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Comparison of calretinin expression in dentigerous cysts and ameloblastoma: An immunohistochemical study

BACKGROUND: Calretinin is a calcium-binding protein of 29-kilodalton (kDa), which is widely expressed in normal human tissues and tumorigenic tissues. Its expression in the odontogenic epithelium during odontogenesis and in neoplastic odontogenic tissues has been demonstrated. Unicystic ameloblastom...

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Autores principales: Varshney, Anchal, Aggarwal, Shivani, Gill, Sharanjeet Kaur, Aggarwal, Ashim, Jaiswal, Yashmi, Sharma, Jaishree
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wolters Kluwer - Medknow 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8051659/
https://www.ncbi.nlm.nih.gov/pubmed/33897185
http://dx.doi.org/10.4103/njms.NJMS_71_19
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author Varshney, Anchal
Aggarwal, Shivani
Gill, Sharanjeet Kaur
Aggarwal, Ashim
Jaiswal, Yashmi
Sharma, Jaishree
author_facet Varshney, Anchal
Aggarwal, Shivani
Gill, Sharanjeet Kaur
Aggarwal, Ashim
Jaiswal, Yashmi
Sharma, Jaishree
author_sort Varshney, Anchal
collection PubMed
description BACKGROUND: Calretinin is a calcium-binding protein of 29-kilodalton (kDa), which is widely expressed in normal human tissues and tumorigenic tissues. Its expression in the odontogenic epithelium during odontogenesis and in neoplastic odontogenic tissues has been demonstrated. Unicystic ameloblastoma poses a diagnostic challenge, as its histologic presentation can be sometimes mistaken for cystic odontogenic lesions. In the present study, an attempt is made to overcome the confusion encountered in the diagnosis of dentigerous cyst and unicystic ameloblastoma, using the expression of calretinin in both lesions and to compare this expression with conventional ameloblastoma to accurately diagnose and differentiate these lesions. MATERIALS AND METHODS: A total of eighty cases, in which twenty cases each of ameloblastoma, unicystic ameloblastoma, dentigerous cyst, and odontogenic keratocyst (OKC) were included in the study. Slides were made from the archival blocks of each case and were stained immunohistochemically with calretinin. RESULTS: Correlation between calretinin staining and histopathological diagnosis was done, and it was found that all twenty cases of ameloblastoma showed positivity for calretinin, whereas 17 of twenty cases of unicystic ameloblastoma showed positivity for calretinin staining. All the cases of OKC and dentigerous cyst were negative for calretinin. CONCLUSION: Calretinin may serve as an important diagnostic adjunct in the differential diagnosis of ameloblastoma and cystic odontogenic lesions.
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spelling pubmed-80516592021-04-23 Comparison of calretinin expression in dentigerous cysts and ameloblastoma: An immunohistochemical study Varshney, Anchal Aggarwal, Shivani Gill, Sharanjeet Kaur Aggarwal, Ashim Jaiswal, Yashmi Sharma, Jaishree Natl J Maxillofac Surg Original Article BACKGROUND: Calretinin is a calcium-binding protein of 29-kilodalton (kDa), which is widely expressed in normal human tissues and tumorigenic tissues. Its expression in the odontogenic epithelium during odontogenesis and in neoplastic odontogenic tissues has been demonstrated. Unicystic ameloblastoma poses a diagnostic challenge, as its histologic presentation can be sometimes mistaken for cystic odontogenic lesions. In the present study, an attempt is made to overcome the confusion encountered in the diagnosis of dentigerous cyst and unicystic ameloblastoma, using the expression of calretinin in both lesions and to compare this expression with conventional ameloblastoma to accurately diagnose and differentiate these lesions. MATERIALS AND METHODS: A total of eighty cases, in which twenty cases each of ameloblastoma, unicystic ameloblastoma, dentigerous cyst, and odontogenic keratocyst (OKC) were included in the study. Slides were made from the archival blocks of each case and were stained immunohistochemically with calretinin. RESULTS: Correlation between calretinin staining and histopathological diagnosis was done, and it was found that all twenty cases of ameloblastoma showed positivity for calretinin, whereas 17 of twenty cases of unicystic ameloblastoma showed positivity for calretinin staining. All the cases of OKC and dentigerous cyst were negative for calretinin. CONCLUSION: Calretinin may serve as an important diagnostic adjunct in the differential diagnosis of ameloblastoma and cystic odontogenic lesions. Wolters Kluwer - Medknow 2020 2020-12-16 /pmc/articles/PMC8051659/ /pubmed/33897185 http://dx.doi.org/10.4103/njms.NJMS_71_19 Text en Copyright: © 2020 National Journal of Maxillofacial Surgery https://creativecommons.org/licenses/by-nc-sa/4.0/This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
spellingShingle Original Article
Varshney, Anchal
Aggarwal, Shivani
Gill, Sharanjeet Kaur
Aggarwal, Ashim
Jaiswal, Yashmi
Sharma, Jaishree
Comparison of calretinin expression in dentigerous cysts and ameloblastoma: An immunohistochemical study
title Comparison of calretinin expression in dentigerous cysts and ameloblastoma: An immunohistochemical study
title_full Comparison of calretinin expression in dentigerous cysts and ameloblastoma: An immunohistochemical study
title_fullStr Comparison of calretinin expression in dentigerous cysts and ameloblastoma: An immunohistochemical study
title_full_unstemmed Comparison of calretinin expression in dentigerous cysts and ameloblastoma: An immunohistochemical study
title_short Comparison of calretinin expression in dentigerous cysts and ameloblastoma: An immunohistochemical study
title_sort comparison of calretinin expression in dentigerous cysts and ameloblastoma: an immunohistochemical study
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8051659/
https://www.ncbi.nlm.nih.gov/pubmed/33897185
http://dx.doi.org/10.4103/njms.NJMS_71_19
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