Cargando…
Field and classroom initiatives for portable sequence-based monitoring of dengue virus in Brazil
Brazil experienced a large dengue virus (DENV) epidemic in 2019, highlighting a continuous struggle with effective control and public health preparedness. Using Oxford Nanopore sequencing, we led field and classroom initiatives for the monitoring of DENV in Brazil, generating 227 novel genome sequen...
| Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group UK
2021
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8052316/ https://www.ncbi.nlm.nih.gov/pubmed/33863880 http://dx.doi.org/10.1038/s41467-021-22607-0 |
| _version_ | 1783679894480224256 |
|---|---|
| author | Adelino, Talita Émile Ribeiro Giovanetti, Marta Fonseca, Vagner Xavier, Joilson de Abreu, Álvaro Salgado do Nascimento, Valdinete Alves Demarchi, Luiz Henrique Ferraz Oliveira, Marluce Aparecida Assunção da Silva, Vinícius Lemes de Mello, Arabela Leal e. Silva Cunha, Gabriel Muricy Santos, Roselene Hans de Oliveira, Elaine Cristina Júnior, Jorge Antônio Chamon de Melo Iani, Felipe Campos de Filippis, Ana Maria Bispo de Abreu, André Luiz de Jesus, Ronaldo de Albuquerque, Carlos Frederico Campelo Rico, Jairo Mendez do Carmo Said, Rodrigo Fabiano Silva, Joscélio Aguiar de Moura, Noely Fabiana Oliveira Leite, Priscila Frutuoso, Lívia Carla Vinhal Haddad, Simone Kashima Martínez, Alexander Barreto, Fernanda Khouri Vazquez, Cynthia Carolina da Cunha, Rivaldo Venâncio Araújo, Emerson Luiz Lima de Oliveira Tosta, Stephane Fraga de Araújo Fabri, Allison Chalhoub, Flávia Löwen Levy da Silva Lemos, Poliana de Bruycker-Nogueira, Fernanda de Castro Lichs, Gislene Garcia Zardin, Marina Castilhos Souza Umaki Segovia, Fátima María Cardozo Gonçalves, Crhistinne Cavalheiro Maymone Grillo, Zoraida Del Carmen Fernandez Slavov, Svetoslav Nanev Pereira, Luiz Augusto Mendonça, Ana Flávia Pereira, Felicidade Mota de Magalhães, Jurandy Júnior Ferraz dos Santos Júnior, Agenor de Castro Moreira de Lima, Maricélia Maia Nogueira, Rita Maria Ribeiro Góes-Neto, Aristóteles de Carvalho Azevedo, Vasco Ariston Ramalho, Dario Brock Oliveira, Wanderson Kleber Macario, Eduardo Marques de Medeiros, Arnaldo Correia Pimentel, Victor Holmes, Edward C. de Oliveira, Tulio Lourenço, José Alcantara, Luiz Carlos Junior |
| author_facet | Adelino, Talita Émile Ribeiro Giovanetti, Marta Fonseca, Vagner Xavier, Joilson de Abreu, Álvaro Salgado do Nascimento, Valdinete Alves Demarchi, Luiz Henrique Ferraz Oliveira, Marluce Aparecida Assunção da Silva, Vinícius Lemes de Mello, Arabela Leal e. Silva Cunha, Gabriel Muricy Santos, Roselene Hans de Oliveira, Elaine Cristina Júnior, Jorge Antônio Chamon de Melo Iani, Felipe Campos de Filippis, Ana Maria Bispo de Abreu, André Luiz de Jesus, Ronaldo de Albuquerque, Carlos Frederico Campelo Rico, Jairo Mendez do Carmo Said, Rodrigo Fabiano Silva, Joscélio Aguiar de Moura, Noely Fabiana Oliveira Leite, Priscila Frutuoso, Lívia Carla Vinhal Haddad, Simone Kashima Martínez, Alexander Barreto, Fernanda Khouri Vazquez, Cynthia Carolina da Cunha, Rivaldo Venâncio Araújo, Emerson Luiz Lima de Oliveira Tosta, Stephane Fraga de Araújo Fabri, Allison Chalhoub, Flávia Löwen Levy da Silva Lemos, Poliana de Bruycker-Nogueira, Fernanda de Castro Lichs, Gislene Garcia Zardin, Marina Castilhos Souza Umaki Segovia, Fátima María Cardozo Gonçalves, Crhistinne Cavalheiro Maymone Grillo, Zoraida Del Carmen Fernandez Slavov, Svetoslav Nanev Pereira, Luiz Augusto Mendonça, Ana Flávia Pereira, Felicidade Mota de Magalhães, Jurandy Júnior Ferraz dos Santos Júnior, Agenor de Castro Moreira de Lima, Maricélia Maia Nogueira, Rita Maria Ribeiro Góes-Neto, Aristóteles de Carvalho Azevedo, Vasco Ariston Ramalho, Dario Brock Oliveira, Wanderson Kleber Macario, Eduardo Marques de Medeiros, Arnaldo Correia Pimentel, Victor Holmes, Edward C. de Oliveira, Tulio Lourenço, José Alcantara, Luiz Carlos Junior |
