Cargando…
Proteomic Responses to Alkali Stress in Oats and the Alleviatory Effects of Exogenous Spermine Application
Alkali stress limits plant growth and yield more strongly than salt stress and can lead to the appearance of yellow leaves; however, the reasons remain unclear. In this study, we found that (1) the down-regulation of coproporphyrinogen III oxidase, protoporphyrinogen oxidase, and Pheophorbide a oxyg...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8049610/ https://www.ncbi.nlm.nih.gov/pubmed/33868329 http://dx.doi.org/10.3389/fpls.2021.627129 |
_version_ | 1783679451297480704 |
---|---|
author | Bai, Jianhui Jin, Ke Qin, Wei Wang, Yuqing Yin, Qiang |
author_facet | Bai, Jianhui Jin, Ke Qin, Wei Wang, Yuqing Yin, Qiang |
author_sort | Bai, Jianhui |
collection | PubMed |
description | Alkali stress limits plant growth and yield more strongly than salt stress and can lead to the appearance of yellow leaves; however, the reasons remain unclear. In this study, we found that (1) the down-regulation of coproporphyrinogen III oxidase, protoporphyrinogen oxidase, and Pheophorbide a oxygenase in oats under alkali stress contributes to the appearance of yellow leaves (as assessed by proteome and western blot analyses). (2) Some oat proteins that are involved in the antioxidant system, root growth, and jasmonic acid (JA) and indole-3-acetic acid (IAA) synthesis are up-regulated in response to alkalinity and help increase alkali tolerance. (3) We added exogenous spermine to oat plants to improve their alkali tolerance, which resulted in higher chlorophyll contents and plant dry weights than in plants subjected to alkaline stress alone. This was due to up-regulation of chitinase and proteins related to chloroplast structure, root growth, and the antioxidant system. Spermine addition increased sucrose utilization efficiency, and promoted carbohydrate export from leaves to roots to increase energy storage in roots. Spermine addition also increased the IAA and JA contents required for root growth. |
format | Online Article Text |
id | pubmed-8049610 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-80496102021-04-16 Proteomic Responses to Alkali Stress in Oats and the Alleviatory Effects of Exogenous Spermine Application Bai, Jianhui Jin, Ke Qin, Wei Wang, Yuqing Yin, Qiang Front Plant Sci Plant Science Alkali stress limits plant growth and yield more strongly than salt stress and can lead to the appearance of yellow leaves; however, the reasons remain unclear. In this study, we found that (1) the down-regulation of coproporphyrinogen III oxidase, protoporphyrinogen oxidase, and Pheophorbide a oxygenase in oats under alkali stress contributes to the appearance of yellow leaves (as assessed by proteome and western blot analyses). (2) Some oat proteins that are involved in the antioxidant system, root growth, and jasmonic acid (JA) and indole-3-acetic acid (IAA) synthesis are up-regulated in response to alkalinity and help increase alkali tolerance. (3) We added exogenous spermine to oat plants to improve their alkali tolerance, which resulted in higher chlorophyll contents and plant dry weights than in plants subjected to alkaline stress alone. This was due to up-regulation of chitinase and proteins related to chloroplast structure, root growth, and the antioxidant system. Spermine addition increased sucrose utilization efficiency, and promoted carbohydrate export from leaves to roots to increase energy storage in roots. Spermine addition also increased the IAA and JA contents required for root growth. Frontiers Media S.A. 2021-04-01 /pmc/articles/PMC8049610/ /pubmed/33868329 http://dx.doi.org/10.3389/fpls.2021.627129 Text en Copyright © 2021 Bai, Jin, Qin, Wang and Yin. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Bai, Jianhui Jin, Ke Qin, Wei Wang, Yuqing Yin, Qiang Proteomic Responses to Alkali Stress in Oats and the Alleviatory Effects of Exogenous Spermine Application |
title | Proteomic Responses to Alkali Stress in Oats and the Alleviatory Effects of Exogenous Spermine Application |
title_full | Proteomic Responses to Alkali Stress in Oats and the Alleviatory Effects of Exogenous Spermine Application |
title_fullStr | Proteomic Responses to Alkali Stress in Oats and the Alleviatory Effects of Exogenous Spermine Application |
title_full_unstemmed | Proteomic Responses to Alkali Stress in Oats and the Alleviatory Effects of Exogenous Spermine Application |
title_short | Proteomic Responses to Alkali Stress in Oats and the Alleviatory Effects of Exogenous Spermine Application |
title_sort | proteomic responses to alkali stress in oats and the alleviatory effects of exogenous spermine application |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8049610/ https://www.ncbi.nlm.nih.gov/pubmed/33868329 http://dx.doi.org/10.3389/fpls.2021.627129 |
work_keys_str_mv | AT baijianhui proteomicresponsestoalkalistressinoatsandthealleviatoryeffectsofexogenousspermineapplication AT jinke proteomicresponsestoalkalistressinoatsandthealleviatoryeffectsofexogenousspermineapplication AT qinwei proteomicresponsestoalkalistressinoatsandthealleviatoryeffectsofexogenousspermineapplication AT wangyuqing proteomicresponsestoalkalistressinoatsandthealleviatoryeffectsofexogenousspermineapplication AT yinqiang proteomicresponsestoalkalistressinoatsandthealleviatoryeffectsofexogenousspermineapplication |