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HIGH RESOLUTION MASS SPECTROMETRY IN LIPIDOMICS

The boost of research output in lipidomics during the last decade is tightly linked to improved instrumentation in mass spectrometry. Associated with this trend is the shift from low resolution—toward high‐resolution lipidomics platforms. This review article summarizes the state of the art in the li...

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Detalles Bibliográficos
Autores principales: Züllig, Thomas, Köfeler, Harald C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8049033/
https://www.ncbi.nlm.nih.gov/pubmed/32233039
http://dx.doi.org/10.1002/mas.21627
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author Züllig, Thomas
Köfeler, Harald C.
author_facet Züllig, Thomas
Köfeler, Harald C.
author_sort Züllig, Thomas
collection PubMed
description The boost of research output in lipidomics during the last decade is tightly linked to improved instrumentation in mass spectrometry. Associated with this trend is the shift from low resolution—toward high‐resolution lipidomics platforms. This review article summarizes the state of the art in the lipidomics field with a particular focus on the merits of high mass resolution. Following some theoretical considerations on the benefits of high mass resolution in lipidomics, it starts with a historical perspective on lipid analysis by sector instruments and moves further to today's instrumental approaches, including shotgun lipidomics, liquid chromatography–mass spectrometry, matrix‐assisted laser desorption ionization‐time‐of‐flight, and imaging lipidomics. Subsequently, several data processing and data analysis software packages are critically evaluated with all their pros and cons. Finally, this article emphasizes the importance and necessity of quality standards as the field evolves from its pioneering phase into a mature and robust omics technology and lists various initiatives for improving the applicability of lipidomics. © 2020 The Authors. Mass Spectrometry Reviews published by John Wiley & Sons Ltd. Mass Spec Rev
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spelling pubmed-80490332021-04-20 HIGH RESOLUTION MASS SPECTROMETRY IN LIPIDOMICS Züllig, Thomas Köfeler, Harald C. Mass Spectrom Rev Review Articles The boost of research output in lipidomics during the last decade is tightly linked to improved instrumentation in mass spectrometry. Associated with this trend is the shift from low resolution—toward high‐resolution lipidomics platforms. This review article summarizes the state of the art in the lipidomics field with a particular focus on the merits of high mass resolution. Following some theoretical considerations on the benefits of high mass resolution in lipidomics, it starts with a historical perspective on lipid analysis by sector instruments and moves further to today's instrumental approaches, including shotgun lipidomics, liquid chromatography–mass spectrometry, matrix‐assisted laser desorption ionization‐time‐of‐flight, and imaging lipidomics. Subsequently, several data processing and data analysis software packages are critically evaluated with all their pros and cons. Finally, this article emphasizes the importance and necessity of quality standards as the field evolves from its pioneering phase into a mature and robust omics technology and lists various initiatives for improving the applicability of lipidomics. © 2020 The Authors. Mass Spectrometry Reviews published by John Wiley & Sons Ltd. Mass Spec Rev John Wiley and Sons Inc. 2020-03-31 2021 /pmc/articles/PMC8049033/ /pubmed/32233039 http://dx.doi.org/10.1002/mas.21627 Text en © 2020 The Authors. Mass Spectrometry Reviews published by John Wiley & Sons Ltd https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Review Articles
Züllig, Thomas
Köfeler, Harald C.
HIGH RESOLUTION MASS SPECTROMETRY IN LIPIDOMICS
title HIGH RESOLUTION MASS SPECTROMETRY IN LIPIDOMICS
title_full HIGH RESOLUTION MASS SPECTROMETRY IN LIPIDOMICS
title_fullStr HIGH RESOLUTION MASS SPECTROMETRY IN LIPIDOMICS
title_full_unstemmed HIGH RESOLUTION MASS SPECTROMETRY IN LIPIDOMICS
title_short HIGH RESOLUTION MASS SPECTROMETRY IN LIPIDOMICS
title_sort high resolution mass spectrometry in lipidomics
topic Review Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8049033/
https://www.ncbi.nlm.nih.gov/pubmed/32233039
http://dx.doi.org/10.1002/mas.21627
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