| author_sort | Adelino, Talita Émile Ribeiro |
| collection | PubMed |
| description | Brazil experienced a large dengue virus (DENV) epidemic in 2019, highlighting a continuous struggle with effective control and public health preparedness. Using Oxford Nanopore sequencing, we led field and classroom initiatives for the monitoring of DENV in Brazil, generating 227 novel genome sequences of DENV1-2 from 85 municipalities (2015–2019). This equated to an over 50% increase in the number of DENV genomes from Brazil available in public databases. Using both phylogenetic and epidemiological models we retrospectively reconstructed the recent transmission history of DENV1-2. Phylogenetic analysis revealed complex patterns of transmission, with both lineage co-circulation and replacement. We identified two lineages within the DENV2 BR-4 clade, for which we estimated the effective reproduction number and pattern of seasonality. Overall, the surveillance outputs and training initiative described here serve as a proof-of-concept for the utility of real-time portable sequencing for research and local capacity building in the genomic surveillance of emerging viruses. |
| format | Online Article Text |
| id | pubmed-8052316 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2021 |
| publisher | Nature Publishing Group UK |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-80523162021-04-30 Field and classroom initiatives for portable sequence-based monitoring of dengue virus in Brazil Adelino, Talita Émile Ribeiro Giovanetti, Marta Fonseca, Vagner Xavier, Joilson de Abreu, Álvaro Salgado do Nascimento, Valdinete Alves Demarchi, Luiz Henrique Ferraz Oliveira, Marluce Aparecida Assunção da Silva, Vinícius Lemes de Mello, Arabela Leal e. Silva Cunha, Gabriel Muricy Santos, Roselene Hans de Oliveira, Elaine Cristina Júnior, Jorge Antônio Chamon de Melo Iani, Felipe Campos de Filippis, Ana Maria Bispo de Abreu, André Luiz de Jesus, Ronaldo de Albuquerque, Carlos Frederico Campelo Rico, Jairo Mendez do Carmo Said, Rodrigo Fabiano Silva, Joscélio Aguiar de Moura, Noely Fabiana Oliveira Leite, Priscila Frutuoso, Lívia Carla Vinhal Haddad, Simone Kashima Martínez, Alexander Barreto, Fernanda Khouri Vazquez, Cynthia Carolina da Cunha, Rivaldo Venâncio Araújo, Emerson Luiz Lima de Oliveira Tosta, Stephane Fraga de Araújo Fabri, Allison Chalhoub, Flávia Löwen Levy da Silva Lemos, Poliana de Bruycker-Nogueira, Fernanda de Castro Lichs, Gislene Garcia Zardin, Marina Castilhos Souza Umaki Segovia, Fátima María Cardozo Gonçalves, Crhistinne Cavalheiro Maymone Grillo, Zoraida Del Carmen Fernandez Slavov, Svetoslav Nanev Pereira, Luiz Augusto Mendonça, Ana Flávia Pereira, Felicidade Mota de Magalhães, Jurandy Júnior Ferraz dos Santos Júnior, Agenor de Castro Moreira de Lima, Maricélia Maia Nogueira, Rita Maria Ribeiro Góes-Neto, Aristóteles de Carvalho Azevedo, Vasco Ariston Ramalho, Dario Brock Oliveira, Wanderson Kleber Macario, Eduardo Marques de Medeiros, Arnaldo Correia Pimentel, Victor Holmes, Edward C. de Oliveira, Tulio Lourenço, José Alcantara, Luiz Carlos Junior Nat Commun Article