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Overexpression of GmMYB14 improves high‐density yield and drought tolerance of soybean through regulating plant architecture mediated by the brassinosteroid pathway

MYB transcription factors (TFs) have been reported to regulate the biosynthesis of secondary metabolites, as well as to mediate plant adaption to abiotic stresses, including drought. However, the roles of MYB TFs in regulating plant architecture and yield potential remain poorly understood. Here, we...

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Autores principales: Chen, Limiao, Yang, Hongli, Fang, Yisheng, Guo, Wei, Chen, Haifeng, Zhang, Xiaojuan, Dai, Wenjun, Chen, Shuilian, Hao, Qingnan, Yuan, Songli, Zhang, Chanjuan, Huang, Yi, Shan, Zhihui, Yang, Zhonglu, Qiu, Dezhen, Liu, Xiaorong, Tran, Lam‐Son Phan, Zhou, Xinan, Cao, Dong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8051608/
https://www.ncbi.nlm.nih.gov/pubmed/33098207
http://dx.doi.org/10.1111/pbi.13496
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author Chen, Limiao
Yang, Hongli
Fang, Yisheng
Guo, Wei
Chen, Haifeng
Zhang, Xiaojuan
Dai, Wenjun
Chen, Shuilian
Hao, Qingnan
Yuan, Songli
Zhang, Chanjuan
Huang, Yi
Shan, Zhihui
Yang, Zhonglu
Qiu, Dezhen
Liu, Xiaorong
Tran, Lam‐Son Phan
Zhou, Xinan
Cao, Dong
author_facet Chen, Limiao
Yang, Hongli
Fang, Yisheng
Guo, Wei
Chen, Haifeng
Zhang, Xiaojuan
Dai, Wenjun
Chen, Shuilian
Hao, Qingnan
Yuan, Songli
Zhang, Chanjuan
Huang, Yi
Shan, Zhihui
Yang, Zhonglu
Qiu, Dezhen
Liu, Xiaorong
Tran, Lam‐Son Phan
Zhou, Xinan
Cao, Dong
author_sort Chen, Limiao
collection PubMed
description MYB transcription factors (TFs) have been reported to regulate the biosynthesis of secondary metabolites, as well as to mediate plant adaption to abiotic stresses, including drought. However, the roles of MYB TFs in regulating plant architecture and yield potential remain poorly understood. Here, we studied the roles of the dehydration‐inducible GmMYB14 gene in regulating plant architecture, high‐density yield and drought tolerance through the brassinosteroid (BR) pathway in soybean. GmMYB14 was shown to localize to nucleus and has a transactivation activity. Stable GmMYB14‐overexpressing (GmMYB14‐OX) transgenic soybean plants displayed a semi‐dwarfism and compact plant architecture associated with decreased cell size, resulting in a decrease in plant height, internode length, leaf area, leaf petiole length and leaf petiole angle, and improved yield in high density under field conditions. Results of the transcriptome sequencing suggested the involvement of BRs in regulating GmMYB14‐OX plant architecture. Indeed, GmMYB14‐OX plants showed reduced endogenous BR contents, while exogenous application of brassinolide could partly rescue the phenotype of GmMYB14‐OX plants. Furthermore, GmMYB14 was shown to directly bind to the promoter of GmBEN1 and up‐regulate its expression, leading to reduced BR content in GmMYB14‐OX plants. GmMYB14‐OX plants also displayed improved drought tolerance under field conditions. GmBEN1 expression was also up‐regulated in the leaves of GmMYB14‐OX plants under polyethylene glycol treatment, indicating that the GmBEN1‐mediated reduction in BR level under stress also contributed to drought/osmotic stress tolerance of the transgenic plants. Our findings provided a strategy for stably increasing high‐density yield and drought tolerance in soybean using a single TF‐encoding gene.
