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Overexpression of alfalfa SIMK promotes root hair growth, nodule clustering and shoot biomass production
Nitrogen‐fixing rhizobia and legumes have developed complex mutualistic mechanism that allows to convert atmospheric nitrogen into ammonia. Signalling by mitogen‐activated protein kinases (MAPKs) seems to be involved in this symbiotic interaction. Previously, we reported that stress‐induced MAPK (SI...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8051612/ https://www.ncbi.nlm.nih.gov/pubmed/33112469 http://dx.doi.org/10.1111/pbi.13503 |
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author | Hrbáčková, Miroslava Luptovčiak, Ivan Hlaváčková, Kateřina Dvořák, Petr Tichá, Michaela Šamajová, Olga Novák, Dominik Bednarz, Hanna Niehaus, Karsten Ovečka, Miroslav Šamaj, Jozef |
author_facet | Hrbáčková, Miroslava Luptovčiak, Ivan Hlaváčková, Kateřina Dvořák, Petr Tichá, Michaela Šamajová, Olga Novák, Dominik Bednarz, Hanna Niehaus, Karsten Ovečka, Miroslav Šamaj, Jozef |
author_sort | Hrbáčková, Miroslava |
collection | PubMed |
description | Nitrogen‐fixing rhizobia and legumes have developed complex mutualistic mechanism that allows to convert atmospheric nitrogen into ammonia. Signalling by mitogen‐activated protein kinases (MAPKs) seems to be involved in this symbiotic interaction. Previously, we reported that stress‐induced MAPK (SIMK) shows predominantly nuclear localization in alfalfa root epidermal cells. Nevertheless, SIMK is activated and relocalized to the tips of growing root hairs during their development. SIMK kinase (SIMKK) is a well‐known upstream activator of SIMK. Here, we characterized production parameters of transgenic alfalfa plants with genetically manipulated SIMK after infection with Sinorhizobium meliloti. SIMKK RNAi lines, causing strong downregulation of both SIMKK and SIMK, showed reduced root hair growth and lower capacity to form infection threads and nodules. In contrast, constitutive overexpression of GFP‐tagged SIMK promoted root hair growth as well as infection thread and nodule clustering. Moreover, SIMKK and SIMK downregulation led to decrease, while overexpression of GFP‐tagged SIMK led to increase of biomass in above‐ground part of plants. These data suggest that genetic manipulations causing downregulation or overexpression of SIMK affect root hair, nodule and shoot formation patterns in alfalfa, and point to the new biotechnological potential of this MAPK. |
format | Online Article Text |
id | pubmed-8051612 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-80516122021-04-21 Overexpression of alfalfa SIMK promotes root hair growth, nodule clustering and shoot biomass production Hrbáčková, Miroslava Luptovčiak, Ivan Hlaváčková, Kateřina Dvořák, Petr Tichá, Michaela Šamajová, Olga Novák, Dominik Bednarz, Hanna Niehaus, Karsten Ovečka, Miroslav Šamaj, Jozef Plant Biotechnol J Research Articles Nitrogen‐fixing rhizobia and legumes have developed complex mutualistic mechanism that allows to convert atmospheric nitrogen into ammonia. Signalling by mitogen‐activated protein kinases (MAPKs) seems to be involved in this symbiotic interaction. Previously, we reported that stress‐induced MAPK (SIMK) shows predominantly nuclear localization in alfalfa root epidermal cells. Nevertheless, SIMK is activated and relocalized to the tips of growing root hairs during their development. SIMK kinase (SIMKK) is a well‐known upstream activator of SIMK. Here, we characterized production parameters of transgenic alfalfa plants with genetically manipulated SIMK after infection with Sinorhizobium meliloti. SIMKK RNAi lines, causing strong downregulation of both SIMKK and SIMK, showed reduced root hair growth and lower capacity to form infection threads and nodules. In contrast, constitutive overexpression of GFP‐tagged SIMK promoted root hair growth as well as infection thread and nodule clustering. Moreover, SIMKK and SIMK downregulation led to decrease, while overexpression of GFP‐tagged SIMK led to increase of biomass in above‐ground part of plants. These data suggest that genetic manipulations causing downregulation or overexpression of SIMK affect root hair, nodule and shoot formation patterns in alfalfa, and point to the new biotechnological potential of this MAPK. John Wiley and Sons Inc. 2020-11-28 2021-04 /pmc/articles/PMC8051612/ /pubmed/33112469 http://dx.doi.org/10.1111/pbi.13503 Text en © 2020 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Research Articles Hrbáčková, Miroslava Luptovčiak, Ivan Hlaváčková, Kateřina Dvořák, Petr Tichá, Michaela Šamajová, Olga Novák, Dominik Bednarz, Hanna Niehaus, Karsten Ovečka, Miroslav Šamaj, Jozef Overexpression of alfalfa SIMK promotes root hair growth, nodule clustering and shoot biomass production |
title | Overexpression of alfalfa SIMK promotes root hair growth, nodule clustering and shoot biomass production |
title_full | Overexpression of alfalfa SIMK promotes root hair growth, nodule clustering and shoot biomass production |
title_fullStr | Overexpression of alfalfa SIMK promotes root hair growth, nodule clustering and shoot biomass production |
title_full_unstemmed | Overexpression of alfalfa SIMK promotes root hair growth, nodule clustering and shoot biomass production |
title_short | Overexpression of alfalfa SIMK promotes root hair growth, nodule clustering and shoot biomass production |
title_sort | overexpression of alfalfa simk promotes root hair growth, nodule clustering and shoot biomass production |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8051612/ https://www.ncbi.nlm.nih.gov/pubmed/33112469 http://dx.doi.org/10.1111/pbi.13503 |
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