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Telomere length assessment in blood leukocytes of patients with sarcoidosis

BACKGROUND: Accelerated aging and telomere shortening have been studied in many chronic diseases such as interstitial pulmonary fibrosis and chronic obstructive pulmonary disease. Different studies have shown that patients with these diseases have shorter telomere lengths than controls; this can be...

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Autores principales: Afshar, Hale, Abedini, Atefeh, Nadji, Seyed Alireza, Sadr, Makan, Kiani, Arda, Alizadeh, Niloufar, Javadi, Alireza
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Mattioli 1885 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8050626/
https://www.ncbi.nlm.nih.gov/pubmed/33867793
http://dx.doi.org/10.36141/svdld.v38i1.10705
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author Afshar, Hale
Abedini, Atefeh
Nadji, Seyed Alireza
Sadr, Makan
Kiani, Arda
Alizadeh, Niloufar
Javadi, Alireza
author_facet Afshar, Hale
Abedini, Atefeh
Nadji, Seyed Alireza
Sadr, Makan
Kiani, Arda
Alizadeh, Niloufar
Javadi, Alireza
author_sort Afshar, Hale
collection PubMed
description BACKGROUND: Accelerated aging and telomere shortening have been studied in many chronic diseases such as interstitial pulmonary fibrosis and chronic obstructive pulmonary disease. Different studies have shown that patients with these diseases have shorter telomere lengths than controls; this can be a marker of the progression and outcome of the disease. So far, a few studies have been evaluated the telomere length in sarcoidosis. In this study we determine the telomere length in patients with sarcoidosis and compare it with control subjects. OBJECTIVE: Our aim is to compare telomere length in patients with sarcoidosis and normal population. Methods: We select 58 patients with sarcoidosis who were visited in the sarcoidosis clinic of Masih Daneshvari Hospital. 58 sex and age-matched (with±2 years) healthy control subjects were selected. Telomere length was measured by quantitative real time PCR as described by Cawthon on peripheral blood sample. The telomere repeat copy number (T) to single-gene copy number(S) ratio was calculated using the comparative Ct method. Results: The mean and standard deviation of telomere length in the patient and control group was 0.65 ± 0.05 and 0.72 ± 0.07 respectively. There was a statistically significant difference between the two groups. (P = 0.031). Conclusion: Sarcoidosis is an inflammatory disease that can involve many organs. Like other chronic diseases, aging phenomenon occurs in that; which led to decrease cellular and tissue telomere length. This article demonstrates shorter telomere length in Iranian sarcoidosis patients compared to normal population.
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spelling pubmed-80506262021-04-16 Telomere length assessment in blood leukocytes of patients with sarcoidosis Afshar, Hale Abedini, Atefeh Nadji, Seyed Alireza Sadr, Makan Kiani, Arda Alizadeh, Niloufar Javadi, Alireza Sarcoidosis Vasc Diffuse Lung Dis Original Article: Clinical Research BACKGROUND: Accelerated aging and telomere shortening have been studied in many chronic diseases such as interstitial pulmonary fibrosis and chronic obstructive pulmonary disease. Different studies have shown that patients with these diseases have shorter telomere lengths than controls; this can be a marker of the progression and outcome of the disease. So far, a few studies have been evaluated the telomere length in sarcoidosis. In this study we determine the telomere length in patients with sarcoidosis and compare it with control subjects. OBJECTIVE: Our aim is to compare telomere length in patients with sarcoidosis and normal population. Methods: We select 58 patients with sarcoidosis who were visited in the sarcoidosis clinic of Masih Daneshvari Hospital. 58 sex and age-matched (with±2 years) healthy control subjects were selected. Telomere length was measured by quantitative real time PCR as described by Cawthon on peripheral blood sample. The telomere repeat copy number (T) to single-gene copy number(S) ratio was calculated using the comparative Ct method. Results: The mean and standard deviation of telomere length in the patient and control group was 0.65 ± 0.05 and 0.72 ± 0.07 respectively. There was a statistically significant difference between the two groups. (P = 0.031). Conclusion: Sarcoidosis is an inflammatory disease that can involve many organs. Like other chronic diseases, aging phenomenon occurs in that; which led to decrease cellular and tissue telomere length. This article demonstrates shorter telomere length in Iranian sarcoidosis patients compared to normal population. Mattioli 1885 2021 2021-03-31 /pmc/articles/PMC8050626/ /pubmed/33867793 http://dx.doi.org/10.36141/svdld.v38i1.10705 Text en Copyright: © 2020 SARCOIDOSIS VASCULITIS AND DIFFUSE LUNG DISEASES https://creativecommons.org/licenses/by-nc-sa/4.0/This work is licensed under a Creative Commons Attribution 4.0 International License
spellingShingle Original Article: Clinical Research
Afshar, Hale
Abedini, Atefeh
Nadji, Seyed Alireza
Sadr, Makan
Kiani, Arda
Alizadeh, Niloufar
Javadi, Alireza
Telomere length assessment in blood leukocytes of patients with sarcoidosis
title Telomere length assessment in blood leukocytes of patients with sarcoidosis
title_full Telomere length assessment in blood leukocytes of patients with sarcoidosis
title_fullStr Telomere length assessment in blood leukocytes of patients with sarcoidosis
title_full_unstemmed Telomere length assessment in blood leukocytes of patients with sarcoidosis
title_short Telomere length assessment in blood leukocytes of patients with sarcoidosis
title_sort telomere length assessment in blood leukocytes of patients with sarcoidosis
topic Original Article: Clinical Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8050626/
https://www.ncbi.nlm.nih.gov/pubmed/33867793
http://dx.doi.org/10.36141/svdld.v38i1.10705
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