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spelling pubmed-80516082021-04-21 Overexpression of GmMYB14 improves high‐density yield and drought tolerance of soybean through regulating plant architecture mediated by the brassinosteroid pathway Chen, Limiao Yang, Hongli Fang, Yisheng Guo, Wei Chen, Haifeng Zhang, Xiaojuan Dai, Wenjun Chen, Shuilian Hao, Qingnan Yuan, Songli Zhang, Chanjuan Huang, Yi Shan, Zhihui Yang, Zhonglu Qiu, Dezhen Liu, Xiaorong Tran, Lam‐Son Phan Zhou, Xinan Cao, Dong Plant Biotechnol J Research Articles MYB transcription factors (TFs) have been reported to regulate the biosynthesis of secondary metabolites, as well as to mediate plant adaption to abiotic stresses, including drought. However, the roles of MYB TFs in regulating plant architecture and yield potential remain poorly understood. Here, we studied the roles of the dehydration‐inducible GmMYB14 gene in regulating plant architecture, high‐density yield and drought tolerance through the brassinosteroid (BR) pathway in soybean. GmMYB14 was shown to localize to nucleus and has a transactivation activity. Stable GmMYB14‐overexpressing (GmMYB14‐OX) transgenic soybean plants displayed a semi‐dwarfism and compact plant architecture associated with decreased cell size, resulting in a decrease in plant height, internode length, leaf area, leaf petiole length and leaf petiole angle, and improved yield in high density under field conditions. Results of the transcriptome sequencing suggested the involvement of BRs in regulating GmMYB14‐OX plant architecture. Indeed, GmMYB14‐OX plants showed reduced endogenous BR contents, while exogenous application of brassinolide could partly rescue the phenotype of GmMYB14‐OX plants. Furthermore, GmMYB14 was shown to directly bind to the promoter of GmBEN1 and up‐regulate its expression, leading to reduced BR content in GmMYB14‐OX plants. GmMYB14‐OX plants also displayed improved drought tolerance under field conditions. GmBEN1 expression was also up‐regulated in the leaves of GmMYB14‐OX plants under polyethylene glycol treatment, indicating that the GmBEN1‐mediated reduction in BR level under stress also contributed to drought/osmotic stress tolerance of the transgenic plants. Our findings provided a strategy for stably increasing high‐density yield and drought tolerance in soybean using a single TF‐encoding gene. John Wiley and Sons Inc. 2020-11-23 2021-04 /pmc/articles/PMC8051608/ /pubmed/33098207 http://dx.doi.org/10.1111/pbi.13496 Text en © 2020 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Chen, Limiao
Yang, Hongli
Fang, Yisheng
Guo, Wei
Chen, Haifeng
Zhang, Xiaojuan
Dai, Wenjun
Chen, Shuilian
Hao, Qingnan
Yuan, Songli
Zhang, Chanjuan
Huang, Yi
Shan, Zhihui
Yang, Zhonglu
Qiu, Dezhen
Liu, Xiaorong
Tran, Lam‐Son Phan
Zhou, Xinan
Cao, Dong
Overexpression of GmMYB14 improves high‐density yield and drought tolerance of soybean through regulating plant architecture mediated by the brassinosteroid pathway
title Overexpression of GmMYB14 improves high‐density yield and drought tolerance of soybean through regulating plant architecture mediated by the brassinosteroid pathway
title_full Overexpression of GmMYB14 improves high‐density yield and drought tolerance of soybean through regulating plant architecture mediated by the brassinosteroid pathway
title_fullStr Overexpression of GmMYB14 improves high‐density yield and drought tolerance of soybean through regulating plant architecture mediated by the brassinosteroid pathway
title_full_unstemmed Overexpression of GmMYB14 improves high‐density yield and drought tolerance of soybean through regulating plant architecture mediated by the brassinosteroid pathway
title_short Overexpression of GmMYB14 improves high‐density yield and drought tolerance of soybean through regulating plant architecture mediated by the brassinosteroid pathway
title_sort overexpression of gmmyb14 improves high‐density yield and drought tolerance of soybean through regulating plant architecture mediated by the brassinosteroid pathway
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8051608/
https://www.ncbi.nlm.nih.gov/pubmed/33098207
http://dx.doi.org/10.1111/pbi.13496
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