Brazil experienced a large dengue virus (DENV) epidemic in 2019, highlighting a continuous struggle with effective control and public health preparedness. Using Oxford Nanopore sequencing, we led field and classroom initiatives for the monitoring of DENV in Brazil, generating 227 novel genome sequences of DENV1-2 from 85 municipalities (2015–2019). This equated to an over 50% increase in the number of DENV genomes from Brazil available in public databases. Using both phylogenetic and epidemiological models we retrospectively reconstructed the recent transmission history of DENV1-2. Phylogenetic analysis revealed complex patterns of transmission, with both lineage co-circulation and replacement. We identified two lineages within the DENV2 BR-4 clade, for which we estimated the effective reproduction number and pattern of seasonality. Overall, the surveillance outputs and training initiative described here serve as a proof-of-concept for the utility of real-time portable sequencing for research and local capacity building in the genomic surveillance of emerging viruses. Nature Publishing Group UK 2021-04-16 /pmc/articles/PMC8052316/ /pubmed/33863880 http://dx.doi.org/10.1038/s41467-021-22607-0 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
| spellingShingle | Article Adelino, Talita Émile Ribeiro Giovanetti, Marta Fonseca, Vagner Xavier, Joilson de Abreu, Álvaro Salgado do Nascimento, Valdinete Alves Demarchi, Luiz Henrique Ferraz Oliveira, Marluce Aparecida Assunção da Silva, Vinícius Lemes de Mello, Arabela Leal e. Silva Cunha, Gabriel Muricy Santos, Roselene Hans de Oliveira, Elaine Cristina Júnior, Jorge Antônio Chamon de Melo Iani, Felipe Campos de Filippis, Ana Maria Bispo de Abreu, André Luiz de Jesus, Ronaldo de Albuquerque, Carlos Frederico Campelo Rico, Jairo Mendez do Carmo Said, Rodrigo Fabiano Silva, Joscélio Aguiar de Moura, Noely Fabiana Oliveira Leite, Priscila Frutuoso, Lívia Carla Vinhal Haddad, Simone Kashima Martínez, Alexander Barreto, Fernanda Khouri Vazquez, Cynthia Carolina da Cunha, Rivaldo Venâncio Araújo, Emerson Luiz Lima de Oliveira Tosta, Stephane Fraga de Araújo Fabri, Allison Chalhoub, Flávia Löwen Levy da Silva Lemos, Poliana de Bruycker-Nogueira, Fernanda de Castro Lichs, Gislene Garcia Zardin, Marina Castilhos Souza Umaki Segovia, Fátima María Cardozo Gonçalves, Crhistinne Cavalheiro Maymone Grillo, Zoraida Del Carmen Fernandez Slavov, Svetoslav Nanev Pereira, Luiz Augusto Mendonça, Ana Flávia Pereira, Felicidade Mota de Magalhães, Jurandy Júnior Ferraz dos Santos Júnior, Agenor de Castro Moreira de Lima, Maricélia Maia Nogueira, Rita Maria Ribeiro Góes-Neto, Aristóteles de Carvalho Azevedo, Vasco Ariston Ramalho, Dario Brock Oliveira, Wanderson Kleber Macario, Eduardo Marques de Medeiros, Arnaldo Correia Pimentel, Victor Holmes, Edward C. de Oliveira, Tulio Lourenço, José Alcantara, Luiz Carlos Junior Field and classroom initiatives for portable sequence-based monitoring of dengue virus in Brazil |
| title | Field and classroom initiatives for portable sequence-based monitoring of dengue virus in Brazil |
| title_full | Field and classroom initiatives for portable sequence-based monitoring of dengue virus in Brazil |
| title_fullStr | Field and classroom initiatives for portable sequence-based monitoring of dengue virus in Brazil |
| title_full_unstemmed | Field and classroom initiatives for portable sequence-based monitoring of dengue virus in Brazil |
| title_short | Field and classroom initiatives for portable sequence-based monitoring of dengue virus in Brazil |
