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Alkaline phosphatase downregulation promotes lung adenocarcinoma metastasis via the c-Myc/RhoA axis

BACKGROUND: Lung adenocarcinoma (LUAD) metastasis significantly reduces patient survival; hence inhibiting the metastatic ability of lung cancer cells will greatly prolong patient survival. Alkaline phosphatase (ALPL), a homodimeric cell surface phosphohydrolase, is reported to play a controversial...

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Autores principales: Lou, Zhefeng, Lin, Weiwei, Zhao, Huirong, Jiao, Xueli, Wang, Cong, Zhao, He, Liu, Lu, Liu, Yu, Xie, Qipeng, Huang, Xing, Huang, Haishan, Zhao, Lingling
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8050923/
https://www.ncbi.nlm.nih.gov/pubmed/33858415
http://dx.doi.org/10.1186/s12935-021-01919-7
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author Lou, Zhefeng
Lin, Weiwei
Zhao, Huirong
Jiao, Xueli
Wang, Cong
Zhao, He
Liu, Lu
Liu, Yu
Xie, Qipeng
Huang, Xing
Huang, Haishan
Zhao, Lingling
author_facet Lou, Zhefeng
Lin, Weiwei
Zhao, Huirong
Jiao, Xueli
Wang, Cong
Zhao, He
Liu, Lu
Liu, Yu
Xie, Qipeng
Huang, Xing
Huang, Haishan
Zhao, Lingling
author_sort Lou, Zhefeng
collection PubMed
description BACKGROUND: Lung adenocarcinoma (LUAD) metastasis significantly reduces patient survival; hence inhibiting the metastatic ability of lung cancer cells will greatly prolong patient survival. Alkaline phosphatase (ALPL), a homodimeric cell surface phosphohydrolase, is reported to play a controversial role in prostate cancer and ovarian cancer cell migration; however, the function of ALPL in LUAD and the related mechanisms remain unclear. METHODS: TCGA database was used to analysis the expression of ALPL, and further verification was performed in a cohort of 36 LUAD samples by qPCR and western blot. Soft-agar assay, transwell assay and lung metastasis assay were employed to detect the function of ALPL in LUAD progression. The qPCR, luciferase promoter reporter assay and western blot were used to clarify the molecular mechanisms of ALPL in promoting metastasis in LUAD. RESULTS: ALPL was downregulated in LUAD, and the disease-free survival rate of patients with low ALPL was significantly reduced. Further studies showed that overexpression of ALPL in LUAD cell lines did not significantly affect cell proliferation, but it did significantly attenuate lung metastasis in a mouse model. ALPL downregulation in LUAD led to a decrease in the amount of phosphorylated (p)-ERK. Because p-ERK promotes the classical c-Myc degradation pathway, the decrease in p-ERK led to the accumulation of c-Myc and therefore to an increase in RhoA transcription, which enhanced LUAD cell metastasis. CONCLUSION: ALPL specially inhibits the metastasis of LUAD cells by affecting the p-ERK/c-Myc/RhoA axis, providing a theoretical basis for the targeted therapy of clinical LUAD. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12935-021-01919-7.
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spelling pubmed-80509232021-04-19 Alkaline phosphatase downregulation promotes lung adenocarcinoma metastasis via the c-Myc/RhoA axis Lou, Zhefeng Lin, Weiwei Zhao, Huirong Jiao, Xueli Wang, Cong Zhao, He Liu, Lu Liu, Yu Xie, Qipeng Huang, Xing Huang, Haishan Zhao, Lingling Cancer Cell Int Primary Research BACKGROUND: Lung adenocarcinoma (LUAD) metastasis significantly reduces patient survival; hence inhibiting the metastatic ability of lung cancer cells will greatly prolong patient survival. Alkaline phosphatase (ALPL), a homodimeric cell surface phosphohydrolase, is reported to play a controversial role in prostate cancer and ovarian cancer cell migration; however, the function of ALPL in LUAD and the related mechanisms remain unclear. METHODS: TCGA database was used to analysis the expression of ALPL, and further verification was performed in a cohort of 36 LUAD samples by qPCR and western blot. Soft-agar assay, transwell assay and lung metastasis assay were employed to detect the function of ALPL in LUAD progression. The qPCR, luciferase promoter reporter assay and western blot were used to clarify the molecular mechanisms of ALPL in promoting metastasis in LUAD. RESULTS: ALPL was downregulated in LUAD, and the disease-free survival rate of patients with low ALPL was significantly reduced. Further studies showed that overexpression of ALPL in LUAD cell lines did not significantly affect cell proliferation, but it did significantly attenuate lung metastasis in a mouse model. ALPL downregulation in LUAD led to a decrease in the amount of phosphorylated (p)-ERK. Because p-ERK promotes the classical c-Myc degradation pathway, the decrease in p-ERK led to the accumulation of c-Myc and therefore to an increase in RhoA transcription, which enhanced LUAD cell metastasis. CONCLUSION: ALPL specially inhibits the metastasis of LUAD cells by affecting the p-ERK/c-Myc/RhoA axis, providing a theoretical basis for the targeted therapy of clinical LUAD. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12935-021-01919-7. BioMed Central 2021-04-15 /pmc/articles/PMC8050923/ /pubmed/33858415 http://dx.doi.org/10.1186/s12935-021-01919-7 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Primary Research
Lou, Zhefeng
Lin, Weiwei
Zhao, Huirong
Jiao, Xueli
Wang, Cong
Zhao, He
Liu, Lu
Liu, Yu
Xie, Qipeng
Huang, Xing
Huang, Haishan
Zhao, Lingling
Alkaline phosphatase downregulation promotes lung adenocarcinoma metastasis via the c-Myc/RhoA axis
title Alkaline phosphatase downregulation promotes lung adenocarcinoma metastasis via the c-Myc/RhoA axis
title_full Alkaline phosphatase downregulation promotes lung adenocarcinoma metastasis via the c-Myc/RhoA axis
title_fullStr Alkaline phosphatase downregulation promotes lung adenocarcinoma metastasis via the c-Myc/RhoA axis
title_full_unstemmed Alkaline phosphatase downregulation promotes lung adenocarcinoma metastasis via the c-Myc/RhoA axis
title_short Alkaline phosphatase downregulation promotes lung adenocarcinoma metastasis via the c-Myc/RhoA axis
title_sort alkaline phosphatase downregulation promotes lung adenocarcinoma metastasis via the c-myc/rhoa axis
topic Primary Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8050923/
https://www.ncbi.nlm.nih.gov/pubmed/33858415
http://dx.doi.org/10.1186/s12935-021-01919-7
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