinetics of Muscle Carnosine Decay after β-Alanine Supplementation: A 16-wk Washout Study |
title_full_unstemmed | Kinetics of Muscle Carnosine Decay after β-Alanine Supplementation: A 16-wk Washout Study |
title_short | Kinetics of Muscle Carnosine Decay after β-Alanine Supplementation: A 16-wk Washout Study |
title_sort | kinetics of muscle carnosine decay after β-alanine supplementation: a 16-wk washout study |
topic | Applied Sciences |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8048732/ https://www.ncbi.nlm.nih.gov/pubmed/33148972 http://dx.doi.org/10.1249/MSS.0000000000002559 |
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