| title_sort | field and classroom initiatives for portable sequence-based monitoring of dengue virus in brazil |
| topic | Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8052316/ https://www.ncbi.nlm.nih.gov/pubmed/33863880 http://dx.doi.org/10.1038/s41467-021-22607-0 |
| work_keys_str_mv | AT adelinotalitaemileribeiro fieldandclassroominitiativesforportablesequencebasedmonitoringofdenguevirusinbrazil AT giovanettimarta fieldandclassroominitiativesforportablesequencebasedmonitoringofdenguevirusinbrazil AT fonsecavagner fieldandclassroominitiativesforportablesequencebasedmonitoringofdenguevirusinbrazil AT xavierjoilson fieldandclassroominitiativesforportablesequencebasedmonitoringofdenguevirusinbrazil AT deabreualvarosalgado fieldandclassroominitiativesforportablesequencebasedmonitoringofdenguevirusinbrazil AT donascimentovaldinetealves fieldandclassroominitiativesforportablesequencebasedmonitoringofdenguevirusinbrazil AT demarchiluizhenriqueferraz fieldandclassroominitiativesforportablesequencebasedmonitoringofdenguevirusinbrazil AT oliveiramarluceaparecidaassuncao fieldandclassroominitiativesforportablesequencebasedmonitoringofdenguevirusinbrazil AT dasilvaviniciuslemes fieldandclassroominitiativesforportablesequencebasedmonitoringofdenguevirusinbrazil AT demelloarabelalealesilva fieldandclassroominitiativesforportablesequencebasedmonitoringofdenguevirusinbrazil AT cunhagabrielmuricy fieldandclassroominitiativesforportablesequencebasedmonitoringofdenguevirusinbrazil AT santosroselenehans fieldandclassroominitiativesforportablesequencebasedmonitoringofdenguevirusinbrazil AT deoliveiraelainecristina fieldandclassroominitiativesforportablesequencebasedmonitoringofdenguevirusinbrazil AT juniorjorgeantoniochamon fieldandclassroominitiativesforportablesequencebasedmonitoringofdenguevirusinbrazil AT demeloianifelipecampos fieldandclassroominitiativesforportablesequencebasedmonitoringofdenguevirusinbrazil AT defilippisanamariabispo fieldandclassroominitiativesforportablesequencebasedmonitoringofdenguevirusinbrazil AT deabreuandreluiz fieldandclassroominitiativesforportablesequencebasedmonitoringofdenguevirusinbrazil AT dejesusronaldo fieldandclassroominitiativesforportablesequencebasedmonitoringofdenguevirusinbrazil AT dealbuquerquecarlosfredericocampelo fieldandclassroominitiativesforportablesequencebasedmonitoringofdenguevirusinbrazil AT ricojairomendez fieldandclassroominitiativesforportablesequencebasedmonitoringofdenguevirusinbrazil AT docarmosaidrodrigofabiano fieldandclassroominitiativesforportablesequencebasedmonitoringofdenguevirusinbrazil AT silvajoscelioaguiar fieldandclassroominitiativesforportablesequencebasedmonitoringofdenguevirusinbrazil AT demouranoelyfabianaoliveira fieldandclassroominitiativesforportablesequencebasedmonitoringofdenguevirusinbrazil AT leitepriscila fieldandclassroominitiativesforportablesequencebasedmonitoringofdenguevirusinbrazil AT frutuosoliviacarlavinhal fieldandclassroominitiativesforportablesequencebasedmonitoringofdenguevirusinbrazil AT haddadsimonekashima fieldandclassroominitiativesforportablesequencebasedmonitoringofdenguevirusinbrazil AT martinezalexander fieldandclassroominitiativesforportablesequencebasedmonitoringofdenguevirusinbrazil AT barretofernandakhouri fieldandclassroominitiativesforportablesequencebasedmonitoringofdenguevirusinbrazil AT vazquezcynthiacarolina fieldandclassroominitiativesforportablesequencebasedmonitoringofdenguevirusinbrazil AT