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Deuteration Aiming for Neutron Scattering

The distinguished feature of neutron as a scattering probe is an isotope effect, especially the large difference in neutron scattering length between hydrogen and deuterium. The difference renders the different visibility between hydrogenated and deuterated proteins. Therefore, the combination of de...

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Detalles Bibliográficos
Autores principales: Okuda, Aya, Inoue, Rintaro, Morishima, Ken, Saio, Tomohide, Yunoki, Yasuhiro, Yagi-Utsumi, Maho, Yagi, Hirokazu, Shimizu, Masahiro, Sato, Nobuhiro, Urade, Reiko, Kato, Koichi, Sugiyama, Masaaki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Biophysical Society of Japan 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8049778/
https://www.ncbi.nlm.nih.gov/pubmed/33954079
http://dx.doi.org/10.2142/biophysico.bppb-v18.003
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author Okuda, Aya
Inoue, Rintaro
Morishima, Ken
Saio, Tomohide
Yunoki, Yasuhiro
Yagi-Utsumi, Maho
Yagi, Hirokazu
Shimizu, Masahiro
Sato, Nobuhiro
Urade, Reiko
Kato, Koichi
Sugiyama, Masaaki
author_facet Okuda, Aya
Inoue, Rintaro
Morishima, Ken
Saio, Tomohide
Yunoki, Yasuhiro
Yagi-Utsumi, Maho
Yagi, Hirokazu
Shimizu, Masahiro
Sato, Nobuhiro
Urade, Reiko
Kato, Koichi
Sugiyama, Masaaki
author_sort Okuda, Aya
collection PubMed
description The distinguished feature of neutron as a scattering probe is an isotope effect, especially the large difference in neutron scattering length between hydrogen and deuterium. The difference renders the different visibility between hydrogenated and deuterated proteins. Therefore, the combination of deuterated protein and neutron scattering enables the selective visualization of a target domain in the complex or a target protein in the multi-component system. Despite of this fascinating character, there exist several problems for the general use of this method: difficulty and high cost for protein deuteration, and control and determination of deuteration ratio of the sample. To resolve them, the protocol of protein deuteration techniques is presented in this report. It is strongly expected that this protocol will offer more opportunity for conducting the neutron scattering studies with deuterated proteins.
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spelling pubmed-80497782021-05-04 Deuteration Aiming for Neutron Scattering Okuda, Aya Inoue, Rintaro Morishima, Ken Saio, Tomohide Yunoki, Yasuhiro Yagi-Utsumi, Maho Yagi, Hirokazu Shimizu, Masahiro Sato, Nobuhiro Urade, Reiko Kato, Koichi Sugiyama, Masaaki Biophys Physicobiol Method and Protocol The distinguished feature of neutron as a scattering probe is an isotope effect, especially the large difference in neutron scattering length between hydrogen and deuterium. The difference renders the different visibility between hydrogenated and deuterated proteins. Therefore, the combination of deuterated protein and neutron scattering enables the selective visualization of a target domain in the complex or a target protein in the multi-component system. Despite of this fascinating character, there exist several problems for the general use of this method: difficulty and high cost for protein deuteration, and control and determination of deuteration ratio of the sample. To resolve them, the protocol of protein deuteration techniques is presented in this report. It is strongly expected that this protocol will offer more opportunity for conducting the neutron scattering studies with deuterated proteins. The Biophysical Society of Japan 2021-02-06 /pmc/articles/PMC8049778/ /pubmed/33954079 http://dx.doi.org/10.2142/biophysico.bppb-v18.003 Text en 2021 THE BIOPHYSICAL SOCIETY OF JAPAN https://creativecommons.org/licenses/by-nc-sa/4.0/This article is licensed under the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 Inter­national License. To view a copy of this license, visit 
https://creativecommons.org/licenses/by-nc-sa/4.0/.
spellingShingle Method and Protocol
Okuda, Aya
Inoue, Rintaro
Morishima, Ken
Saio, Tomohide
Yunoki, Yasuhiro
Yagi-Utsumi, Maho
Yagi, Hirokazu
Shimizu, Masahiro
Sato, Nobuhiro
Urade, Reiko
Kato, Koichi
Sugiyama, Masaaki
Deuteration Aiming for Neutron Scattering
title Deuteration Aiming for Neutron Scattering
title_full Deuteration Aiming for Neutron Scattering
title_fullStr Deuteration Aiming for Neutron Scattering
title_full_unstemmed Deuteration Aiming for Neutron Scattering
title_short Deuteration Aiming for Neutron Scattering
title_sort deuteration aiming for neutron scattering
topic Method and Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8049778/
https://www.ncbi.nlm.nih.gov/pubmed/33954079
http://dx.doi.org/10.2142/biophysico.bppb-v18.003
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