An in vitro tumorigenesis model based on live-cell-generated oxygen and nutrient gradients

The tumor microenvironment (TME) is multi-cellular, spatially heterogenous, and contains cell-generated gradients of soluble molecules. Current cell-based model systems lack this complexity or are difficult to interrogate microscopically. We present a 2D live-cell chamber that approximates the TME a...

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Autores principales: Gilmore, Anne C., Flaherty, Sarah J., Somasundaram, Veena, Scheiblin, David A., Lockett, Stephen J., Wink, David A., Heinz, William F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8050328/
https://www.ncbi.nlm.nih.gov/pubmed/33859337
http://dx.doi.org/10.1038/s42003-021-01954-0
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author Gilmore, Anne C.
Flaherty, Sarah J.
Somasundaram, Veena
Scheiblin, David A.
Lockett, Stephen J.
Wink, David A.
Heinz, William F.
author_facet Gilmore, Anne C.
Flaherty, Sarah J.
Somasundaram, Veena
Scheiblin, David A.
Lockett, Stephen J.
Wink, David A.
Heinz, William F.
author_sort Gilmore, Anne C.
collection PubMed
description The tumor microenvironment (TME) is multi-cellular, spatially heterogenous, and contains cell-generated gradients of soluble molecules. Current cell-based model systems lack this complexity or are difficult to interrogate microscopically. We present a 2D live-cell chamber that approximates the TME and demonstrate that breast cancer cells and macrophages generate hypoxic and nutrient gradients, self-organize, and have spatially varying phenotypes along the gradients, leading to new insights into tumorigenesis.
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spelling pubmed-80503282021-04-30 An in vitro tumorigenesis model based on live-cell-generated oxygen and nutrient gradients Gilmore, Anne C. Flaherty, Sarah J. Somasundaram, Veena Scheiblin, David A. Lockett, Stephen J. Wink, David A. Heinz, William F. Commun Biol Article The tumor microenvironment (TME) is multi-cellular, spatially heterogenous, and contains cell-generated gradients of soluble molecules. Current cell-based model systems lack this complexity or are difficult to interrogate microscopically. We present a 2D live-cell chamber that approximates the TME and demonstrate that breast cancer cells and macrophages generate hypoxic and nutrient gradients, self-organize, and have spatially varying phenotypes along the gradients, leading to new insights into tumorigenesis. Nature Publishing Group UK 2021-04-15 /pmc/articles/PMC8050328/ /pubmed/33859337 http://dx.doi.org/10.1038/s42003-021-01954-0 Text en © This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Gilmore, Anne C.
Flaherty, Sarah J.
Somasundaram, Veena
Scheiblin, David A.
Lockett, Stephen J.
Wink, David A.
Heinz, William F.
An in vitro tumorigenesis model based on live-cell-generated oxygen and nutrient gradients
title An in vitro tumorigenesis model based on live-cell-generated oxygen and nutrient gradients
title_full An in vitro tumorigenesis model based on live-cell-generated oxygen and nutrient gradients
title_fullStr An in vitro tumorigenesis model based on live-cell-generated oxygen and nutrient gradients
title_full_unstemmed An in vitro tumorigenesis model based on live-cell-generated oxygen and nutrient gradients
title_short An in vitro tumorigenesis model based on live-cell-generated oxygen and nutrient gradients
title_sort in vitro tumorigenesis model based on live-cell-generated oxygen and nutrient gradients
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8050328/
https://www.ncbi.nlm.nih.gov/pubmed/33859337
http://dx.doi.org/10.1038/s42003-021-01954-0
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