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Trichomonas vaginalis infection impairs anion secretion in vaginal epithelium

Trichomonas vaginalis is a common protozoan parasite, which causes trichomoniasis associated with severe adverse reproductive outcomes. However, the underlying pathogenesis has not been fully understood. As the first line of defense against invading pathogens, the vaginal epithelial cells are highly...

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Autores principales: Xu, Jian-Bang, Lu, Shen-Jiao, Ke, Li-Jiao, Qiu, Zhuo-Er, Chen, Lei, Zhang, Hao-Li, Wang, Xi-Yuan, Wei, Xiao-Fan, He, Shuming, Zhu, Yun-Xin, Lun, Zhao-Rong, Zhou, Wen-Liang, Zhang, Yi-Lin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8051796/
https://www.ncbi.nlm.nih.gov/pubmed/33861752
http://dx.doi.org/10.1371/journal.pntd.0009319
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author Xu, Jian-Bang
Lu, Shen-Jiao
Ke, Li-Jiao
Qiu, Zhuo-Er
Chen, Lei
Zhang, Hao-Li
Wang, Xi-Yuan
Wei, Xiao-Fan
He, Shuming
Zhu, Yun-Xin
Lun, Zhao-Rong
Zhou, Wen-Liang
Zhang, Yi-Lin
author_facet Xu, Jian-Bang
Lu, Shen-Jiao
Ke, Li-Jiao
Qiu, Zhuo-Er
Chen, Lei
Zhang, Hao-Li
Wang, Xi-Yuan
Wei, Xiao-Fan
He, Shuming
Zhu, Yun-Xin
Lun, Zhao-Rong
Zhou, Wen-Liang
Zhang, Yi-Lin
author_sort Xu, Jian-Bang
collection PubMed
description Trichomonas vaginalis is a common protozoan parasite, which causes trichomoniasis associated with severe adverse reproductive outcomes. However, the underlying pathogenesis has not been fully understood. As the first line of defense against invading pathogens, the vaginal epithelial cells are highly responsive to environmental stimuli and contribute to the formation of the optimal luminal fluid microenvironment. The cystic fibrosis transmembrane conductance regulator (CFTR), an anion channel widely distributed at the apical membrane of epithelial cells, plays a crucial role in mediating the secretion of Cl(−) and HCO(3)(−). In this study, we investigated the effect of T. vaginalis on vaginal epithelial ion transport elicited by prostaglandin E(2) (PGE(2)), a major prostaglandin in the semen. Luminal administration of PGE(2) triggered a remarkable and sustained increase of short-circuit current (I(SC)) in rat vaginal epithelium, which was mainly due to Cl(−) and HCO(3)(−) secretion mediated by the cAMP-activated CFTR. However, T. vaginalis infection significantly abrogated the I(SC) response evoked by PGE(2), indicating impaired transepithelial anion transport via CFTR. Using a primary cell culture system of rat vaginal epithelium and a human vaginal epithelial cell line, we demonstrated that the expression of CFTR was significantly down-regulated after T. vaginalis infection. In addition, defective Cl(−) transport function of CFTR was observed in T. vaginalis-infected cells by measuring intracellular Cl(−) signals. Conclusively, T. vaginalis restrained exogenous PGE(2)-induced anion secretion through down-regulation of CFTR in vaginal epithelium. These results provide novel insights into the intervention of reproductive complications associated with T. vaginalis infection such as infertility and disequilibrium in vaginal fluid microenvironment.
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spelling pubmed-80517962021-04-28 Trichomonas vaginalis infection impairs anion secretion in vaginal epithelium Xu, Jian-Bang Lu, Shen-Jiao Ke, Li-Jiao Qiu, Zhuo-Er Chen, Lei Zhang, Hao-Li Wang, Xi-Yuan Wei, Xiao-Fan He, Shuming Zhu, Yun-Xin Lun, Zhao-Rong Zhou, Wen-Liang Zhang, Yi-Lin PLoS Negl Trop Dis Research Article Trichomonas vaginalis is a common protozoan parasite, which causes trichomoniasis associated with severe adverse reproductive outcomes. However, the underlying pathogenesis has not been fully understood. As the first line of defense against invading pathogens, the vaginal epithelial cells are highly responsive to environmental stimuli and contribute to the formation of the optimal luminal fluid microenvironment. The cystic fibrosis transmembrane conductance regulator (CFTR), an anion channel widely distributed at the apical membrane of epithelial cells, plays a crucial role in mediating the secretion of Cl(−) and HCO(3)(−). In this study, we investigated the effect of T. vaginalis on vaginal epithelial ion transport elicited by prostaglandin E(2) (PGE(2)), a major prostaglandin in the semen. Luminal administration of PGE(2) triggered a remarkable and sustained increase of short-circuit current (I(SC)) in rat vaginal epithelium, which was mainly due to Cl(−) and HCO(3)(−) secretion mediated by the cAMP-activated CFTR. However, T. vaginalis infection significantly abrogated the I(SC) response evoked by PGE(2), indicating impaired transepithelial anion transport via CFTR. Using a primary cell culture system of rat vaginal epithelium and a human vaginal epithelial cell line, we demonstrated that the expression of CFTR was significantly down-regulated after T. vaginalis infection. In addition, defective Cl(−) transport function of CFTR was observed in T. vaginalis-infected cells by measuring intracellular Cl(−) signals. Conclusively, T. vaginalis restrained exogenous PGE(2)-induced anion secretion through down-regulation of CFTR in vaginal epithelium. These results provide novel insights into the intervention of reproductive complications associated with T. vaginalis infection such as infertility and disequilibrium in vaginal fluid microenvironment. Public Library of Science 2021-04-16 /pmc/articles/PMC8051796/ /pubmed/33861752 http://dx.doi.org/10.1371/journal.pntd.0009319 Text en © 2021 Xu et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Xu, Jian-Bang
Lu, Shen-Jiao
Ke, Li-Jiao
Qiu, Zhuo-Er
Chen, Lei
Zhang, Hao-Li
Wang, Xi-Yuan
Wei, Xiao-Fan
He, Shuming
Zhu, Yun-Xin
Lun, Zhao-Rong
Zhou, Wen-Liang
Zhang, Yi-Lin
Trichomonas vaginalis infection impairs anion secretion in vaginal epithelium
title Trichomonas vaginalis infection impairs anion secretion in vaginal epithelium
title_full Trichomonas vaginalis infection impairs anion secretion in vaginal epithelium
title_fullStr Trichomonas vaginalis infection impairs anion secretion in vaginal epithelium
title_full_unstemmed Trichomonas vaginalis infection impairs anion secretion in vaginal epithelium
title_short Trichomonas vaginalis infection impairs anion secretion in vaginal epithelium
title_sort trichomonas vaginalis infection impairs anion secretion in vaginal epithelium
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8051796/
https://www.ncbi.nlm.nih.gov/pubmed/33861752
http://dx.doi.org/10.1371/journal.pntd.0009319
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