dacunharivaldovenancio fieldandclassroominitiativesforportablesequencebasedmonitoringofdenguevirusinbrazil AT araujoemersonluizlima fieldandclassroominitiativesforportablesequencebasedmonitoringofdenguevirusinbrazil AT deoliveiratostastephanefraga fieldandclassroominitiativesforportablesequencebasedmonitoringofdenguevirusinbrazil AT dearaujofabriallison fieldandclassroominitiativesforportablesequencebasedmonitoringofdenguevirusinbrazil AT chalhoubflavialowenlevy fieldandclassroominitiativesforportablesequencebasedmonitoringofdenguevirusinbrazil AT dasilvalemospoliana fieldandclassroominitiativesforportablesequencebasedmonitoringofdenguevirusinbrazil AT debruyckernogueirafernanda fieldandclassroominitiativesforportablesequencebasedmonitoringofdenguevirusinbrazil AT decastrolichsgislenegarcia fieldandclassroominitiativesforportablesequencebasedmonitoringofdenguevirusinbrazil AT zardinmarinacastilhossouzaumaki fieldandclassroominitiativesforportablesequencebasedmonitoringofdenguevirusinbrazil AT segoviafatimamariacardozo fieldandclassroominitiativesforportablesequencebasedmonitoringofdenguevirusinbrazil AT goncalvescrhistinnecavalheiromaymone fieldandclassroominitiativesforportablesequencebasedmonitoringofdenguevirusinbrazil AT grillozoraidadelcarmenfernandez fieldandclassroominitiativesforportablesequencebasedmonitoringofdenguevirusinbrazil AT slavovsvetoslavnanev fieldandclassroominitiativesforportablesequencebasedmonitoringofdenguevirusinbrazil AT pereiraluizaugusto fieldandclassroominitiativesforportablesequencebasedmonitoringofdenguevirusinbrazil AT mendoncaanaflavia fieldandclassroominitiativesforportablesequencebasedmonitoringofdenguevirusinbrazil AT pereirafelicidademota fieldandclassroominitiativesforportablesequencebasedmonitoringofdenguevirusinbrazil AT demagalhaesjurandyjuniorferraz fieldandclassroominitiativesforportablesequencebasedmonitoringofdenguevirusinbrazil AT dossantosjunioragenordecastromoreira fieldandclassroominitiativesforportablesequencebasedmonitoringofdenguevirusinbrazil AT delimamariceliamaia fieldandclassroominitiativesforportablesequencebasedmonitoringofdenguevirusinbrazil AT nogueiraritamariaribeiro fieldandclassroominitiativesforportablesequencebasedmonitoringofdenguevirusinbrazil AT goesnetoaristoteles fieldandclassroominitiativesforportablesequencebasedmonitoringofdenguevirusinbrazil AT decarvalhoazevedovascoariston fieldandclassroominitiativesforportablesequencebasedmonitoringofdenguevirusinbrazil AT ramalhodariobrock fieldandclassroominitiativesforportablesequencebasedmonitoringofdenguevirusinbrazil AT oliveirawandersonkleber fieldandclassroominitiativesforportablesequencebasedmonitoringofdenguevirusinbrazil AT macarioeduardomarques fieldandclassroominitiativesforportablesequencebasedmonitoringofdenguevirusinbrazil AT demedeirosarnaldocorreia fieldandclassroominitiativesforportablesequencebasedmonitoringofdenguevirusinbrazil AT pimentelvictor fieldandclassroominitiativesforportablesequencebasedmonitoringofdenguevirusinbrazil AT fieldandclassroominitiativesforportablesequencebasedmonitoringofdenguevirusinbrazil AT holmesedwardc fieldandclassroominitiativesforportablesequencebasedmonitoringofdenguevirusinbrazil AT deoliveiratulio fieldandclassroominitiativesforportablesequencebasedmonitoringofdenguevirusinbrazil AT lourencojose fieldandclassroominitiativesforportablesequencebasedmonitoringofdenguevirusinbrazil AT alcantaraluizcarlosjunior fieldandclassroominitiativesforportablesequencebasedmonitoringofdenguevirusinbrazil |