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Antigen tests for COVID-19
PCR diagnosis has been considered as the gold standard for coronavirus disease 2019 (COVID-19) and other many diseases. However, there are many problems in using PCR, such as non-specific (i.e., false-positive) and false-negative amplifications, the limits of a target sample volume, deactivation of...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Biophysical Society of Japan
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8049777/ https://www.ncbi.nlm.nih.gov/pubmed/33954080 http://dx.doi.org/10.2142/biophysico.bppb-v18.004 |
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author | Kyosei, Yuta Yamura, Sou Namba, Mayuri Yoshimura, Teruki Watabe, Satoshi Ito, Etsuro |
author_facet | Kyosei, Yuta Yamura, Sou Namba, Mayuri Yoshimura, Teruki Watabe, Satoshi Ito, Etsuro |
author_sort | Kyosei, Yuta |
collection | PubMed |
description | PCR diagnosis has been considered as the gold standard for coronavirus disease 2019 (COVID-19) and other many diseases. However, there are many problems in using PCR, such as non-specific (i.e., false-positive) and false-negative amplifications, the limits of a target sample volume, deactivation of the enzymes used, complicated techniques, difficulty in designing probe sequences, and the expense. We, thus, need an alternative to PCR, for example an ultrasensitive antigen test. In the present review, we summarize the following three topics. (1) The problems of PCR are outlined. (2) The antigen tests are surveyed in the literature that was published in 2020, and their pros and cons are discussed for commercially available antigen tests. (3) Our own antigen test on the basis of an ultrasensitive enzyme-linked immunosorbent assay (ELISA) is introduced. Finally, we discuss the possibility that our antigen test by an ultrasensitive ELISA technique will become the gold standard for diagnosis of COVID-19 and other diseases. |
format | Online Article Text |
id | pubmed-8049777 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | The Biophysical Society of Japan |
record_format | MEDLINE/PubMed |
spelling | pubmed-80497772021-05-04 Antigen tests for COVID-19 Kyosei, Yuta Yamura, Sou Namba, Mayuri Yoshimura, Teruki Watabe, Satoshi Ito, Etsuro Biophys Physicobiol Review Article (Invited) PCR diagnosis has been considered as the gold standard for coronavirus disease 2019 (COVID-19) and other many diseases. However, there are many problems in using PCR, such as non-specific (i.e., false-positive) and false-negative amplifications, the limits of a target sample volume, deactivation of the enzymes used, complicated techniques, difficulty in designing probe sequences, and the expense. We, thus, need an alternative to PCR, for example an ultrasensitive antigen test. In the present review, we summarize the following three topics. (1) The problems of PCR are outlined. (2) The antigen tests are surveyed in the literature that was published in 2020, and their pros and cons are discussed for commercially available antigen tests. (3) Our own antigen test on the basis of an ultrasensitive enzyme-linked immunosorbent assay (ELISA) is introduced. Finally, we discuss the possibility that our antigen test by an ultrasensitive ELISA technique will become the gold standard for diagnosis of COVID-19 and other diseases. The Biophysical Society of Japan 2021-02-10 /pmc/articles/PMC8049777/ /pubmed/33954080 http://dx.doi.org/10.2142/biophysico.bppb-v18.004 Text en 2021 THE BIOPHYSICAL SOCIETY OF JAPAN https://creativecommons.org/licenses/by-nc-sa/4.0/This article is licensed under the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. To view a copy of this license, visit
https://creativecommons.org/licenses/by-nc-sa/4.0/. |
spellingShingle | Review Article (Invited) Kyosei, Yuta Yamura, Sou Namba, Mayuri Yoshimura, Teruki Watabe, Satoshi Ito, Etsuro Antigen tests for COVID-19 |
title | Antigen tests for COVID-19 |
title_full | Antigen tests for COVID-19 |
title_fullStr | Antigen tests for COVID-19 |
title_full_unstemmed | Antigen tests for COVID-19 |
title_short | Antigen tests for COVID-19 |
title_sort | antigen tests for covid-19 |
topic | Review Article (Invited) |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8049777/ https://www.ncbi.nlm.nih.gov/pubmed/33954080 http://dx.doi.org/10.2142/biophysico.bppb-v18.004 |
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