Cargando…

Two transcriptionally distinct pathways drive female development in a reptile with both genetic and temperature dependent sex determination

How temperature determines sex remains unknown. A recent hypothesis proposes that conserved cellular mechanisms (calcium and redox; ‘CaRe’ status) sense temperature and identify genes and regulatory pathways likely to be involved in driving sexual development. We take advantage of the unique sex det...

Descripción completa

Detalles Bibliográficos
Autores principales: Whiteley, Sarah L., Holleley, Clare E., Wagner, Susan, Blackburn, James, Deveson, Ira W., Marshall Graves, Jennifer A., Georges, Arthur
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8049264/
https://www.ncbi.nlm.nih.gov/pubmed/33857129
http://dx.doi.org/10.1371/journal.pgen.1009465
_version_ 1783679396108828672
author Whiteley, Sarah L.
Holleley, Clare E.
Wagner, Susan
Blackburn, James
Deveson, Ira W.
Marshall Graves, Jennifer A.
Georges, Arthur
author_facet Whiteley, Sarah L.
Holleley, Clare E.
Wagner, Susan
Blackburn, James
Deveson, Ira W.
Marshall Graves, Jennifer A.
Georges, Arthur
author_sort Whiteley, Sarah L.
collection PubMed
description How temperature determines sex remains unknown. A recent hypothesis proposes that conserved cellular mechanisms (calcium and redox; ‘CaRe’ status) sense temperature and identify genes and regulatory pathways likely to be involved in driving sexual development. We take advantage of the unique sex determining system of the model organism, Pogona vitticeps, to assess predictions of this hypothesis. P. vitticeps has ZZ male: ZW female sex chromosomes whose influence can be overridden in genetic males by high temperatures, causing male-to-female sex reversal. We compare a developmental transcriptome series of ZWf females and temperature sex reversed ZZf females. We demonstrate that early developmental cascades differ dramatically between genetically driven and thermally driven females, later converging to produce a common outcome (ovaries). We show that genes proposed as regulators of thermosensitive sex determination play a role in temperature sex reversal. Our study greatly advances the search for the mechanisms by which temperature determines sex.
format Online
Article
Text
id pubmed-8049264
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-80492642021-04-21 Two transcriptionally distinct pathways drive female development in a reptile with both genetic and temperature dependent sex determination Whiteley, Sarah L. Holleley, Clare E. Wagner, Susan Blackburn, James Deveson, Ira W. Marshall Graves, Jennifer A. Georges, Arthur PLoS Genet Research Article How temperature determines sex remains unknown. A recent hypothesis proposes that conserved cellular mechanisms (calcium and redox; ‘CaRe’ status) sense temperature and identify genes and regulatory pathways likely to be involved in driving sexual development. We take advantage of the unique sex determining system of the model organism, Pogona vitticeps, to assess predictions of this hypothesis. P. vitticeps has ZZ male: ZW female sex chromosomes whose influence can be overridden in genetic males by high temperatures, causing male-to-female sex reversal. We compare a developmental transcriptome series of ZWf females and temperature sex reversed ZZf females. We demonstrate that early developmental cascades differ dramatically between genetically driven and thermally driven females, later converging to produce a common outcome (ovaries). We show that genes proposed as regulators of thermosensitive sex determination play a role in temperature sex reversal. Our study greatly advances the search for the mechanisms by which temperature determines sex. Public Library of Science 2021-04-15 /pmc/articles/PMC8049264/ /pubmed/33857129 http://dx.doi.org/10.1371/journal.pgen.1009465 Text en © 2021 Whiteley et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Whiteley, Sarah L.
Holleley, Clare E.
Wagner, Susan
Blackburn, James
Deveson, Ira W.
Marshall Graves, Jennifer A.
Georges, Arthur
Two transcriptionally distinct pathways drive female development in a reptile with both genetic and temperature dependent sex determination
title Two transcriptionally distinct pathways drive female development in a reptile with both genetic and temperature dependent sex determination
title_full Two transcriptionally distinct pathways drive female development in a reptile with both genetic and temperature dependent sex determination
title_fullStr Two transcriptionally distinct pathways drive female development in a reptile with both genetic and temperature dependent sex determination
title_full_unstemmed Two transcriptionally distinct pathways drive female development in a reptile with both genetic and temperature dependent sex determination
title_short Two transcriptionally distinct pathways drive female development in a reptile with both genetic and temperature dependent sex determination
title_sort two transcriptionally distinct pathways drive female development in a reptile with both genetic and temperature dependent sex determination
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8049264/
https://www.ncbi.nlm.nih.gov/pubmed/33857129
http://dx.doi.org/10.1371/journal.pgen.1009465
work_keys_str_mv AT whiteleysarahl twotranscriptionallydistinctpathwaysdrivefemaledevelopmentinareptilewithbothgeneticandtemperaturedependentsexdetermination
AT holleleyclaree twotranscriptionallydistinctpathwaysdrivefemaledevelopmentinareptilewithbothgeneticandtemperaturedependentsexdetermination
AT wagnersusan twotranscriptionallydistinctpathwaysdrivefemaledevelopmentinareptilewithbothgeneticandtemperaturedependentsexdetermination
AT blackburnjames twotranscriptionallydistinctpathwaysdrivefemaledevelopmentinareptilewithbothgeneticandtemperaturedependentsexdetermination
AT devesoniraw twotranscriptionallydistinctpathwaysdrivefemaledevelopmentinareptilewithbothgeneticandtemperaturedependentsexdetermination
AT marshallgravesjennifera twotranscriptionallydistinctpathwaysdrivefemaledevelopmentinareptilewithbothgeneticandtemperaturedependentsexdetermination
AT georgesarthur twotranscriptionallydistinctpathwaysdrivefemaledevelopmentinareptilewithbothgeneticandtemperaturedependentsexdetermination