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Prevalence and genotype distribution of human papillomavirus in Czech non-vaccinated heterosexual couples

BACKGROUND: Data about the genotype-specific human papillomavirus (HPV) prevalence in the Czech Republic is limited. We aimed to evaluate the prevalence and concordance of genotype-specific HPV infection detected in semen samples, penile swabs and cervical swabs from non-vaccinated heterosexual coup...

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Autores principales: Jaworek, Hana, Koudelakova, Vladimira, Oborna, Ivana, Zborilova, Blazena, Brezinova, Jana, Ruzickova, Dagmar, Vrbkova, Jana, Kourilova, Pavla, Hajduch, Marian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8051085/
https://www.ncbi.nlm.nih.gov/pubmed/33858457
http://dx.doi.org/10.1186/s12985-021-01551-x
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author Jaworek, Hana
Koudelakova, Vladimira
Oborna, Ivana
Zborilova, Blazena
Brezinova, Jana
Ruzickova, Dagmar
Vrbkova, Jana
Kourilova, Pavla
Hajduch, Marian
author_facet Jaworek, Hana
Koudelakova, Vladimira
Oborna, Ivana
Zborilova, Blazena
Brezinova, Jana
Ruzickova, Dagmar
Vrbkova, Jana
Kourilova, Pavla
Hajduch, Marian
author_sort Jaworek, Hana
collection PubMed
description BACKGROUND: Data about the genotype-specific human papillomavirus (HPV) prevalence in the Czech Republic is limited. We aimed to evaluate the prevalence and concordance of genotype-specific HPV infection detected in semen samples, penile swabs and cervical swabs from non-vaccinated heterosexual couples without HPV-associated disease. METHODS: Semen samples and penile swabs were collected from male partners and cervical swabs were collected from female partners of heterosexual couples treated for infertility (n = 195). Presence of HPV DNA in semen samples and cervical swabs was analyzed using the cobas(®) HPV Test and PapilloCheck(®). Only the PapilloCheck(®) test was used to detect HPV in penile swabs. The genotype-specific prevalence and concordance of HPV infection not targeted by vaccine were evaluated using Fisher exact test. RESULTS: Both partners were infected with any HPV type in 13.8% (27/195) of couples and, of these couples, 55.6% (15/27) harbored at least one mutual genotype. High-risk HPV (hrHPV) genotypes were detected in 12.3% (24/195) of semen samples, 31.3% (61/195) of penile swabs, and 19.5% (38/195) of cervical swabs (P < 0.001). The most prevalent hrHPV genotype were HPV53 (2.56%; 5/195) in semen samples, HPV16 (6.67%, 13/195) in penile swabs and HPV39 (3.59%, 7/195) in cervical swabs. Low-risk (lrHPV) genotypes were detected in 5.13% (10/195) of semen samples, 15.9% (31/195) of penile swabs, and 4.10% (8/195) of cervical swabs (P < 0.001). Male sexual partners of HPV-positive women were more likely to be infected with at least one of the same HPV types than female sexual partners of HPV-positive men (34.9% vs. 17.9%, P = 0.055). CONCLUSIONS: This study showed that the detection of HPV infection differ by anatomic site and gender. Regardless the anatomic site, high prevalence of HPV genital infection was found in both Czech men and women. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12985-021-01551-x.
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spelling pubmed-80510852021-04-19 Prevalence and genotype distribution of human papillomavirus in Czech non-vaccinated heterosexual couples Jaworek, Hana Koudelakova, Vladimira Oborna, Ivana Zborilova, Blazena Brezinova, Jana Ruzickova, Dagmar Vrbkova, Jana Kourilova, Pavla Hajduch, Marian Virol J Research BACKGROUND: Data about the genotype-specific human papillomavirus (HPV) prevalence in the Czech Republic is limited. We aimed to evaluate the prevalence and concordance of genotype-specific HPV infection detected in semen samples, penile swabs and cervical swabs from non-vaccinated heterosexual couples without HPV-associated disease. METHODS: Semen samples and penile swabs were collected from male partners and cervical swabs were collected from female partners of heterosexual couples treated for infertility (n = 195). Presence of HPV DNA in semen samples and cervical swabs was analyzed using the cobas(®) HPV Test and PapilloCheck(®). Only the PapilloCheck(®) test was used to detect HPV in penile swabs. The genotype-specific prevalence and concordance of HPV infection not targeted by vaccine were evaluated using Fisher exact test. RESULTS: Both partners were infected with any HPV type in 13.8% (27/195) of couples and, of these couples, 55.6% (15/27) harbored at least one mutual genotype. High-risk HPV (hrHPV) genotypes were detected in 12.3% (24/195) of semen samples, 31.3% (61/195) of penile swabs, and 19.5% (38/195) of cervical swabs (P < 0.001). The most prevalent hrHPV genotype were HPV53 (2.56%; 5/195) in semen samples, HPV16 (6.67%, 13/195) in penile swabs and HPV39 (3.59%, 7/195) in cervical swabs. Low-risk (lrHPV) genotypes were detected in 5.13% (10/195) of semen samples, 15.9% (31/195) of penile swabs, and 4.10% (8/195) of cervical swabs (P < 0.001). Male sexual partners of HPV-positive women were more likely to be infected with at least one of the same HPV types than female sexual partners of HPV-positive men (34.9% vs. 17.9%, P = 0.055). CONCLUSIONS: This study showed that the detection of HPV infection differ by anatomic site and gender. Regardless the anatomic site, high prevalence of HPV genital infection was found in both Czech men and women. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12985-021-01551-x. BioMed Central 2021-04-15 /pmc/articles/PMC8051085/ /pubmed/33858457 http://dx.doi.org/10.1186/s12985-021-01551-x Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Jaworek, Hana
Koudelakova, Vladimira
Oborna, Ivana
Zborilova, Blazena
Brezinova, Jana
Ruzickova, Dagmar
Vrbkova, Jana
Kourilova, Pavla
Hajduch, Marian
Prevalence and genotype distribution of human papillomavirus in Czech non-vaccinated heterosexual couples
title Prevalence and genotype distribution of human papillomavirus in Czech non-vaccinated heterosexual couples
title_full Prevalence and genotype distribution of human papillomavirus in Czech non-vaccinated heterosexual couples
title_fullStr Prevalence and genotype distribution of human papillomavirus in Czech non-vaccinated heterosexual couples
title_full_unstemmed Prevalence and genotype distribution of human papillomavirus in Czech non-vaccinated heterosexual couples
title_short Prevalence and genotype distribution of human papillomavirus in Czech non-vaccinated heterosexual couples
title_sort prevalence and genotype distribution of human papillomavirus in czech non-vaccinated heterosexual couples
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8051085/
https://www.ncbi.nlm.nih.gov/pubmed/33858457
http://dx.doi.org/10.1186/s12985-021-01551-x
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